Mouse Monoclonal NBR1 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Recombinant fragment, Mouse, Human, Recombinant fragment - Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NBR1 aa 1-100.
Images
![Flow Cytometry - Anti-NBR1 antibody [5C3] (AB55474), expandable thumbnail](https://content.abcam.com/products/images/nbr1-antibody-5c3-ab55474--flow-cytometry-img38069.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-NBR1 antibody [5C3] (AB55474)")
![Western blot - Anti-NBR1 antibody [5C3] (AB55474), expandable thumbnail](https://content.abcam.com/products/images/nbr1-antibody-5c3-ab55474--western-blot-img147509.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NBR1 antibody [5C3] (AB55474)")
![Western blot - Anti-NBR1 antibody [5C3] (AB55474), expandable thumbnail](https://content.abcam.com/products/images/nbr1-antibody-5c3-ab55474--western-blot-img143144.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NBR1 antibody [5C3] (AB55474)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody [5C3] (AB55474), expandable thumbnail](https://content.abcam.com/products/images/nbr1-antibody-5c3-ab55474--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27754.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody [5C3] (AB55474)")
Publications
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- Cell host & microbe 30:1671-1684.e92022The bacterial effector GarD shields Chlamydia trachomatis inclusions from RNF213-mediated ubiquitylation and destruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen C Walsh,Jeffrey R Reitano,Mary S Dickinson,Miriam Kutsch,Dulcemaria Hernandez,Alyson B Barnes,Benjamin H Schott,Liuyang Wang,Dennis C Ko,So Young Kim,Raphael H Valdivia,Robert J Bastidas,Jörn CoersPubMed 36084633
- Scientific reports 12:116622022Lack of Cathepsin D in the central nervous system results in microglia and astrocyte activation and the accumulation of proteinopathy-related proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChigure Suzuki,Junji Yamaguchi,Takahito Sanada,Juan Alejandro Oliva Trejo,Souichirou Kakuta,Masahiro Shibata,Isei Tanida,Yasuo UchiyamaPubMed 35804072
- Autophagy 18:2926-29452022Chemical modulation of SQSTM1/p62-mediated xenophagy that targets a broad range of pathogenic bacteria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoon Jee Lee,Jin Kyung Kim,Chan Hoon Jung,Young Jae Kim,Eui Jung Jung,Su Hyun Lee,Ha Rim Choi,Yeon Sung Son,Sang Mi Shim,Sang Min Jeon,Jin Ho Choe,Sang-Hee Lee,Jake Whang,Kyung-Cheol Sohn,Gang Min Hur,Hyun Tae Kim,Jinki Yeom,Eun-Kyeong Jo,Yong Tae KwonPubMed 35316156
- Clinical and translational medicine 12:e7192022Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaxing Shen,Heng Zhang,Shihua Yao,Feng Su,Hao Wang,Jun Yin,Yong Fang,Lijie Tan,Kaiguang Zhang,Xiangshan Fan,Ming Zhong,Qingxin Zhou,Jie He,Zhiyong ZhangPubMed 35092699
- Nature communications 11:3072020Temporal inhibition of autophagy reveals segmental reversal of ageing with increased cancer risk.
- Autophagy 16:1092-11102019SQSTM1/p62 and PPARGC1A/PGC-1alpha at the interface of autophagy and vascular senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGloria Salazar,Abigail Cullen,Jingwen Huang,Yitong Zhao,Alexa Serino,Lula Hilenski,Nikolay Patrushev,Farshad Forouzandeh,Hyun Seok HwangPubMed 31441382
- Cell death & disease 9:7682018Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Lin et. al.PubMed 29991758
- Scientific reports 8:99722018Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhou Yu,Jing Ma,Xia Li,Yixiong Liu,Mingyang Li,Lu Wang,Ming Zhao,Huiying He,Yifen Zhang,Qiu Rao,Danhui Zhao,Yingmei Wang,Linni Fan,Peifeng Li,Yang Liu,Fang Liu,Feng Zhang,Jing Ye,Qingguo Yan,Shuangping Guo,Zhe WangPubMed 29967346
- Autophagy 14:764-7772018Atg9a deficiency causes axon-specific lesions including neuronal circuit dysgenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunji Yamaguchi et. al.PubMed 28513333
- Cell host & microbe 21:59-722016The Ubiquitin Ligase Smurf1 Functions in Selective Autophagy of Mycobacterium tuberculosis and Anti-tuberculous Host Defense.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuis H Franco,Vidhya R Nair,Caitlyn R Scharn,Ramnik J Xavier,Jose R Torrealba,Michael U Shiloh,Beth LevinePubMed 28017659
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human NBR1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q14596
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Tested |
| Mouse | Expected | Predicted | Predicted | Predicted |
| Recombinant fragment | Expected | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes Predicted molecular weight: 107 kDa. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed:24692539, PubMed:33577621). Possesses a double function during the selective autophagy by acting as a shuttle bringing ubiquitinated proteins to autophagosomes and also by participating in the formation of protein aggregates (PubMed:24879152, PubMed:34471133). Plays a role in the regulation of the innate immune response by modulating type I interferon production and targeting ubiquitinated IRF3 for autophagic degradation (PubMed:35914352). In response to oxidative stress, promotes an increase in SQSTM1 levels, phosphorylation, and body formation by preventing its autophagic degradation (By similarity). In turn, activates the KEAP1-NRF2/NFE2L2 antioxidant pathway (By similarity). Plays also non-autophagy role by mediating the shuttle of IL-12 to late endosome for subsequent secretion (By similarity).
Alternative names
1A13B, KIAA0049, M17S2, MIG19, NBR1, Next to BRCA1 gene 1 protein, Cell migration-inducing gene 19 protein, Membrane component chromosome 17 surface marker 2, Neighbor of BRCA1 gene 1 protein, Protein 1A1-3B
Recommended products
Mouse Monoclonal NBR1 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Recombinant fragment, Mouse, Human, Recombinant fragment - Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NBR1 aa 1-100.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human NBR1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q14596
- Carrier free
- Yes
- Clone number
- 5C3
- Purity
- Tissue culture supernatant
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 8/Oct/2019. Lot numbers higher than GR3274169 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NBR1 also known as Neighbor of BRCA1 Gene 1 functions as a receptor for selective autophagy. It recognizes ubiquitinated proteins and delivers them to autophagosomes for degradation. The protein has a molecular weight of approximately 110 kDa. Scientists have observed its expression in a variety of tissues with particularly high levels in the testis and low levels in the lung and kidney.
- Biological function summary
NBR1 plays a significant role in the removal of misfolded or damaged proteins through autophagy. It acts within the p62/SQSTM1 complex which links to the autophagic machinery. The complex recruits ubiquitinated substrates facilitating their degradation in lysosomes. Through this mechanism NBR1 helps maintain cellular homeostasis and genome integrity by removing potentially harmful elements.
- Pathways
NBR1 integrates into the critical cellular recycling process known as autophagy. It works closely with proteins such as p62/SQSTM1 in the ubiquitin-proteasome pathway to ensure efficient protein turnover. The protein participates in signals that control cell growth and survival influencing the mTOR pathway which is important in regulating cellular metabolism growth and life span.
- Associated diseases and disorders
Research indicates that NBR1 might have implications in neurodegenerative diseases like Alzheimer's and Parkinson's. Its involvement in autophagy suggests that malfunction or aberrant expression of NBR1 could lead to the accumulation of protein aggregates associated with these conditions. Additionally interactions with proteins such as LC3 and p62/SQSTM1 central in lysosomal degradation highlight its potential role in disease mechanisms where autophagy is disrupted.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-NBR1 antibody [5C3] (ab55474), expandable thumbnail](https://content.abcam.com/products/images/nbr1-antibody-5c3-ab55474--flow-cytometry-img38069.jpg "Flow Cytometry - Anti-NBR1 antibody [5C3] (ab55474)")
Flow Cytometry - Anti-NBR1 antibody [5C3] (ab55474)
Overlay histogram showing HeLa cells stained with ab55474 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55474, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
![Western blot - Anti-NBR1 antibody [5C3] (ab55474), expandable thumbnail](https://content.abcam.com/products/images/nbr1-antibody-5c3-ab55474--western-blot-img147509.jpg "Western blot - Anti-NBR1 antibody [5C3] (ab55474)")
Western blot - Anti-NBR1 antibody [5C3] (ab55474)
Western blot against tagged recombinant protein immunogen using ab55474 NBR1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-NBR1 antibody [5C3] (ab55474)
Predicted band size: 107 kDa
![Western blot - Anti-NBR1 antibody [5C3] (ab55474), expandable thumbnail](https://content.abcam.com/products/images/nbr1-antibody-5c3-ab55474--western-blot-img143144.jpg "Western blot - Anti-NBR1 antibody [5C3] (ab55474)")
Western blot - Anti-NBR1 antibody [5C3] (ab55474)
Western blot against tagged recombinant protein immunogen using ab55474 NBR1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-NBR1 antibody [5C3] (ab55474)
Predicted band size: 107 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody [5C3] (ab55474), expandable thumbnail](https://content.abcam.com/products/images/nbr1-antibody-5c3-ab55474--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27754.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody [5C3] (ab55474)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody [5C3] (ab55474)
ab55474 (4μg/ml) staining NBR1 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is moderate cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.This image was generated using the ascites version of the product.
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