AB66743

Anti-Nav1.8/SCN10A antibody

Name: Anti-Nav1.8/SCN10A antibody (ab66743) | Abcam
Brand: Abcam Limited
SKU: AB66743

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(6 Publications)

Rabbit Polyclonal Nav1.8/SCN10A antibody. Suitable for ICC and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SCN10A.

View Alternative Names

Sodium channel protein type 10 subunit alpha, Peripheral nerve sodium channel 3, Sodium channel protein type X subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.8, PN3, hPN3, SCN10A

1 Images

Supplier Data

Immunocytochemistry - Anti-Nav1.8/SCN10A antibody (AB66743)

ab66743 staining Nav1.8/SCN10A in human sperm cells by Immunocytochemistry/ Immunofluorescence.Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab66743 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human SCN10A. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/1000", "ICC-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nav1.8 also known as SCN10A is a voltage-gated sodium channel. It facilitates the rapid transit of sodium ions across cell membranes essential for the propagation of action potentials in neurons. Nav1.8 has a mass of approximately 220 kDa and it expresses primarily in peripheral sensory neurons including dorsal root ganglia. This channel is activated mainly in response to depolarization enabling the initiation and conduction of electrical signals.

Biological function summary

Nav1.8 contributes significantly to pain transmission especially in nociceptive neurons which detect painful stimuli. It operates as a principal component in generating sustained action potentials required for pain signal propagation. Nav1.8 is not a part of a larger complex but plays a standalone critical role. It shows high expression during inflammatory and neuropathic pain conditions indicating its role in pain pathways.

Pathways

Nav1.8 is involved in both the pain and neuroinflammatory pathways. It influences the activity of other sodium channels such as Nav1.7 another critical mediator of pain sensation. Pain pathways extensively integrate Nav1.8 function especially in the modulation of pain thresholds and states. Neuroinflammatory pathways utilize Nav1.8 to alter neuron excitability under inflammatory conditions which is significant for understanding different pain modalities.

Nav1.8 is associated with chronic pain disorders such as neuropathic pain and inflammatory pain conditions. Variations in Nav1.8 are linked to altered pain sensitivity and are researched as potential therapeutic targets for pain relief. In diseases like diabetic neuropathy Nav1.8 changes correlate with increased neuronal excitability and persistent pain sensation. Its relationship with proteins like Nav1.7 in these contexts highlights its importance in the diagnostic and therapeutic options for pain management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.

See full target information SCN10A

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurochemistry 169:e70004 PubMed39871624

2025

GABA Receptor Modulation of Membrane Excitability in Human Pluripotent Stem Cell-Derived Sensory Neurons by Baclofen and α-Conotoxin Vc1.1.

Applications

Unspecified application

Species

Unspecified reactive species

Mitchell St Clair-Glover,Arsalan Yousuf,Dominic Kaul,Mirella Dottori,David J Adams

eNeuro 11: PubMed39542733

2024

Procaine Regulates the STAT3/CCL5 Axis and Inhibits Microglia M1 Polarization to Alleviate Complete Freund's Adjuvant Rats Pain Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Kai Zhang,Chen Li,QingDong Wang,Rongjia Zang

Frontiers in cellular neuroscience 14:600895 PubMed33362470

2020

Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Amy J Hulme,Jeffrey R McArthur,Simon Maksour,Sara Miellet,Lezanne Ooi,David J Adams,Rocio K Finol-Urdaneta,Mirella Dottori

The journal of headache and pain 15:63 PubMed25261281

2014

Expression of 5-HT3 receptors and TTX resistant sodium channels (Na(V)1.8) on muscle nerve fibers in pain-free humans and patients with chronic myofascial temporomandibular disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolaos Christidis,Isabell Kang,Brian E Cairns,Ujendra Kumar,Xudong Dong,Annika Rosén,Sigvard Kopp,Malin Ernberg

PloS one 8:e76084 PubMed24086692

2013

The voltage-gated sodium channel nav1.8 is expressed in human sperm.

Applications

ICC/IF, Flow Cyt

Species

Human, Human

Antonio Cejudo-Roman,Francisco M Pinto,Nerea Subirán,Cristina G Ravina,Manuel Fernández-Sánchez,Natalia Pérez-Hernández,Ricardo Pérez,Alberto Pacheco,Jon Irazusta,Luz Candenas

Reproductive biology and endocrinology : RB&E 7:71 PubMed19607678

2009

Molecular and functional characterization of voltage-gated sodium channels in human sperm.

Applications

ICC/IF

Species

Human

Francisco M Pinto,Cristina G Ravina,Manuel Fernández-Sánchez,Manuel Gallardo-Castro,Antonio Cejudo-Román,Luz Candenas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nav1.8/SCN10A antibody

Immunocytochemistry - Anti-Nav1.8/SCN10A antibody (AB66743)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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