Anti-Nav1.8/SCN10A antibody [EPR25132-222]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR25132-222] (AB307817)

Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglia tissue labeling Nav1.8/SCN10A with ab307817 at 1/2000 dilution (0.266 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat dorsal root ganglia. The section was incubated with ab307817 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [EPR25132-222] (AB307817)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary DRG neuron cells labelling Nav1.8/SCN10A with ab307817 at 1/100 (5.32 μg/ml) (shown in Green).

Confocal image showing cytoplasmic staining in rat primary DRG neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution, followed by secondary counterstain ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution.

Secondary ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was used at 1/1000 (2μg/ml)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR25132-222] (AB307817)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Nav1.8/SCN10A with ab307817 at 1/2000 dilution (0.266 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on rat liver. The section was incubated with ab307817 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.8/SCN10A antibody [EPR25132-222] (AB307817)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Nav1.8/SCN10A with ab307817 at 1/50 dilution (10.64 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green). Negative control : confocal image showing no staining on rat liver (PMID : 34409080). The section was incubated in two rounds of staining : in the order of ab307817 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nav1.8/SCN10A antibody [EPR25132-222] (AB307817)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat dorsal root ganglia (fresh) tissue labeling Nav1.8/SCN10A with ab307817 at 1/50 dilution (10.64 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green). Panel A : merged staining of anti-Nav1.8/SCN10A (ab307817, green) and anti-NeuN (ab190565, red) on rat dorsal root ganglia. Panel B : anti-Nav1.8/SCN10A stained on rat dorsal root ganglia. Panel C : anti-NeuN stained in neurons of rat dorsal root ganglia. The section was incubated in two rounds of staining : in the order of ab307817 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [EPR25132-222] (AB307817)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat spleenocytes labelling Nav1.8/SCN10A with ab307817 at 1/50 (10.64 μg/ml) (shown in Green).

Confocal image showing no staining in rat spleenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by secondary counterstain ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution.

Secondary antibody : ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was used at 1/1000 (2μg/ml)