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Rabbit Monoclonal SCN5A antibody. Carrier free. Suitable for mIHC, WB, IHC-P and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049), expandable thumbnail
  • Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCWBIHC-PIPICC/IFFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Pore-forming subunit of Nav1.5, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed:11834499, PubMed:23420830). Nav1.5 is the predominant sodium channel expressed in myocardial cells and it is responsible for the initial upstroke of the action potential in cardiac myocytes, thereby initiating the heartbeat (PubMed:11834499, PubMed:23420830, PubMed:29306897). Required for normal electrical conduction including formation of the infranodal ventricular conduction system and normal action potential configuration, as a result of its interaction with XIRP2 (PubMed:29306897).

Alternative names

Sodium channel protein type 5 subunit alpha, Sodium channel protein cardiac muscle subunit alpha, Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, mH1, Scn5a

Recommended products

Rabbit Monoclonal SCN5A antibody. Carrier free. Suitable for mIHC, WB, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25136-48
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nav1.5 also known as SCN5A is a protein that functions mechanically as a voltage-gated sodium channel. This channel facilitates the rapid influx of sodium ions across the cell membrane leading to the depolarization necessary for action potential initiation and propagation in cardiac cells. Nav1.5 weighs approximately 220-260 kDa and is primarily expressed in cardiac myocytes where it plays a central role in maintaining cardiac excitability and conduction.

Biological function summary

The sodium channel Nav1.5 is integral to the electrical signaling within the heart. It functions within the cardiac cell membrane and forms part of the larger sodium channel complex. Nav1.5 maintains ionic balance and supports normal cardiac rhythm making it essential for the contraction of the heart. Its expression and function can be influenced by interacting proteins and intracellular signaling molecules.

Pathways

Nav1.5 is an important component in the cardiac action potential generation and conduction pathways. It directly influences the cardiac conduction system and is part of the pathway that encompasses the sodium-potassium pump and other ion channels like Nav1.1 and Nav1.8. Through these pathways Nav1.5 regulates cardiac rhythm and myocardial contractility ensuring efficient heart function.

Associated diseases and disorders

Nav1.5 mutations have significant associations with arrhythmias and cardiac conduction issues such as long QT syndrome and Brugada syndrome. These cardiac conditions are linked to disrupted sodium channel function caused by mutations in the SCN5A gene. Nav1.1 and Nav1.2 other family members of the sodium channel can sometimes show altered expression in these disorders reflecting the interconnected nature of this protein family in cardiac health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049), expandable thumbnail

    Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as a blocking and diluting buffer and concentration.

    Negative control: liver, spleen(PMID: 1309946)

    Samples are non-boiled as boiling may cause protein aggregates.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] (Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 226 kDa

    Observed band size: 250 kDa

    Exposure time: 3min

  • Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049), expandable thumbnail

    Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as a blocking and diluting buffer and concentration.

    Negative control: liver (PMID: 1309946).

    Samples are non-boiled as boiling may cause protein aggregates.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] (Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048) at 1/1000 dilution

    Lane 1: Rat heart tissue lysate at 20 µg

    Lane 2: Rat liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 226 kDa

    Observed band size: 250 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling Nav1.5/SCN5A with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 at 1/100 (4.98 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cardiac muscle (PMID: 23542581) (PMID 18178574). The section was incubated with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling Nav1.5/SCN5A with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 at 1/100 (4.98 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on mouse cardiac muscle (PMID: 23542581) (PMID 18178574). The section was incubated with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling Nav1.5/SCN5A with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 at 1/100 (4.98 μ/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control: no staining on rat liver (PMID: 22331407). The section was incubated with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling Nav1.5/SCN5A with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 at 1/100 (4.98 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on mouse spleen (PMID:1309946). The section was incubated with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 the same antibody clone in a different buffer formulation.

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cardiac muscle tissue staining Nav1.5 with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 at a 1/1000 (5.12 µg/ml), dilution A2BP1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

    Panel A: merged staining of anti-Nav1.5 (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse cardiac muscle.
    Panel B: anti-Nav1.5 staining cytoplasm in mouse cardiac muscle.
    Panel C: anti-A2BP1 staining nucleus in mouse cardiac muscle.
    Panel D: anti-Dystrophin staining membrane in mouse cardiac muscle.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (ab300049)

    This data was developed using Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 the same antibody clone in a different buffer formulation.

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining Nav1.5 with Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048 at a 1/1000 (5.12 µg/ml) dilution, A2BP1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

    Panel A: merged staining of anti-Nav1.5 (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
    Panel B: anti-Nav1.5 staining cytoplasm in mouse skeletal muscle.
    Panel C: anti-A2BP1 staining nucleus in mouse skeletal muscle.
    Panel D: anti-Dystrophin staining membrane in mouse skeletal muscle.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-Nav1.5/SCN5A antibody [EPR25136-48] ab300048, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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