AB36392

Anti-Natural Killer Cell Activation Structures antibody [ANK61]

Name: Anti-Natural Killer Cell Activation Structures antibody [ANK61] (ab36392) | Abcam
Brand: Abcam Limited
SKU: AB36392

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(12 Publications)

Mouse Monoclonal Natural Killer Cell Activation Structures antibody. Suitable for IP, Flow Cyt, IHC-P, IHC-Fr and reacts with Rat samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing Natural Killer Cell Activation Structures protein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ANK61

Isotype

IgG1

Carrier free

Reacts with

Rat

Applications

Flow Cyt, IHC-P, IP, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antigen recognized by ab36392 is highly expressed on freshly isolated and cultured NK cells. The antibody also binds to rat alpha beta TCR T cells and at a low level to B cells.

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Reactivity data

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Product details

Triggering of the antigen by ab36392 antibody activates the lytic machinery of rat NK cells, but not of T cells.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Natural Killer (NK) Cell Activation Structures are essential components responsible for triggering the killing activity of NK cells. These structures are primarily located on the surface of NK cells where they interact with ligands on target cells. The mass of these activation structures can vary as they often comprise clusters of proteins such as CD16 also known as Fc receptor IIIA and their associated molecules. CD16 has an estimated molecular weight of around 50 kDa. NK cell activation structures are expressed across various tissues where immune surveillance is necessary.

Biological function summary

These structures serve to initiate the cytotoxic response of NK cells by recognizing stressed cells and those lacking "self" markers. When NK cell activation structures engage target cells they form part of a complex that includes proteins such as NKG2D and NKp46. These complexes recognize specific ligands on potential target cells facilitating the release of cytotoxic granules and cytokines. This biological function ensures that NK cells can target and eliminate infected or cancerous cells while sparing normal cells.

Pathways

NK cell activation structures play an important role in the immune response pathways specifically the natural cytotoxicity pathway. They interact with signaling molecules like DAP10 and DAP12 which transmit activation signals to the interior of the NK cells. This signal transduction enhances the ability of NK cells to respond to infections and cancerous changes. Another pathway is the Fc receptor pathway in which CD16 is associated with the activation of NK cells through antibody-dependent cellular cytotoxicity (ADCC).

Activating structures of NK cells have connections to conditions such as cancer and autoimmune diseases. In cancer defective NK cell activation can lead to insufficient immune surveillance allowing tumor cells to proliferate. Malfunctions in these structures potentially linked with proteins like MHC class I chain-related proteins can also result in an autoimmune response where NK cells mistakenly target healthy tissue. Understanding and targeting these structures might lead to improved therapeutic strategies for modulating immune responses in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Natural Killer Cell Activation Structures

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

American journal of reproductive immunology (New York, N.Y. : 1989) 92:e13895 PubMed39001587

2024

IL-33 Signaling Inhibition Leads to a Preeclampsia-Like Phenotype in Pregnant Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Corbin A Shields,Deanna Thompson,Jie McKay,Rachel Wilson,Marcus K Robbins,Hannah Glenn,Molly Fontenot,Jan M Williams,Denise C Cornelius

American journal of physiology. Heart and circulatory physiology 326:H1006-H1016 PubMed38363211

2024

IL-33 supplementation improves uterine artery resistance and maternal hypertension in response to placental ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Corbin Shields,Geilda Tardo,Greg Peacock,Emily Hester,Marissa Anderson,Jan M Williams,Denise C Cornelius

International journal of molecular sciences 25: PubMed38255935

2024

Inhibition of Caspase 1 Reduces Blood Pressure, Cytotoxic NK Cells, and Inflammatory T-Helper 17 Cells in Placental Ischemic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Corbin A Shields,Geilda A Tardo,Xi Wang,Gregory Peacock,Marcus Robbins,Hannah Glenn,Rachel Wilson,Jan M Williams,Denise C Cornelius

American journal of physiology. Regulatory, integrative and comparative physiology 324:R556-R567 PubMed36847598

2023

NLRP3 inhibition improves maternal hypertension, inflammation, and vascular dysfunction in response to placental ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Olivia K Travis,Corbin A Shields,G Ann Tardo,Chelsea Giachelli,Christopher W Nutter,Hannah L Glenn,Olive G Cooper,Tatiana Davis,Rashauna Thomas,Jan M Williams,Denise C Cornelius

American journal of physiology. Regulatory, integr : PubMed33533305

2021

Progesterone induced blocking factor improves blood pressure, inflammation and pup weight in response to reduced uterine perfusion pressure (RUPP).

Applications

Unspecified application

Species

Unspecified reactive species

Jesse N Cottrell,Alexis Witcher,Kyleigh M Comley,Mark W Cunningham,Tarek Ibrahim,Denise C Cornelius,Babbette D LaMarca,Lorena M Amaral

American journal of reproductive immunology (New York, N.Y. : 1989) 85:e13386 PubMed33315281

2020

Adoptive transfer of placental ischemia-stimulated natural killer cells causes a preeclampsia-like phenotype in pregnant rats.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia K Travis,Cedar Baik,Geilda A Tardo,Lorena Amaral,Carmilya Jackson,Mallory Greer,Chelsea Giachelli,Tarek Ibrahim,Owen T Herrock,Jan M Williams,Denise C Cornelius

American journal of physiology. Regulatory, integr 318:R1036-R1046 PubMed32320265

2020

Interleukin-17 signaling mediates cytolytic natural killer cell activation in response to placental ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia K Travis,Dakota White,Cedar Baik,Chelsea Giachelli,Willie Thompson,Cassandra Stubbs,Mallory Greer,James P Lemon,Jan Michael Williams,Denise C Cornelius

Physiological reports 7:e14038 PubMed30963715

2019

Chronic infusion of interleukin-17 promotes hypertension, activation of cytolytic natural killer cells, and vascular dysfunction in pregnant rats.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia K Travis,Dakota White,W Austin Pierce,Ying Ge,Cassandra Y Stubbs,Frank T Spradley,Jan M Williams,Denise C Cornelius

American journal of physiology. Regulatory, integrative and comparative physiology 316:R165-R171 PubMed30624978

2019

Interleukin-4 supplementation improves the pathophysiology of hypertension in response to placental ischemia in RUPP rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse N Cottrell,Lorena M Amaral,Ashlyn Harmon,Denise C Cornelius,Mark W Cunningham,Venkata Ramana Vaka,Tarek Ibrahim,Florian Herse,Gerd Wallukat,Ralf Dechend,Babbette LaMarca

American journal of physiology. Regulatory, integr 315:R336-R343 PubMed29718698

2018

Placental ischemia-stimulated T-helper 17 cells induce preeclampsia-associated cytolytic natural killer cells during pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Corbin A Shields,Maggie McCalmon,Tarek Ibrahim,Dakota L White,Jan M Williams,Babbette LaMarca,Denise C Cornelius

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Natural Killer Cell Activation Structures antibody [ANK61]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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Product promise