Rabbit Recombinant Monoclonal proANP antibody. Carrier free. Suitable for mIHC, IP, WB, IHC-Fr, IHC-P and reacts with Human, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44919.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873)")
![Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunohistochemistry-frozen-sections-img123299.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873)")
![Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunoprecipitation-img61371.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118490.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99276.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (AB225873)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Tested |
| Rat | Expected | Tested | Expected | Tested | Expected |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Atrial natriuretic peptide. Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism (PubMed:15741263, PubMed:16875975, PubMed:18835931, PubMed:21672517, PubMed:22307324, PubMed:2532366, PubMed:2825692, PubMed:7595132, PubMed:7720651, PubMed:8087923, PubMed:8653797). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses (PubMed:1660465, PubMed:1672777, PubMed:21098034, PubMed:2162527, PubMed:22307324, PubMed:25401746, PubMed:2825692, PubMed:7720651, PubMed:8384600, PubMed:9893117). Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance (PubMed:2532366, PubMed:2825692, PubMed:7595132, PubMed:7720651, PubMed:8087923, PubMed:8653797). Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts (PubMed:16875975). Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension (By similarity). In adipose tissue, acts in various cGMP- and PKG-dependent pathways to regulate lipid metabolism and energy homeostasis (PubMed:15741263, PubMed:18835931, PubMed:21672517, PubMed:22307324). This includes up-regulating lipid metabolism and mitochondrial oxygen utilization by activating the AMP-activated protein kinase (AMPK), and increasing energy expenditure by acting via MAPK11 to promote the UCP1-dependent thermogenesis of brown adipose tissue (PubMed:15741263, PubMed:18835931, PubMed:21672517, PubMed:22307324). Binds the clearance receptor NPR3 which removes the hormone from circulation (PubMed:1672777). Long-acting natriuretic peptide. May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, vasodilation, and inhibiting aldosterone synthesis (PubMed:2532366, PubMed:2825692, PubMed:7595132, PubMed:7955907, PubMed:8087923, PubMed:8653797). In vitro, promotes the production of cGMP and induces vasodilation (PubMed:2825692). May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase (PubMed:7720651). However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting; according to a report, in vivo it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis (By similarity). Appears to bind to specific receptors that are distinct from the receptors bound by atrial natriuretic peptide and vessel dilator (PubMed:2162527, PubMed:2825692). Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption (PubMed:11145122). Vessel dilator. May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, and vasodilation (PubMed:2532366, PubMed:7595132, PubMed:7955907, PubMed:8087923, PubMed:8653797). In vitro, promotes the production of cGMP and induces vasodilation (PubMed:2825692). May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase (PubMed:7595132, PubMed:7720651). However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting; according to a report it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis (PubMed:7831500). Appears to bind to specific receptors that are distinct from the receptors bound by the atrial natriuretic and long-acting natriuretic peptides (PubMed:2162527, PubMed:2825692). Possibly functions in protein excretion in urine by maintaining the integrity of the proximal tubules and enhancing protein excretion by decreasing proximal tubular protein reabsorption (PubMed:11145122). Kaliuretic peptide. May have a role in cardio-renal homeostasis through regulation of diuresis and inhibiting aldosterone synthesis (PubMed:2825692, PubMed:7595132, PubMed:8087923). In vitro, promotes the production of cGMP and induces vasodilation (PubMed:2825692). May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase (PubMed:7595132, PubMed:7720651). May have a role in potassium excretion but not sodium excretion (natriuresis) (PubMed:8087923). Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption (PubMed:11145122). Urodilatin. Hormone produced in the kidneys that appears to be important for maintaining cardio-renal homeostasis (PubMed:8351194, PubMed:8779891, PubMed:8853410). Mediates vasodilation, natriuresis and diuresis primarily in the renal system, in order to maintain the extracellular fluid volume and control the fluid-electrolyte balance (PubMed:2528951, PubMed:8351194, PubMed:8779891, PubMed:8853410). Specifically binds and stimulates cGMP production by renal transmembrane receptors, likely NPR1 (PubMed:8384600, PubMed:9893117). Urodilatin not ANP, may be the natriuretic peptide responsible for the regulation of sodium and water homeostasis in the kidney (PubMed:8384600, PubMed:8779891). Auriculin-D. May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips. Auriculin-B. May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips. Auriculin-A. May have a role in cardio-renal homeostasis through regulation of regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, vasodilates intestinal smooth muscle but not smooth muscle strips. Atriopeptin-2. May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal and vascular smooth muscle strips. Atriopeptin-1. May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal smooth muscle but not vascular smooth muscle strips.
Alternative names
ANP, PND, NPPA, Natriuretic peptides A, Atrial natriuretic factor prohormone, Atrial natriuretic peptide prohormone, Atriopeptigen, Cardiodilatin, preproCDD-ANF, proANF, preproANP, proANP, CDD
Recommended products
Rabbit Recombinant Monoclonal proANP antibody. Carrier free. Suitable for mIHC, IP, WB, IHC-Fr, IHC-P and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20247
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is not suitable to detect ANP in H9c2 cell line.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab225873 is the carrier-free version of Anti-Natriuretic peptides A antibody [EPR20247] ab209232.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Natriuretic Peptides A also known as ANP ANF or NPPA are important cardiac hormones made by atrial myocytes in the heart. This peptide with a mass of around 28 amino acids plays an important role mechanically in modulating blood pressure and fluid balance. It achieves this by binding to receptors that activate cyclic GMP (cGMP) production leading to vasodilation. ANP expression is mainly found in cardiac muscle cells but also detected in other tissues under certain conditions.
- Biological function summary
These peptides contribute to renal sodium excretion commonly known as natriuresis and also promote diuresis which increases urine volume. ANP functions as a singular hormone without forming part of a complex. Beyond its role in cardiovascular and renal systems ANP influences lipid metabolism and adipocyte functions which may have implications for metabolic health.
- Pathways
ANP is engaged in the natriuretic peptide signaling pathway which is important for cardiovascular homeostasis. Within this pathway related proteins include B-type Natriuretic Peptide (BNP) and C-type Natriuretic Peptide (CNP) all of which share similar receptor interactions through cGMP. Additionally ANP impacts the renin-angiotensin system (RAS) a significant hormonal system regulating blood pressure and fluid balance by counteracting the actions of angiotensin II.
- Associated diseases and disorders
ANP is notably associated with heart failure where its levels can indicate disease severity and prognosis. Elevated ANP levels have also been linked to hypertension contributing to diagnostic evaluations alongside other biomarkers like BNP. Both heart failure and hypertension involve complex interactions between ANP and proteins such as angiotensin II within their pathophysiological frameworks.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44919.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Natriuretic peptides A with Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic and perinuclear staining on human heart (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Natriuretic peptides A antibody [EPR20247] ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunohistochemistry-frozen-sections-img123299.jpg "Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on the auricula of rat heart (PMID: 2942710, PMID: 1824903).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Natriuretic peptides A antibody [EPR20247] ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunoprecipitation-img61371.jpg "Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Natriuretic peptides A was immunoprecipitated from 0.35 mg of rat heart lysate with Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Rat heart lysate 10 μg (Input).
Lane 2: Anti-Natriuretic peptides A antibody [EPR20247] ab209232 IP in rat heart lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Natriuretic peptides A antibody [EPR20247] ab209232 in rat heart lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Natriuretic peptides A antibody [EPR20247] ab209232).
All lanes: Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] (Anti-Natriuretic peptides A antibody [EPR20247] ab209232)
Predicted band size: 16 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118490.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Immunohistochemical analysis of paraffin-embedded rat auricle tissue labeling Natriuretic peptides A with Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic and perinuclear staining on rat auricle (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Natriuretic peptides A antibody [EPR20247] ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99276.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Immunohistochemical analysis of paraffin-embedded rat ventricle tissue labeling Natriuretic peptides A with Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Weak staining on rat ventricle (PMID: 2942710, PMID: 1824903).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Natriuretic peptides A antibody [EPR20247] ab209232).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--western-blot-img431750.jpg "Western blot - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Western blot - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
This data was developed using Anti-Natriuretic peptides A antibody [EPR20247] ab209232, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 GAPDH is used a loading control at 1/100000 dilution.
Although some references report that ANP is expressed in H9c2 (2-1) cell line (PMID: 31714667, PMID: 30483785), Anti-Natriuretic peptides A antibody [EPR20247] ab209232 is not suitable to detect ANP in this cell line.
All lanes: Western blot - Anti-Natriuretic peptides A antibody [EPR20247] (Anti-Natriuretic peptides A antibody [EPR20247] ab209232) at 1/10000 dilution
Lane 1: Untreated H9c2 (2-1) (rat cardiac myoblast cell) whole cell lysate at 20 µg
Lane 2: H9c2 (2-1) (rat cardiac myoblast cell) treated with LPS (2ug/ml, 16h) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Exposure time: 180s
![Multiplex immunohistochemistry - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--multiplex-immunohistochemistry-img420906.jpg "Multiplex immunohistochemistry - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
Multiplex immunohistochemistry - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)
Fluorescence multiplex immunohistochemical analysis of the human cardiac muscle (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-delta Sarcoglycan (Anti-delta Sarcoglycan antibody [EPR8706] ab137101, red; Opal™690), anti-Titin (Anti-Titin antibody [EPR26167-75] ab307446, green; Opal™520) and anti-Natriuretic peptides A (Anti-Natriuretic peptides A antibody [EPR20247] ab209232, magenta; Opal™570) on human cardiac muscle. Panel B: anti-Titin displayed nucleus and cytoplasm expression. Panel C: anti-Natriuretic peptides A displayed granular cytoplasmic expression. Panel D: anti-delta Sarcoglycan displayed membrane expression. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of Anti-delta Sarcoglycan antibody [EPR8706] ab137101 at 1/1000 (1.043 μg/ml) dilution, Anti-Titin antibody [EPR26167-75] ab307446 at 1/500 (0.95 μg/ml) dilution, and Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/3000 (0.241 μg/ml) dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Natriuretic peptides A antibody [EPR20247] ab209232).
![Western blot - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--western-blot-img431750.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
![Multiplex immunohistochemistry - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873), expandable thumbnail](https://content.abcam.com/products/images/natriuretic-peptides-a-antibody-epr20247-bsa-and-azide-free-ab225873--multiplex-immunohistochemistry-img420906.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)")
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