Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049)

This data was developed using ab300048 the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining Nav1.5 with ab300048 at a 1/1000 (5.12 µg/ml) dilution, A2BP1 with ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

Panel A : merged staining of anti-Nav1.5 (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B : anti-Nav1.5 staining cytoplasm in mouse skeletal muscle.
Panel C : anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D : anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab300048, ab254413 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049)

This data was developed using ab300048, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling Nav1.5/SCN5A with ab300048 at 1/100 (4.98 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cardiac muscle (PMID : 23542581) (PMID 18178574). The section was incubated with ab300048 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049)

This data was developed using ab300048 the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cardiac muscle tissue staining Nav1.5 with ab300048 at a 1/1000 (5.12 µg/ml), dilution A2BP1 with ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

Panel A : merged staining of anti-Nav1.5 (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse cardiac muscle.
Panel B : anti-Nav1.5 staining cytoplasm in mouse cardiac muscle.
Panel C : anti-A2BP1 staining nucleus in mouse cardiac muscle.
Panel D : anti-Dystrophin staining membrane in mouse cardiac muscle.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab300048, ab254413 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049)

This data was developed using ab300048, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling Nav1.5/SCN5A with ab300048 at 1/100 (4.98 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control : no staining on mouse spleen (PMID : 1309946). The section was incubated with ab300048 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049)

This data was developed using ab300048, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling Nav1.5/SCN5A with ab300048 at 1/100 (4.98 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on mouse cardiac muscle (PMID : 23542581) (PMID 18178574). The section was incubated with ab300048 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049)

This data was developed using ab300048, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling Nav1.5/SCN5A with ab300048 at 1/100 (4.98 μ/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control : no staining on rat liver (PMID : 22331407). The section was incubated with ab300048 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049)

This data was developed using ab300048, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as a blocking and diluting buffer and concentration.

Negative control : liver, spleen(PMID : 1309946)

Samples are non-boiled as boiling may cause protein aggregates.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] (<a href='/en-us/products/primary-antibodies/nav15-scn5a-antibody-epr25136-48-ab300048'>ab300048</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 226 kDa

Observed band size: 250 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] - BSA and Azide free (AB300049)

This data was developed using ab300048, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as a blocking and diluting buffer and concentration.

Negative control : liver (PMID : 1309946).

Samples are non-boiled as boiling may cause protein aggregates.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-Nav1.5/SCN5A antibody [EPR25136-48] (<a href='/en-us/products/primary-antibodies/nav15-scn5a-antibody-epr25136-48-ab300048'>ab300048</a>) at 1/1000 dilution

Lane 1:

Rat heart tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 226 kDa

Observed band size: 250 kDa

false

Exposure time: 3min