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AB303658

Anti-Nav1.5/SCN5A antibody [N397/19] - BSA and Azide free

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Mouse Recombinant Monoclonal SCN5A antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

Sodium channel protein type 5 subunit alpha, Sodium channel protein cardiac muscle subunit alpha, Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, hH1, SCN5A

1 Images
Western blot - Anti-Nav1.5/SCN5A antibody [N397/19] - BSA and Azide free (AB303658)
  • WB

Supplier Data

Western blot - Anti-Nav1.5/SCN5A antibody [N397/19] - BSA and Azide free (AB303658)

This data was developed using ab303657, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen (PMID : 1309946). Samples are non-boiled as boiling may cause protein aggregates. This blot was developed using a higher sensitivity ECL substrate. Exposure time : 169 minutes

All lanes:

Western blot - Anti-Nav1.5/SCN5A antibody [N397/19] (<a href='/en-us/products/primary-antibodies/nav15-scn5a-antibody-n397-19-ab303657'>ab303657</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 40 µg

Lane 2:

Mouse spleen tissue lysate at 40 µg

Lane 3:

Rat heart tissue lysate at 40 µg

Lane 4:

Rat spleen tissue lysate at 40 µg

Secondary

All lanes:

Western blot at 1/5000 dilution

Observed band size: 260 kDa

true

Exposure time: 169min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N397/19

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab303658 is a carrier free version of ab303657.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nav1.5 also known as SCN5A is a protein that functions mechanically as a voltage-gated sodium channel. This channel facilitates the rapid influx of sodium ions across the cell membrane leading to the depolarization necessary for action potential initiation and propagation in cardiac cells. Nav1.5 weighs approximately 220-260 kDa and is primarily expressed in cardiac myocytes where it plays a central role in maintaining cardiac excitability and conduction.
Biological function summary

The sodium channel Nav1.5 is integral to the electrical signaling within the heart. It functions within the cardiac cell membrane and forms part of the larger sodium channel complex. Nav1.5 maintains ionic balance and supports normal cardiac rhythm making it essential for the contraction of the heart. Its expression and function can be influenced by interacting proteins and intracellular signaling molecules.

Pathways

Nav1.5 is an important component in the cardiac action potential generation and conduction pathways. It directly influences the cardiac conduction system and is part of the pathway that encompasses the sodium-potassium pump and other ion channels like Nav1.1 and Nav1.8. Through these pathways Nav1.5 regulates cardiac rhythm and myocardial contractility ensuring efficient heart function.

Nav1.5 mutations have significant associations with arrhythmias and cardiac conduction issues such as long QT syndrome and Brugada syndrome. These cardiac conditions are linked to disrupted sodium channel function caused by mutations in the SCN5A gene. Nav1.1 and Nav1.2 other family members of the sodium channel can sometimes show altered expression in these disorders reflecting the interconnected nature of this protein family in cardiac health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of Nav1.5, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed : 1309946, PubMed : 21447824, PubMed : 23085483, PubMed : 23420830, PubMed : 25370050, PubMed : 26279430, PubMed : 26392562, PubMed : 26776555). Nav1.5 is the predominant sodium channel expressed in myocardial cells and it is responsible for the initial upstroke of the action potential in cardiac myocytes, thereby initiating the heartbeat (PubMed : 11234013, PubMed : 11804990, PubMed : 12569159, PubMed : 1309946). Required for normal electrical conduction including formation of the infranodal ventricular conduction system and normal action potential configuration, as a result of its interaction with XIRP2 (By similarity).
See full target information SCN5A
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