AB65166

Anti-Nav1.6/SCN8A antibody

Name: Anti-Nav1.6/SCN8A antibody (ab65166) | Abcam
Brand: Abcam Limited
SKU: AB65166
Rating: 5 (4 reviews)

(4 Reviews)

(16 Publications)

Rabbit Polyclonal SCN8A antibody. Suitable for WB, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr, IHC-FrFl and reacts with Mouse, Rat, Human samples. Cited in 16 publications.

View Alternative Names

MED, SCN8A, Sodium channel protein type 8 subunit alpha, Sodium channel protein type VIII subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.6

1 Images

CiteAb

Western blot - Anti-Nav1.6/SCN8A antibody (AB65166)

Western Blotting using Anti-Nav1.6/SCN8A antibody, ab65166. Publication image from Arechederra, M. et al., 2018, Nat Commun, 30089774. Legend direct from paper.

Downregulation of overexpressed genes with hypermethylated gene body CGI in Alb-R26Met HCC cells interferes with their tumorigenic properties both in vitro and in vivo. a Western blots showing SCN8A, ACTN1, SRD5A2, NFkB2 and NEURL1B protein levels in stable clones established after transfection of Alb-R26Met HCC14 cells with plasmids carrying a shRNA sequence targeting the corresponding gene. Protein levels were compared to control cells (ctr). ACTIN was used as a loading control in all western blots. The asterisk indicates nonspecific bands detected using anti-SRD5A2 antibodies. b–e Biological assays to assess functional properties of Alb-R26Met HCC14 cells carrying a shRNA sequence targeting candidate genes. Effects were compared to HCC14 cells either untransfected or transfected with a control shRNA (shCtrl). b Graph reporting the number of colonies formed in anchorage-independent growth assays using 2 different shRNA targeting sequences for each candidate gene. Note a decrease in colony number formation of cells with downregulated candidate genes compared with control cells. c Graphs reporting the number (left) and the size (right) of colonies formed in anchorage-dependent growth assays. Whereas no significant changes in colony numbers were detected, note a significant decrease in colony size when the candidate gene is downregulated. d Graph reporting number of spheres formed in tumour sphere assays. Note that downregulation of candidate genes significantly reduces sphere number formation. e Graph reporting the tumour volume of mice injected either with Alb-R26Met HCC14 control cells or with Alb-R26Met HCC14 cells carrying a shRNA sequence targeting candidate genes. Note that downregulation of candidate genes significantly interferes with the in vivo tumorigenic properties of Alb-R26Met HCC14 cells. Significant differences between groups are indicated on the top. *P < 0.05, **P < 0.01, ***P < 0.001 (nd : no determined)

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, IHC-FrFl, IHC-P, ICC/IF, IHC-FoFr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nav1.6 also known as SCN8A is a voltage-gated sodium channel with a molecular mass of approximately 260 kDa. It is well-known for its significant role in propagating action potentials in neurons. Nav1.6 is highly expressed in the central nervous system specifically in the nodes of Ranvier and axon initial segments of neurons. This channel allows the influx of sodium ions which is critical for the rapid depolarization phase of action potentials facilitating nerve signal transmission.

Biological function summary

Nav1.6 impacts neuronal excitability and firing by controlling sodium ion flow across the nerve cell membrane. It forms an integral part of the channel complex that ensures proper electrical signaling within neurons. It largely contributes to high-frequency firing and sustained repetitive firing of specific neurons which is essential for normal brain function. Alterations in Nav1.6 function can lead to abnormal electrical activity in the nervous system impacting overall neuronal communication.

Pathways

Nav1.6 participates significantly in the action potential propagation pathway. It plays a role alongside other sodium channels such as Nav1.1 and Nav1.2 to maintain the intricate balance of excitatory signals. Nav1.6 is also involved in signaling cascades that affect synaptic transmission and plasticity. In these pathways Nav1.6 interacts indirectly with various ion channels and proteins which collectively modulate neuronal excitability and plasticity important for processes like learning and memory.

Disrupted Nav1.6 function associates with epileptic encephalopathy and ataxia. Mutations in the SCN8A gene that encodes for Nav1.6 can cause abnormal channel activity leading to hyperexcitability of neurons and seizure disorders. Additionally the improper functioning of Nav1.6 links to disorders featuring motor and cognitive impairments. In these contexts Nav1.6 is commonly discussed with proteins like Nav1.2 which also contribute to pathological neuronal excitability highlighting the interconnectedness of ion channel dynamics in neurological diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming subunit of a voltage-gated sodium channel complex assuming opened or closed conformations in response to the voltage difference across membranes and through which sodium ions selectively pass along their electrochemical gradient (PubMed : 24874546, PubMed : 25239001, PubMed : 25725044, PubMed : 26900580, PubMed : 29726066, PubMed : 33245860, PubMed : 36696443, PubMed : 36823201). Contributes to neuronal excitability by regulating action potential threshold and propagation (PubMed : 24874546, PubMed : 25239001, PubMed : 25725044, PubMed : 26900580, PubMed : 29726066, PubMed : 33245860, PubMed : 36696443, PubMed : 36823201).. Isoform 5. More specifically expressed in non-neuronal cells, could play a role in sodium release from intracellular compartments and participate in the control of podosomes formation and macrophages adhesion and movement.

See full target information SCN8A

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:105264 PubMed40697228

2025

Nav1.6 drives colorectal cancer proliferation and invasion through MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ming Zhao,Wan-Ying Hong,Jian-Guang Xu,Shui-Quan Lin,Ming-Sheng Liu,Li-Hui Wang,Xu-Li Jiang,Ming Sang,Yang-Bo Lv

British journal of pharmacology 182:2542-2559 PubMed39965632

2025

TREM2 activation reduces white matter injury via PI3K/Akt/GSK-3β signalling after intracerebral haemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,You Shi,Lin Wang,Zhao Li,Yingwen Wang,Jin Yan,Xiaochuan Sun,Qing Luo,Lin Li

The Journal of clinical investigation 134: PubMed39088270

2024

Exosomal TNF-α mediates voltage-gated Na+ channel 1.6 overexpression and contributes to brain tumor-induced neuronal hyperexcitability.

Applications

Unspecified application

Species

Unspecified reactive species

Cesar Adolfo Sanchez Trivino,Renza Spelat,Federica Spada,Camilla D'Angelo,Ivana Manini,Irene Giulia Rolle,Tamara Ius,Pietro Parisse,Anna Menini,Daniela Cesselli,Miran Skrap,Fabrizia Cesca,Vincent Torre

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2205862 PubMed36922751

2023

AQP4 Aggravates Cognitive Impairment in Sepsis-Associated Encephalopathy through Inhibiting Na 1.6-Mediated Astrocyte Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Zhu,Yue-Lin Huang,Song-Yu Guo,Na Li,Xue-Wei Yang,Ao-Ran Sui,Qiong Wu,Yue Zhang,Yue Kong,Qi-Fa Li,Ting Zhang,Wen-Fei Zheng,Ai-Ping Li,Jian Yu,Tong-Hui Ma,Shao Li

Frontiers in molecular neuroscience 15:822129 PubMed35557557

2022

Social Deficits and Cerebellar Degeneration in Purkinje Cell Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofan Yang,Hongqiang Yin,Xiaojing Wang,Yueqing Sun,Xianli Bian,Gaorui Zhang,Anning Li,Aihua Cao,Baomin Li,Darius Ebrahimi-Fakhari,Zhuo Yang,Miriam H Meisler,Qiji Liu

Science advances 7: PubMed33741588

2021

Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Okada,Daisuke Kato,Yuki Nomura,Norihiko Obata,Xiangyu Quan,Akihito Morinaga,Hajime Yano,Zhongtian Guo,Yuki Aoyama,Yoshihisa Tachibana,Andrew J Moorhouse,Osamu Matoba,Tetsuya Takiguchi,Satoshi Mizobuchi,Hiroaki Wake

Brain research bulletin 170:187-198 PubMed33610724

2021

Altered expression of voltage gated sodium channel Nav1.1 is involved in motor ability in MPTP-treated mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weitang Liu,Wenwen Lao,Renxing Zhang,Hongyan Zhu

Marine drugs 18: PubMed32471037

2020

Neosaxitoxin Inhibits the Expression of Inflammation Markers of the M1 Phenotype in Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

M Cecilia Montero,Miguel Del Campo,M Bono,M Valeska Simon,Julia Guerrero,Néstor Lagos

Neural plasticity 2020:9132720 PubMed32256560

2020

Exercise-Induced Cognitive Improvement Is Associated with Sodium Channel-Mediated Excitability in APP/PS1 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Xin Tan,Guang-Cai Liu,Hong-Lan Chen,Min-Nan Lu,Bo Chen,Tao Hu,Li Zhang,Rui Mao,Shan Li,Rong Mei,Xu-Yang Wang,Yan-Bin Xiyang

Neuroscience 418:231-243 PubMed31473280

2019

Voltage-Gated Sodium Channels Are Involved in Cognitive Impairments in Parkinson's Disease- like Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Wang,Yuying Lin,Weitang Liu,Ping Kuang,Wenwen Lao,Yonghua Ji,Hongyan Zhu

View all publications

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Product promise

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Anti-Nav1.6/SCN8A antibody

Western blot - Anti-Nav1.6/SCN8A antibody (AB65166)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise