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AB242220

Anti-NBAS antibody

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(1 Publication)

Rabbit Polyclonal NBAS antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NBAS aa 350-400.

View Alternative Names

NAG, NBAS, NBAS subunit of NRZ tethering complex, Neuroblastoma-amplified gene protein, Neuroblastoma-amplified sequence

2 Images
Immunoprecipitation - Anti-NBAS antibody (AB242220)
  • IP

Supplier Data

Immunoprecipitation - Anti-NBAS antibody (AB242220)

NBAS was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242220 at 3 μl/reaction.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-NBAS antibody (ab242220) at 1/1000 dilution

Lane 1:

ab242220 IP in Jurkat whole cell lysate

Lane 2:

Control IgG IP in Jurkat whole cell lysate

Predicted band size: 269 kDa

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Exposure time: 3min

Western blot - Anti-NBAS antibody (AB242220)
  • WB

Supplier Data

Western blot - Anti-NBAS antibody (AB242220)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-NBAS antibody (ab242220) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 269 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NBAS aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

A2RRP1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>3 - 6 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to NBAS immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NBAS protein also known as Neuroblastoma Amplified Sequence functions as an important player in intracellular trafficking and protein stability. It weighs approximately 237 kDa and is broadly expressed across various tissues with noticeable presence in the liver and brain. The NBAS protein forms part of the syntaxin-binding protein 5 (STXBP5) complex aligning itself with other cytoplasmic and membrane-associated proteins. Through its interaction with these cellular structures the NBAS protein facilitates proper protein transportation within the cells.
Biological function summary

The NBAS protein contributes to maintaining homeostasis by participating in endoplasmic reticulum-to-Golgi apparatus transport. This protein joins the syntaxin 18 complex an assembly of proteins that forms part of the broader secretion machinery in cells. Through this involvement NBAS ensures effective protein sorting and delivery which is critical for cell function and communication. Disruption in NBAS activity might lead to accumulation of unfolded proteins affecting overall cellular health.

Pathways

The NBAS protein plays a significant role in the vesicular transport and secretory pathways. It functions alongside proteins such as STX18 and presumed interactions with other members of the SNARE complex facilitating the fusion of vesicles with target membranes. By participating in these pathways NBAS ensures the smooth transition of proteins and contributes indirectly to processes like secretion and intracellular signaling.

NBAS mutations have been associated with Short Stature Onychodysplasia Facial Dysmorphism and Hypotrichosis (SOFT) syndrome as well as liver dysfunction. SOFT syndrome symptoms indicate the protein's involvement in bone growth and hair follicle development while liver dysfunction suggests its necessity in hepatic protein trafficking. In both cases the NBAS shares connections with proteins in similar pathways such as components of the syntaxin and SNARE family indicating a broader impact on cellular and systemic health when NBAS function is compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in Golgi-to-endoplasmic reticulum (ER) retrograde transport; the function is proposed to depend on its association in the NRZ complex which is believed to play a role in SNARE assembly at the ER (PubMed : 19369418). Required for normal embryonic development (By similarity). May play a role in the nonsense-mediated decay pathway of mRNAs containing premature stop codons (By similarity).
See full target information NBAS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:1211 PubMed38017066

2023

Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline E Strong,Jiajing Zhang,Martin Carrasco,Srikanya Kundu,Molly Boutin,Harshad D Vishwasrao,Jiamin Liu,Angelica Medina,Yu-Chi Chen,Kelli Wilson,Emily M Lee,Marc Ferrer
View all publications
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