AB174843

Anti-NBL1 antibody [EPR12397]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NBL1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DAN, DAND1, NBL1, Neuroblastoma suppressor of tumorigenicity 1, DAN domain family member 1, Protein N03, Zinc finger protein DAN

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NBL1 antibody [EPR12397] (AB174843)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NBL1 antibody [EPR12397] (AB174843)

Immunofluorescent analysis of A431 cells labeling NBL1 with ab174843 at 1/100 dilution (red). DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-NBL1 antibody [EPR12397] (AB174843)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NBL1 antibody [EPR12397] (AB174843)

Intracellular flow cytometric analysis of permeabilized A431 cells labeling NBL1 with ab174843 at 1/10 dilution (red), or negative control rabbit IgG (green).

Supplier Data

Western blot - Anti-NBL1 antibody [EPR12397] (AB174843)

All lanes:

Western blot - Anti-NBL1 antibody [EPR12397] (ab174843) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

Human prostate lysate at 10 µg

Predicted band size: 149 kDa,19 kDa

false

Carrier free

Anti-NBL1 antibody [EPR12397] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12397

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5001", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5001", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NBL1 also known as DAN domain family member 1 is a protein with a molecular weight of approximately 20 kDa. It is found in various tissues but shows higher expression levels in the nervous tissue and skeletal muscle. Mechanically NBL1 acts as a modulator in cell signaling pathways by binding to specific growth factors such as BMPs (Bone Morphogenetic Proteins) inhibiting their functions and affecting cell differentiation and growth.

Biological function summary

NBL1 plays an inhibitory role in bone morphogenesis by interacting with BMPs which are part of the TGF-beta superfamily. NBL1 can form complexes with these growth factors thereby regulating processes related to bone and cartilage development. This protein is essential for maintaining cellular processes involved in tissue homeostasis and suppressing unchecked cellular proliferation.

Pathways

NBL1 involves itself in the BMP signaling pathway where it directly impacts the regulation of bone and cartilage development. It is associated with other proteins like Noggin and Chordin both of which also modulate BMP activity highlighting its importance in balancing cell growth and differentiation signals. The involvement of NBL1 in the TGF-beta/BMP pathway is critical for embryonic development and the regulation of body patterning.

NBL1 has been linked to conditions such as osteoarthritis and certain cancer types. Its inhibitory effect on BMPs associates it with osteoarthritis where altered BMP signaling contributes to cartilage degradation. In cancer where BMP activity can be dysregulated NBL1's role in inhibiting BMP pathways places it in connection to tumor suppressors like SMAD proteins emphasizing its potential relevance in tumorigenesis and metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

See full target information NBL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer gene therapy 29:1878-1894 PubMed35840668

2022

MicroRNA-21 guide and passenger strand regulation of adenylosuccinate lyase-mediated purine metabolism promotes transition to an EGFR-TKI-tolerant persister state.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Cai Zhang,Nicholas Skiados,Fareesa Aftab,Cerena Moreno,Luis Silva,Paul Joshua Anthony Corbilla,John M Asara,Aaron N Hata,Frank J Slack

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NBL1 antibody [EPR12397]

Immunocytochemistry/ Immunofluorescence - Anti-NBL1 antibody [EPR12397] (AB174843)

Flow Cytometry (Intracellular) - Anti-NBL1 antibody [EPR12397] (AB174843)

Western blot - Anti-NBL1 antibody [EPR12397] (AB174843)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise