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AB122370

Anti-NBAS antibody

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(2 Publications)

Rabbit Polyclonal NBAS antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NBAS aa 950-1050.

View Alternative Names

NAG, NBAS, NBAS subunit of NRZ tethering complex, Neuroblastoma-amplified gene protein, Neuroblastoma-amplified sequence

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NBAS antibody (AB122370)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NBAS antibody (AB122370)

Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab122370 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBAS antibody (AB122370)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBAS antibody (AB122370)

ab122370, at a 1/50 dilution, staining NBAS in paraffin embedded Human heart muscle tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NBAS aa 950-1050. The exact immunogen used to generate this antibody is proprietary information.

A2RRP1

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Proteins & Peptides

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Recombinant Human NBAS protein (GST tag N-Terminus)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NBAS protein also known as Neuroblastoma Amplified Sequence functions as an important player in intracellular trafficking and protein stability. It weighs approximately 237 kDa and is broadly expressed across various tissues with noticeable presence in the liver and brain. The NBAS protein forms part of the syntaxin-binding protein 5 (STXBP5) complex aligning itself with other cytoplasmic and membrane-associated proteins. Through its interaction with these cellular structures the NBAS protein facilitates proper protein transportation within the cells.
Biological function summary

The NBAS protein contributes to maintaining homeostasis by participating in endoplasmic reticulum-to-Golgi apparatus transport. This protein joins the syntaxin 18 complex an assembly of proteins that forms part of the broader secretion machinery in cells. Through this involvement NBAS ensures effective protein sorting and delivery which is critical for cell function and communication. Disruption in NBAS activity might lead to accumulation of unfolded proteins affecting overall cellular health.

Pathways

The NBAS protein plays a significant role in the vesicular transport and secretory pathways. It functions alongside proteins such as STX18 and presumed interactions with other members of the SNARE complex facilitating the fusion of vesicles with target membranes. By participating in these pathways NBAS ensures the smooth transition of proteins and contributes indirectly to processes like secretion and intracellular signaling.

NBAS mutations have been associated with Short Stature Onychodysplasia Facial Dysmorphism and Hypotrichosis (SOFT) syndrome as well as liver dysfunction. SOFT syndrome symptoms indicate the protein's involvement in bone growth and hair follicle development while liver dysfunction suggests its necessity in hepatic protein trafficking. In both cases the NBAS shares connections with proteins in similar pathways such as components of the syntaxin and SNARE family indicating a broader impact on cellular and systemic health when NBAS function is compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in Golgi-to-endoplasmic reticulum (ER) retrograde transport; the function is proposed to depend on its association in the NRZ complex which is believed to play a role in SNARE assembly at the ER (PubMed : 19369418). Required for normal embryonic development (By similarity). May play a role in the nonsense-mediated decay pathway of mRNAs containing premature stop codons (By similarity).
See full target information NBAS
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 133: PubMed37463447

2023

RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Launay,Montserrat Ruiz,Laura Planas-Serra,Edgard Verdura,Agustí Rodríguez-Palmero,Agatha Schlüter,Leire Goicoechea,Cristina Guilera,Josefina Casas,Felix Campelo,Emmanuelle Jouanguy,Jean-Laurent Casanova,Odile Boespflug-Tanguy,Maria Vazquez Cancela,Luis González Gutiérrez-Solana,Carlos Casasnovas,Estela Area-Gomez,Aurora Pujol

The Journal of cell biology 217:975-995 PubMed29367353

2018

Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Dijin Xu,Yuqi Li,Lizhen Wu,Ying Li,Dongyu Zhao,Jinhai Yu,Tuozhi Huang,Charles Ferguson,Robert G Parton,Hongyuan Yang,Peng Li
View all publications
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Product promise

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