AB85015

Anti-Nav1.7 antibody [N68/6]

Name: Anti-Nav1.7 antibody [N68/6] (ab85015) | Abcam
Brand: Abcam Limited
SKU: AB85015
Price: 435 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(15 Publications)

Mouse Monoclonal SCN9A antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 15 publications.

View Alternative Names

NENA, SCN9A, Sodium channel protein type 9 subunit alpha, Neuroendocrine sodium channel, Peripheral sodium channel 1, Sodium channel protein type IX subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.7, hNE-Na, PN1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [N68/6] (AB85015)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [N68/6] (AB85015)

ab85015 at 1/100 dilution staining Nav1.7 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used. Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N68/6

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Some customers have used this antibody successfully in rat, however our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

The clone number has been updated from S68-6 to N68/6, both clone numbers name the same antibody clone.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nav1.7 also known as SCN9A is a voltage-gated sodium channel. This protein has a mass of approximately 260 kDa. It plays a role in the generation and conduction of action potentials in neurons. Nav1.7 is found mainly in peripheral neurons including dorsal root ganglia and sympathetic ganglia. These neurons are part of the sensory pathways responsible for transmitting pain signals.

Biological function summary

Nav1.7 acts as an essential component in neuronal excitability. It is not part of a larger protein complex but operates as an individual unit within the cell membrane. By allowing sodium ions to enter the neuron Nav1.7 contributes to the depolarization phase of action potentials. This function is critical in the propagation of electrical signals in the nervous system specifically in nociceptive neurons that are involved in pain sensation.

Pathways

Nav1.7 is integral to the nociceptive pain pathway. It works with other sodium channels such as Nav1.8 which also play roles in pain sensation. Nav1.7's activity helps to initiate and maintain action potentials facilitating the transmission of pain signals to the central nervous system. Additionally Nav1.7 is linked to neuropathic pain pathways where its function underpins the continuous firing of neurons even in the absence of external stimuli.

Nav1.7 mutations have a significant association with primary erythromelalgia and congenital indifference to pain. Mutations that cause gain-of-function in Nav1.7 can lead to primary erythromelalgia resulting in extreme pain and redness in the body’s extremities. Conversely loss-of-function mutations can cause congenital indifference to pain where individuals experience little to no pain sensation. Nav1.7 works alongside Nav1.8 in these conditions as both are involved in the modulation of pain signals.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming subunit of Nav1.7, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed : 15385606, PubMed : 16988069, PubMed : 17145499, PubMed : 17167479, PubMed : 19369487, PubMed : 24311784, PubMed : 25240195, PubMed : 26680203, PubMed : 7720699). Nav1.7 plays a crucial role in controlling the excitability and action potential propagation from nociceptor neurons, thereby contributing to the sensory perception of pain (PubMed : 17145499, PubMed : 17167479, PubMed : 19369487, PubMed : 24311784).

See full target information SCN9A

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37345144

2023

Voltage-Gated Sodium Channel Na1.7 Inhibitors with Potent Anticancer Activities in Medullary Thyroid Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Piyasuda Pukkanasut,Jason Whitt,Rachael Guenter,Shannon E Lynch,Carlos Gallegos,Margarita Jacaranda Rosendo-Pineda,Juan Carlos Gomora,Herbert Chen,Diana Lin,Anna Sorace,Renata Jaskula-Sztul,Sadanandan E Velu

Frontiers in molecular neuroscience 16:1091096 PubMed36937049

2023

Ectopic expression of Nav1.7 in spinal dorsal horn neurons induced by NGF contributes to neuropathic pain in a mouse spinal cord injury model.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Fu,Liting Sun,Fengting Zhu,Wei Xia,Ting Wen,Ruilong Xia,Xin Yu,Dan Xu,Changgeng Peng

Frontiers in cellular neuroscience 16:1039957 PubMed36733665

2023

Bringing to light the physiological and pathological firing patterns of human induced pluripotent stem cell-derived neurons using optical recordings.

Applications

Unspecified application

Species

Unspecified reactive species

Therese C Alich,Pascal Röderer,Balint Szalontai,Kurt Golcuk,Shahan Tariq,Michael Peitz,Oliver Brüstle,Istvan Mody

Pain 164:1718-1733 PubMed36727909

2023

Emergence of nociceptive functionality and opioid signaling in human induced pluripotent stem cell-derived sensory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Pascal Röderer,Andreea Belu,Luzia Heidrich,Maike Siobal,Jörg Isensee,Jonathan Prolingheuer,Elke Janocha,Markus Valdor,Silke Hagendorf,Gregor Bahrenberg,Thoralf Opitz,Michaela Segschneider,Simone Haupt,Anja Nitzsche,Oliver Brüstle,Tim Hucho

Neurobiology of pain (Cambridge, Mass.) 12:100109 PubMed36531612

2022

Cell specific regulation of NaV1.7 activity and trafficking in rat nodose ganglia neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Santiago I Loya-López,Paz Duran,Dongzhi Ran,Aida Calderon-Rivera,Kimberly Gomez,Aubin Moutal,Rajesh Khanna

Molecular pharmaceutics : PubMed33404254

2021

Bimodal Imaging of Mouse Peripheral Nerves with Chlorin Tracers.

Applications

Unspecified application

Species

Unspecified reactive species

Junior Gonzales,Javier Hernández-Gil,Thomas C Wilson,Dauren Adilbay,Mike Cornejo,Paula Demétrio de Souza Franca,Navjot Guru,Christina I Schroeder,Glenn F King,Jason S Lewis,Thomas Reiner

International journal of molecular medicine 46:782-794 PubMed32468069

2020

Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jie Tian,Chao-Yang Tan,Qin-Yi Chen,Ying Zhou,Zu-Wei Qu,Meng Zhang,Ke-Tao Ma,Wen-Yan Shi,Li Li,Jun-Qiang Si

EJNMMI research 10:49 PubMed32409881

2020

Fluorescence labeling of a Na1.7-targeted peptide for near-infrared nerve visualization.

Applications

Unspecified application

Species

Unspecified reactive species

Junior Gonzales,Giacomo Pirovano,Chun Yuen Chow,Paula Demetrio de Souza Franca,Lukas M Carter,Julie K Klint,Navjot Guru,Jason S Lewis,Glenn F King,Thomas Reiner

Channels (Austin, Tex.) 14:69-78 PubMed32089065

2020

Activating Sirt1 by resveratrol suppresses Nav1.7 expression in DRG through miR-182 and alleviates neuropathic pain in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Jia,Wenze Dong,Liwei Zhang,Xiaochun Yang

Bioconjugate chemistry 30:2879-2888 PubMed31647222

2019

Fluorescence Imaging of Peripheral Nerves by a Na1.7-Targeted Inhibitor Cystine Knot Peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Junior Gonzales,Paula Demetrio de Souza Franca,Yan Jiang,Giacomo Pirovano,Susanne Kossatz,Navjot Guru,Dimitry Yarilin,Akello J Agwa,Christina I Schroeder,Snehal G Patel,Ian Ganly,Glenn F King,Thomas Reiner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nav1.7 antibody [N68/6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [N68/6] (AB85015)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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