Anti-Nav1.7 antibody [EPR26246-318]

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse olfactory bulb (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (ab311892, magenta) on mouse olfactory bulb.
Panel B : anti-SCN9A stained on mouse olfactory bulb.
Panel C : anti-Peripherin stained in neurons of mouse olfactory bulb.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of paraffin-embedded Rat olfactory bulb tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat olfactory bulb.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : skeletal muscle (PMID : 9037087).

Samples are non-boiled as boiling may cause protein aggregation.

In lanes 1-2, to minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Western blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate(Fresh) at 40 µg

Lane 2:

Mouse sciatic nerve tissue lysate(Fresh) at 40 µg

Lane 3:

Mouse skeletal muscle tissue lysate(Frozen) at 50 µg

Lane 4:

Mouse sciatic nerve tissue lysate(Frozen) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Dot blot analysis of Nav1.7 using ab323849 at 1 : 1000 (0.493 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : 293T cells transfected with a mouse SCN2A expression vector containing a His-tag, whole cell lysate
Lane2 : 293T cells transfected with a mouse SCN3A expression vector containing a His-tag, whole cell lysate
Lane3 : 293T cells transfected with a mouse SCN1A expression vector containing a His-tag, whole cell lysate
Lane4 : 293T cells transfected with a mouse SCN9A expression vector containing a His-tag, whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse SCN1A, SCN2A and SCN3A.

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse SCN2A expression vector containing a His-tag, whole cell lysate

Lane 2:

293T cells transfected with a mouse SCN3A expression vector containing a His-tag, whole cell lysate

Lane 3:

293T cells transfected with a mouse SCN1A expression vector containing a His-tag, whole cell lysate

Lane 4:

293T cells transfected with a mouse SCN9A expression vector containing a His-tag, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Negative control : confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323849 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat sciatic nerve (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (ab311892, magenta) on rat sciatic nerve.
Panel B : anti-SCN9A stained on rat sciatic nerve.
Panel C : anti-Peripherin stained on rat sciatic nerve.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat olfactory bulb (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (ab311892, magenta) on rat olfactory bulb.
Panel B : anti-SCN9A stained on rat olfactory bulb.
Panel C : anti-Peripherin stained in neurons of rat olfactory bulb.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Negative control : confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323849 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse sciatic nerve (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849, green) and anti-Peripherin (ab311892, magenta) on mouse sciatic nerve.
Panel B : anti-SCN9A stained on mouse sciatic nerve.
Panel C : anti-Peripherin stained on mouse sciatic nerve.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat skeletal muscle.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse skeletal muscle (PMID : 9037087).
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of paraffin-embedded Rat spinal cord tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat spinal cord.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spinal cord.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Immunohistochemical analysis of paraffin-embedded Mouse olfactory bulb tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse olfactory bulb.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Nav1.7 antibody [EPR26246-318] (AB323849)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : skeletal muscle (PMID : 9037087).

Samples are non-boiled as boiling may cause protein aggregation.

To minimize protein degradation, lane1-3 were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

Exposure time : Lane 1 : 136 seconds, Lanes 2-3 : 10 seconds, Lane 4 : 180 seconds

All lanes:

Western blot - Anti-Nav1.7 antibody [EPR26246-318] (ab323849) at 1/1000 dilution

Lane 1:

Rat spinal cord tissue lysate(Fresh) at 80 µg

Lane 2:

Rat olfactory bulb tissue lysate(Fresh) at 80 µg

Lane 3:

Rat skeletal muscle tissue lysate(Frozen) at 80 µg

Lane 4:

Rat olfactory bulb tissue lysate(Frozen) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

false