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AB197793

Anti-NAT8 antibody

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(1 Publication)

Rabbit Polyclonal NAT8 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAT8 aa 50 to C-terminus.

View Alternative Names

CML1, GLA, TSC501, NAT8, N-acetyltransferase 8, Acetyltransferase 2, Camello-like protein 1, Cysteinyl-conjugate N-acetyltransferase, ATase2, CCNAT

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT8 antibody (AB197793)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT8 antibody (AB197793)

Immunohistochemical analysis of paraffin-embedded Human cervical tissue labeling NAT8 using ab197793 at a 1/50 dilution.

Western blot - Anti-NAT8 antibody (AB197793)
  • WB

Supplier Data

Western blot - Anti-NAT8 antibody (AB197793)

All lanes:

Western blot - Anti-NAT8 antibody (ab197793) at 1/238 dilution

Lane 1:

mouse kidney tissue lysate at 40 µg

Lane 2:

mouse liver tissue lysate at 40 µg

Predicted band size: 26 kDa

false

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NAT8 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UHE5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

{NAT8} refers to N-acetyltransferase 8 also known as N-acetyltransferase type I. This enzyme's mass is approximately 29 kDa. {NAT8} is expressed mainly in the liver and kidneys. It belongs to a family of N-acetyltransferases catalyzing the transfer of the acetyl group from acetyl-CoA to various substrates. This activity helps in xenobiotic metabolism enhancing water solubility for excretion.
Biological function summary

{NAT8} participates in the detoxification process by transferring an acetyl group to endogenous amines and drugs. Acetylation by {NAT8} facilitates these compounds' excretion serving a protective role against potentially hazardous substances. {NAT8} does not generally form part of a larger protein complex but its enzymatic function is reliant upon the presence of acetyl-CoA as a cofactor.

Pathways

The acetylation function of {NAT8} integrates into metabolic and detoxification pathways. It plays a role in the biotransformation pathway operating with enzymes like CYP450 that handle phase I and phase II metabolism of various xenobiotics. In this capacity {NAT8} works alongside other acetyltransferases contributing to the comprehensive process of making drugs and toxins more hydrophilic.

Researchers have identified a connection between {NAT8} and conditions affecting the kidney such as renal failure. Aberrant expression or activity of {NAT8} may correlate with disease processes influencing drug metabolism. Notably polymorphisms in {NAT8} could affect interaction with other proteins involved in kidney function such as nephrin potentially impacting disease progression and treatment outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids (PubMed : 20392701). This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1 (PubMed : 24556617). May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (PubMed : 19011241).
See full target information NAT8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39255019

2024

Glial ferritin maintains neural stem cells via transporting iron required for self-renewal in .

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Ma,Wenshu Wang,Xiaojing Yang,Menglong Rui,Su Wang
View all publications
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