AB325967

Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SCN9A antibody. Carrier free. Suitable for IHC-Fr, IHC-P, WB, Dot and reacts with Mouse, Rat, Transfected cell line - Mouse samples.

View Alternative Names

Kiaa4197, Scn9a, Sodium channel protein type 9 subunit alpha, Peripheral sodium channel 1, Sodium channel protein type IX subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.7, PN1

15 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spinal cord.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse olfactory bulb tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse olfactory bulb.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Negative control : confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323849 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Rat sciatic nerve (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849 green) and anti-Peripherin (ab311892 magenta) on rat sciatic nerve.
Panel B : anti-SCN9A stained on rat sciatic nerve.
Panel C : anti-Peripherin stained on rat sciatic nerve.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Rat olfactory bulb (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849 green) and anti-Peripherin (ab311892 magenta) on rat olfactory bulb.
Panel B : anti-SCN9A stained on rat olfactory bulb.
Panel C : anti-Peripherin stained in neurons of rat olfactory bulb.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Negative control : confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab323849 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Mouse sciatic nerve (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849 green) and anti-Peripherin (ab311892 magenta) on mouse sciatic nerve.
Panel B : anti-SCN9A stained on mouse sciatic nerve.
Panel C : anti-Peripherin stained on mouse sciatic nerve.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Mouse olfactory bulb (fresh frozen) tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-SCN9A (ab323849 green) and anti-Peripherin (ab311892 magenta) on mouse olfactory bulb.
Panel B : anti-SCN9A stained on mouse olfactory bulb.
Panel C : anti-Peripherin stained in neurons of mouse olfactory bulb.
The section was incubated in two rounds of staining : in the order of ab323849 and ab311892 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat skeletal muscle.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse skeletal muscle (PMID : 9037087).
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spinal cord tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat spinal cord.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat olfactory bulb tissue labeling Nav1.7 with ab323849 at 1/100 (4.93 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat olfactory bulb.
The section was incubated with ab323849 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : skeletal muscle (PMID : 9037087).

Samples are non-boiled as boiling may cause protein aggregation.

To minimize protein degradation lane1-3 were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

Exposure time : Lane 1 : 136 seconds Lanes 2-3 : 10 seconds Lane 4 : 180 seconds

All lanes:

Western blot - Anti-Nav1.7 antibody [EPR26246-318] (<a href='/en-us/products/primary-antibodies/nav17-antibody-epr26246-318-ab323849'>ab323849</a>) at 1/1000 dilution

Lane 1:

Rat spinal cord tissue lysate(Fresh) at 80 µg

Lane 2:

Rat olfactory bulb tissue lysate(Fresh) at 80 µg

Lane 3:

Rat skeletal muscle tissue lysate(Frozen) at 80 µg

Lane 4:

Rat olfactory bulb tissue lysate(Frozen) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

false

Supplier Data

Western blot - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : skeletal muscle (PMID : 9037087).

Samples are non-boiled as boiling may cause protein aggregation.

In lanes 1-2 to minimize protein degradation tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Western blot - Anti-Nav1.7 antibody [EPR26246-318] (<a href='/en-us/products/primary-antibodies/nav17-antibody-epr26246-318-ab323849'>ab323849</a>) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate(Fresh) at 40 µg

Lane 2:

Mouse sciatic nerve tissue lysate(Fresh) at 40 µg

Lane 3:

Mouse skeletal muscle tissue lysate(Frozen) at 50 µg

Lane 4:

Mouse sciatic nerve tissue lysate(Frozen) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-Nav1.7 antibody [EPR26246-318] - BSA and Azide free (AB325967)

This data was developed using ab323849, the same antibody clone in a different buffer formulation.

Dot blot analysis of Nav1.7 using ab323849 at 1 : 1000 (0.493 ug/ml) followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1 : 100000 dilution.

Lane1 : 293T cells transfected with a mouse SCN2A expression vector containing a His-tag whole cell lysate
Lane2 : 293T cells transfected with a mouse SCN3A expression vector containing a His-tag whole cell lysate
Lane3 : 293T cells transfected with a mouse SCN1A expression vector containing a His-tag whole cell lysate
Lane4 : 293T cells transfected with a mouse SCN9A expression vector containing a His-tag whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse SCN1A SCN2A and SCN3A.

In Dot Blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-Nav1.7 antibody [EPR26246-318] (<a href='/en-us/products/primary-antibodies/nav17-antibody-epr26246-318-ab323849'>ab323849</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse SCN2A expression vector containing a His-tag, whole cell lysate

Lane 2:

293T cells transfected with a mouse SCN3A expression vector containing a His-tag, whole cell lysate

Lane 3:

293T cells transfected with a mouse SCN1A expression vector containing a His-tag, whole cell lysate

Lane 4:

293T cells transfected with a mouse SCN9A expression vector containing a His-tag, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26246-318

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, Dot, WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab325967 is the carrier-free version of ab323849

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming subunit of Nav1.7, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (By similarity). Nav1.7 plays a crucial role in controlling the excitability and action potential propagation from nociceptor neurons, thereby contributing to the sensory perception of pain (PubMed : 15314237).

See full target information Scn9a

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com