Anti-Nav1.8/SCN10A (C-terminal) antibody [EPR30868-550]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Nav1.8/SCN10A (C-terminal) antibody [EPR30868-550] (AB326328)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse P0 DRG (fresh frozen) tissue labeling Nav1.8/SCN10A with ab326328 at 1/50 (9.84 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Confocal image showing positive staining on mouse P0 DRG.
Panel A : merged staining of anti-SCN10A (ab326328, green), anti-NeuN (ab190565, magenta) on mouse P0 DRG.
Panel B : anti-SCN10A stained on mouse P0 DRG.
Panel C : anti-NeuN stained in neurons of mouse P0 DRG.
The section was incubated in two rounds of staining : in the order of ab326328 and ab190565, for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A (C-terminal) antibody [EPR30868-550] (AB326328)

Immunohistochemical analysis of paraffin-embedded Mouse dorsal root ganglion (DRG) tissue labeling Nav1.8/SCN10A with ab326328 at 1/500 (0.984 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse DRG. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Nav1.8/SCN10A (C-terminal) antibody [EPR30868-550] (AB326328)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh frozen) tissue labeling Nav1.8/SCN10A with ab326328 at 1/50 (9.84 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Negative control : confocal image showing no staining on mouse spleen. The nuclear counterstain was DAPI (Blue). The section was incubated with ab326328 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A (C-terminal) antibody [EPR30868-550] (AB326328)

Immunohistochemical analysis of paraffin-embedded
(A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse SCN10A expression vector containing a his tag
(B) HEK-293T transfected with a human SCN10A expression vector containing a his tag
(C) HEK-293T transfected with a mouse SCN3A expression vector containing a his tag
(D) HEK-293T transfected with a mouse SCN4A expression vector containing a his tag
(E) HEK-293T transfected with a mouse SCN5A expression vector containing a his tag
(F) HEK-293T transfected with a mouse SCN9A expression vector containing a his tag
(G) HEK-293T transfected with a mouse SCN11A expression vector containing a his tag
(H) HEK-293T transfected with empty vector containing a his tag tissues labeling Nav1.8/SCN10A with ab326328 at 1/2000 (0.246 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a mouse SCN10A expression vector containing a his tag, (B) HEK-293T transfected with a human SCN10A expression vector containing a his tag, no staining on (C) HEK-293T transfected with a mouse SCN3A expression vector containing a his tag, (D) HEK-293T transfected with a mouse SCN4A expression vector containing a his tag, (E) HEK-293T transfected with a mouse SCN5A expression vector containing a his tag, (F) HEK-293T transfected with a mouse SCN9A expression vector containing a his tag, (G) HEK-293T transfected with a mouse SCN11A expression vector containing a his tag, (H) HEK-293T transfected with empty vector containing a his tag. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A (C-terminal) antibody [EPR30868-550] (AB326328)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Nav1.8/SCN10A with ab326328 at 1/500 (0.984 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse spleen. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins