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Rabbit Recombinant Monoclonal SCN8A antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786), expandable thumbnail
  • Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow Cyt (Intra)IP
Human
Tested
Not recommended
Expected
Not recommended
Not recommended
Expected
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Tested
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Rat
Dilution info
1/30
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Pore-forming subunit of a voltage-gated sodium channel complex assuming opened or closed conformations in response to the voltage difference across membranes and through which sodium ions selectively pass along their electrochemical gradient (PubMed:24874546, PubMed:25239001, PubMed:25725044, PubMed:26900580, PubMed:29726066, PubMed:33245860, PubMed:36696443, PubMed:36823201). Contributes to neuronal excitability by regulating action potential threshold and propagation (PubMed:24874546, PubMed:25239001, PubMed:25725044, PubMed:26900580, PubMed:29726066, PubMed:33245860, PubMed:36696443, PubMed:36823201). Isoform 5. More specifically expressed in non-neuronal cells, could play a role in sodium release from intracellular compartments and participate in the control of podosomes formation and macrophages adhesion and movement.

Alternative names

MED, SCN8A, Sodium channel protein type 8 subunit alpha, Sodium channel protein type VIII subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.6

Recommended products

Rabbit Recombinant Monoclonal SCN8A antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25137-45
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nav1.6 also known as SCN8A is a voltage-gated sodium channel with a molecular mass of approximately 260 kDa. It is well-known for its significant role in propagating action potentials in neurons. Nav1.6 is highly expressed in the central nervous system specifically in the nodes of Ranvier and axon initial segments of neurons. This channel allows the influx of sodium ions which is critical for the rapid depolarization phase of action potentials facilitating nerve signal transmission.

Biological function summary

Nav1.6 impacts neuronal excitability and firing by controlling sodium ion flow across the nerve cell membrane. It forms an integral part of the channel complex that ensures proper electrical signaling within neurons. It largely contributes to high-frequency firing and sustained repetitive firing of specific neurons which is essential for normal brain function. Alterations in Nav1.6 function can lead to abnormal electrical activity in the nervous system impacting overall neuronal communication.

Pathways

Nav1.6 participates significantly in the action potential propagation pathway. It plays a role alongside other sodium channels such as Nav1.1 and Nav1.2 to maintain the intricate balance of excitatory signals. Nav1.6 is also involved in signaling cascades that affect synaptic transmission and plasticity. In these pathways Nav1.6 interacts indirectly with various ion channels and proteins which collectively modulate neuronal excitability and plasticity important for processes like learning and memory.

Associated diseases and disorders

Disrupted Nav1.6 function associates with epileptic encephalopathy and ataxia. Mutations in the SCN8A gene that encodes for Nav1.6 can cause abnormal channel activity leading to hyperexcitability of neurons and seizure disorders. Additionally the improper functioning of Nav1.6 links to disorders featuring motor and cognitive impairments. In these contexts Nav1.6 is commonly discussed with proteins like Nav1.2 which also contribute to pathological neuronal excitability highlighting the interconnectedness of ion channel dynamics in neurological diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on rat liver (PMID: 11532991) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on mouse liver (PMID: 11532991) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

  • Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786), expandable thumbnail

    Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 (0.246 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Negative control: No staining on rat liver. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786), expandable thumbnail

    Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 (0.246 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Negative control: No staining on mouse liver. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786), expandable thumbnail

    Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 (0.246 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Positive staining on axon in rat cerebrum. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786), expandable thumbnail

    Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 (0.246 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Positive staining on axon in mouse cerebrum. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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Product protocols

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