AB126175

Anti-NBR1 antibody

Name: Anti-NBR1 antibody (ab126175) | Abcam
Brand: Abcam Limited
SKU: AB126175
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal NBR1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NBR1 aa 350-700.

View Alternative Names

1A13B, KIAA0049, M17S2, MIG19, NBR1, Next to BRCA1 gene 1 protein, Cell migration-inducing gene 19 protein, Membrane component chromosome 17 surface marker 2, Neighbor of BRCA1 gene 1 protein, Protein 1A1-3B

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody (AB126175)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody (AB126175)

ab126175, at 1/250 dilution, staining NBR1 in paraffin-embedded Human Breast cancer tissue by Immunohistochemistry.

Unknown

Western blot - Anti-NBR1 antibody (AB126175)

7.5% SDS PAGE

All lanes:

Western blot - Anti-NBR1 antibody (ab126175) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 107 kDa

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Supplier Data

Western blot - Anti-NBR1 antibody (AB126175)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-NBR1 antibody (ab126175) at 1/1000 dilution

Lane 1:

NIH-3T3 whole cell lysates at 30 µg

Lane 2:

JC whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 107 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NBR1 aa 350-700. The exact immunogen used to generate this antibody is proprietary information.

Q14596

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NBR1 also known as Neighbor of BRCA1 Gene 1 functions as a receptor for selective autophagy. It recognizes ubiquitinated proteins and delivers them to autophagosomes for degradation. The protein has a molecular weight of approximately 110 kDa. Scientists have observed its expression in a variety of tissues with particularly high levels in the testis and low levels in the lung and kidney.

Biological function summary

NBR1 plays a significant role in the removal of misfolded or damaged proteins through autophagy. It acts within the p62/SQSTM1 complex which links to the autophagic machinery. The complex recruits ubiquitinated substrates facilitating their degradation in lysosomes. Through this mechanism NBR1 helps maintain cellular homeostasis and genome integrity by removing potentially harmful elements.

Pathways

NBR1 integrates into the critical cellular recycling process known as autophagy. It works closely with proteins such as p62/SQSTM1 in the ubiquitin-proteasome pathway to ensure efficient protein turnover. The protein participates in signals that control cell growth and survival influencing the mTOR pathway which is important in regulating cellular metabolism growth and life span.

Research indicates that NBR1 might have implications in neurodegenerative diseases like Alzheimer's and Parkinson's. Its involvement in autophagy suggests that malfunction or aberrant expression of NBR1 could lead to the accumulation of protein aggregates associated with these conditions. Additionally interactions with proteins such as LC3 and p62/SQSTM1 central in lysosomal degradation highlight its potential role in disease mechanisms where autophagy is disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed : 24692539, PubMed : 33577621). Possesses a double function during the selective autophagy by acting as a shuttle bringing ubiquitinated proteins to autophagosomes and also by participating in the formation of protein aggregates (PubMed : 24879152, PubMed : 34471133, PubMed : 38169523). Plays a role in the regulation of the innate immune response by modulating type I interferon production and targeting ubiquitinated IRF3 for autophagic degradation (PubMed : 35914352). In response to oxidative stress, promotes an increase in SQSTM1 levels, phosphorylation, and body formation by preventing its autophagic degradation (By similarity). In turn, activates the KEAP1-NRF2/NFE2L2 antioxidant pathway (By similarity). Also plays non-autophagy role by mediating the shuttle of IL-12 to late endosome for subsequent secretion (By similarity).

See full target information NBR1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0325505 PubMed40465797

2025

Autophagy across tissues of aging mice.

Applications

Unspecified application

Species

Unspecified reactive species

Julian M Carosi,Alexis Martin,Leanne K Hein,Sofia Hassiotis,Kathryn J Hattersley,Bradley J Turner,Célia Fourrier,Julien Bensalem,Timothy J Sargeant

Circulation 146:1758-1778 PubMed36259389

2022

Mutant Phosphodiesterase 3A Protects From Hypertension-Induced Cardiac Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Ercu,Michael B Mücke,Tamara Pallien,Lajos Markó,Anastasiia Sholokh,Carolin Schächterle,Atakan Aydin,Alexa Kidd,Stephan Walter,Yasmin Esmati,Brandon J McMurray,Daniella F Lato,Daniele Yumi Sunaga-Franze,Philip H Dierks,Barbara Isabel Montesinos Flores,Ryan Walker-Gray,Maolian Gong,Claudia Merticariu,Kerstin Zühlke,Michael Russwurm,Tiannan Liu,Theda U P Batolomaeus,Sabine Pautz,Stefanie Schelenz,Martin Taube,Hanna Napieczynska,Arnd Heuser,Jenny Eichhorst,Martin Lehmann,Duncan C Miller,Sebastian Diecke,Fatimunnisa Qadri,Elena Popova,Reika Langanki,Matthew A Movsesian,Friedrich W Herberg,Sofia K Forslund,Dominik N Müller,Tatiana Borodina,Philipp G Maass,Sylvia Bähring,Norbert Hübner,Michael Bader,Enno Klussmann

Autophagy 17:3361-3374 PubMed33404288

2021

Tumor heterogeneity in autophagy-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingbo Li,Jiao Liu,Yinghua Xu,Runliu Wu,Xin Chen,Xinxin Song,Herbert Zeh,Rui Kang,Daniel J Klionsky,Xiaoyan Wang,Daolin Tang

Autophagy 17:3238-3255 PubMed33164641

2020

Measurement of autophagic flux in humans: an optimized method for blood samples.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Bensalem,Kathryn J Hattersley,Leanne K Hein,Xiao Tong Teong,Julian M Carosi,Sofia Hassiotis,Randall H Grose,Célia Fourrier,Leonie K Heilbronn,Timothy J Sargeant

The Journal of biological chemistry 295:13584-13600 PubMed32732290

2020

The insufficiency of ATG4A in macroautophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan Nguyen,Taryn J Olivas,Antonio Mires,Jiaxin Jin,Shenliang Yu,Lin Luan,Shanta Nag,Karlina J Kauffman,Thomas J Melia

Autophagy :1-15 PubMed31964216

2020

Loss of HSPA9 induces peroxisomal degradation by increasing pexophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Doo Sin Jo,So Jung Park,Ae-Kyeong Kim,Na Yeon Park,Joon Bum Kim,Ji-Eun Bae,Hyun Jun Park,Ji Hyun Shin,Jong Wook Chang,Peter K Kim,Yong-Keun Jung,Jae-Young Koh,Seong-Kyu Choe,Kyu-Sun Lee,Dong-Hyung Cho

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NBR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody (AB126175)

Western blot - Anti-NBR1 antibody (AB126175)

Western blot - Anti-NBR1 antibody (AB126175)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise