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AB302786

Anti-Nav1.6/SCN8A antibody [EPR25137-45]

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(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal SCN8A antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

MED, SCN8A, Sodium channel protein type 8 subunit alpha, Sodium channel protein type VIII subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.6

9 Images
Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • IHC

Supplier Data

Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 (0.246 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Positive staining on axon in rat cerebrum. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • IHC

Supplier Data

Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 (0.246 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Negative control : No staining on mouse liver. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • IHC

Supplier Data

Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 (0.246 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Positive staining on axon in mouse cerebrum. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • IHC

Supplier Data

Immunohistochemistry - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Nav1.6/SCN8A with ab302786 at 1/2000 (0.246 ug/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Negative control : No staining on rat liver. The section was incubated with ab302786 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) at Ready to use dilution. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on rat liver (PMID : 11532991) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on mouse liver (PMID : 11532991) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Nav1.6/SCN8A with ab302786 at 1/500 (0.982 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .

Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)
  • WB

Supplier Data

Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (AB302786)

Blocking and dilution buffer : 5% NFDM/TBST.

The expression molecular weight observed is consistent with what has been described in the literature (PMID : 26410685).

Negative control : Liver (PMID : 11532991).

Bands around 150 kDa and 250 kDa may due to degradation.

Samples are non-boiled as boiling may cause protein aggregates.

Lane 5-7 of the blot was developed using a high sensitivity ECL substrate.

Exposure time : Lane 1-4 : 114 seconds, Lane 5-7 : 48 seconds.

All lanes:

Western blot - Anti-Nav1.6/SCN8A antibody [EPR25137-45] (ab302786) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat spinal cord tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Lane 5:

Human spinal cord tissue lysate at 20 µg

Lane 6:

Human cerebellum tissue lysate at 20 µg

Lane 7:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 260 kDa

false

  • Carrier free

    Anti-Nav1.6/SCN8A antibody [EPR25137-45] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25137-45

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, IHC-Fr, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nav1.6 also known as SCN8A is a voltage-gated sodium channel with a molecular mass of approximately 260 kDa. It is well-known for its significant role in propagating action potentials in neurons. Nav1.6 is highly expressed in the central nervous system specifically in the nodes of Ranvier and axon initial segments of neurons. This channel allows the influx of sodium ions which is critical for the rapid depolarization phase of action potentials facilitating nerve signal transmission.
Biological function summary

Nav1.6 impacts neuronal excitability and firing by controlling sodium ion flow across the nerve cell membrane. It forms an integral part of the channel complex that ensures proper electrical signaling within neurons. It largely contributes to high-frequency firing and sustained repetitive firing of specific neurons which is essential for normal brain function. Alterations in Nav1.6 function can lead to abnormal electrical activity in the nervous system impacting overall neuronal communication.

Pathways

Nav1.6 participates significantly in the action potential propagation pathway. It plays a role alongside other sodium channels such as Nav1.1 and Nav1.2 to maintain the intricate balance of excitatory signals. Nav1.6 is also involved in signaling cascades that affect synaptic transmission and plasticity. In these pathways Nav1.6 interacts indirectly with various ion channels and proteins which collectively modulate neuronal excitability and plasticity important for processes like learning and memory.

Disrupted Nav1.6 function associates with epileptic encephalopathy and ataxia. Mutations in the SCN8A gene that encodes for Nav1.6 can cause abnormal channel activity leading to hyperexcitability of neurons and seizure disorders. Additionally the improper functioning of Nav1.6 links to disorders featuring motor and cognitive impairments. In these contexts Nav1.6 is commonly discussed with proteins like Nav1.2 which also contribute to pathological neuronal excitability highlighting the interconnectedness of ion channel dynamics in neurological diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of a voltage-gated sodium channel complex assuming opened or closed conformations in response to the voltage difference across membranes and through which sodium ions selectively pass along their electrochemical gradient (PubMed : 24874546, PubMed : 25239001, PubMed : 25725044, PubMed : 26900580, PubMed : 29726066, PubMed : 33245860, PubMed : 36696443, PubMed : 36823201). Contributes to neuronal excitability by regulating action potential threshold and propagation (PubMed : 24874546, PubMed : 25239001, PubMed : 25725044, PubMed : 26900580, PubMed : 29726066, PubMed : 33245860, PubMed : 36696443, PubMed : 36823201).. Isoform 5. More specifically expressed in non-neuronal cells, could play a role in sodium release from intracellular compartments and participate in the control of podosomes formation and macrophages adhesion and movement.
See full target information SCN8A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1542015 PubMed40160459

2025

The potential role and mechanism of Rhizoma Coptidis in prevention of diabetic encephalopathy: targeting sodium ion and channels.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Cao,Zhangxuan Shou,Mimi Wang,You Wu,Xuefeng Wang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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