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Rabbit Recombinant Monoclonal SCN9A antibody. Suitable for IHC-Fr, IHC-P, WB, Dot and reacts with Mouse, Rat, Transfected cell lysate - Mouse samples.

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Images

Dot Blot - Anti-Nav1.7 antibody [EPR26246-318] (AB323849), expandable thumbnail
  • Western blot - Anti-Nav1.7 antibody [EPR26246-318] (AB323849), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrIHC-PWBDotICC/IF
Mouse
Tested
Tested
Tested
Expected
Not recommended
Rat
Tested
Tested
Tested
Expected
Not recommended
Transfected cell lysate - Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Target data

Function

Pore-forming subunit of Nav1.7, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (By similarity). Nav1.7 plays a crucial role in controlling the excitability and action potential propagation from nociceptor neurons, thereby contributing to the sensory perception of pain (PubMed:15314237).

Alternative names

Kiaa4197, Scn9a, Sodium channel protein type 9 subunit alpha, Peripheral sodium channel 1, Sodium channel protein type IX subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.7, PN1

Rabbit Recombinant Monoclonal SCN9A antibody. Suitable for IHC-Fr, IHC-P, WB, Dot and reacts with Mouse, Rat, Transfected cell lysate - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26246-318
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Dot Blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Dot Blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Dot Blot staining using rabbit Anti-Nav1.7 antibody

    Dot blot analysis of Nav1.7 using ab323849 at 1:1000 (0.493 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: 293T cells transfected with a mouse SCN2A expression vector containing a His-tag, whole cell lysate

    Lane2: 293T cells transfected with a mouse SCN3A expression vector containing a His-tag, whole cell lysate

    Lane3: 293T cells transfected with a mouse SCN1A expression vector containing a His-tag, whole cell lysate

    Lane4: 293T cells transfected with a mouse SCN9A expression vector containing a His-tag, whole cell lysate

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with mouse SCN1A, SCN2A and SCN3A.

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849) at 1/1000 dilution

    Lane 1: 293T cells transfected with a mouse SCN2A expression vector containing a His-tag, whole cell lysate

    Lane 2: 293T cells transfected with a mouse SCN3A expression vector containing a His-tag, whole cell lysate

    Lane 3: 293T cells transfected with a mouse SCN1A expression vector containing a His-tag, whole cell lysate

    Lane 4: 293T cells transfected with a mouse SCN9A expression vector containing a His-tag, whole cell lysate

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Western blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Western blot staining using rabbit Anti-Nav1.7 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: skeletal muscle (PMID: 9037087).

    Samples are non-boiled as boiling may cause protein aggregation.

    In lanes 1-2, to minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    All lanes: Western blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849) at 1/1000 dilution

    Lane 1: Mouse skeletal muscle tissue lysate(Fresh) at 40 µg

    Lane 2: Mouse sciatic nerve tissue lysate(Fresh) at 40 µg

    Lane 3: Mouse skeletal muscle tissue lysate(Frozen) at 50 µg

    Lane 4: Mouse sciatic nerve tissue lysate(Frozen) at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 270 kDa, 124 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse skeletal muscle using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on mouse skeletal muscle (PMID: 9037087).

    The section was incubated with ab323849 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat spinal cord using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of paraffin-embedded Rat spinal cord tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat spinal cord.

    The section was incubated with ab323849 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Frozen sections) staining of Rat olfactory bulb (fresh frozen) using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat olfactory bulb (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Panel A: merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] ab311892, magenta) on rat olfactory bulb.

    Panel B: anti-SCN9A stained on rat olfactory bulb.

    Panel C: anti-Peripherin stained in neurons of rat olfactory bulb.

    The section was incubated in two rounds of staining: in the order of ab323849 and Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Frozen sections) staining of Mouse olfactory bulb (fresh frozen) using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse olfactory bulb (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Panel A: merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] ab311892, magenta) on mouse olfactory bulb.

    Panel B: anti-SCN9A stained on mouse olfactory bulb.

    Panel C: anti-Peripherin stained in neurons of mouse olfactory bulb.

    The section was incubated in two rounds of staining: in the order of ab323849 and Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Western blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Western blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Western blot staining using rabbit Anti-Nav1.7 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: skeletal muscle (PMID: 9037087).

    Samples are non-boiled as boiling may cause protein aggregation.

    To minimize protein degradation, lane1-3 were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    Exposure time: Lane 1: 136 seconds, Lanes 2-3: 10 seconds, Lane 4: 180 seconds

    All lanes: Western blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849) at 1/1000 dilution

    Lane 1: Rat spinal cord tissue lysate(Fresh) at 80 µg

    Lane 2: Rat olfactory bulb tissue lysate(Fresh) at 80 µg

    Lane 3: Rat skeletal muscle tissue lysate(Frozen) at 80 µg

    Lane 4: Rat olfactory bulb tissue lysate(Frozen) at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 270 kDa, 124 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse olfactory bulb using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse olfactory bulb tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse olfactory bulb.

    The section was incubated with ab323849 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Frozen sections) staining of Mouse skeletal muscle (fresh frozen) using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Negative control: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323849 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Frozen sections) staining of Rat skeletal muscle (fresh frozen) using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Negative control: confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323849 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat skeletal muscle using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on rat skeletal muscle.

    The section was incubated with ab323849 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat olfactory bulb using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of paraffin-embedded Rat olfactory bulb tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat olfactory bulb.

    The section was incubated with ab323849 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Frozen sections) staining of Rat sciatic nerve (fresh frozen) using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat sciatic nerve (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Panel A: merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] ab311892, magenta) on rat sciatic nerve.

    Panel B: anti-SCN9A stained on rat sciatic nerve.

    Panel C: anti-Peripherin stained on rat sciatic nerve.

    The section was incubated in two rounds of staining: in the order of ab323849 and Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Frozen sections) staining of Mouse sciatic nerve (fresh frozen) using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse sciatic nerve (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Panel A: merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] ab311892, magenta) on mouse sciatic nerve.

    Panel B: anti-SCN9A stained on mouse sciatic nerve.

    Panel C: anti-Peripherin stained on mouse sciatic nerve.

    The section was incubated in two rounds of staining: in the order of ab323849 and Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (ab323849)

    Nav1.7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spinal cord using rabbit Anti-Nav1.7 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spinal cord.

    The section was incubated with ab323849 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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