AB226360

Anti-NBR1 antibody - C-terminal

Name: Anti-NBR1 antibody - C-terminal (ab226360) | Abcam
Brand: Abcam Limited
SKU: AB226360
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal NBR1 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NBR1 aa 900 to C-terminus.

View Alternative Names

1A13B, KIAA0049, M17S2, MIG19, NBR1, Next to BRCA1 gene 1 protein, Cell migration-inducing gene 19 protein, Membrane component chromosome 17 surface marker 2, Neighbor of BRCA1 gene 1 protein, Protein 1A1-3B

2 Images

Supplier Data

Immunoprecipitation - Anti-NBR1 antibody - C-terminal (AB226360)

NBR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226360 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226360 at 1 μg/ml.

Lane 1 : ab226360 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-NBR1 antibody - C-terminal (ab226360)

Predicted band size: 107 kDa

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Supplier Data

Western blot - Anti-NBR1 antibody - C-terminal (AB226360)

Cell lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-NBR1 antibody - C-terminal (ab226360) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 107 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NBR1 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14596

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226360 was affinity purified using an epitope specific to NBR1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NBR1 also known as Neighbor of BRCA1 Gene 1 functions as a receptor for selective autophagy. It recognizes ubiquitinated proteins and delivers them to autophagosomes for degradation. The protein has a molecular weight of approximately 110 kDa. Scientists have observed its expression in a variety of tissues with particularly high levels in the testis and low levels in the lung and kidney.

Biological function summary

NBR1 plays a significant role in the removal of misfolded or damaged proteins through autophagy. It acts within the p62/SQSTM1 complex which links to the autophagic machinery. The complex recruits ubiquitinated substrates facilitating their degradation in lysosomes. Through this mechanism NBR1 helps maintain cellular homeostasis and genome integrity by removing potentially harmful elements.

Pathways

NBR1 integrates into the critical cellular recycling process known as autophagy. It works closely with proteins such as p62/SQSTM1 in the ubiquitin-proteasome pathway to ensure efficient protein turnover. The protein participates in signals that control cell growth and survival influencing the mTOR pathway which is important in regulating cellular metabolism growth and life span.

Research indicates that NBR1 might have implications in neurodegenerative diseases like Alzheimer's and Parkinson's. Its involvement in autophagy suggests that malfunction or aberrant expression of NBR1 could lead to the accumulation of protein aggregates associated with these conditions. Additionally interactions with proteins such as LC3 and p62/SQSTM1 central in lysosomal degradation highlight its potential role in disease mechanisms where autophagy is disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed : 24692539, PubMed : 33577621). Possesses a double function during the selective autophagy by acting as a shuttle bringing ubiquitinated proteins to autophagosomes and also by participating in the formation of protein aggregates (PubMed : 24879152, PubMed : 34471133). Plays a role in the regulation of the innate immune response by modulating type I interferon production and targeting ubiquitinated IRF3 for autophagic degradation (PubMed : 35914352). In response to oxidative stress, promotes an increase in SQSTM1 levels, phosphorylation, and body formation by preventing its autophagic degradation (By similarity). In turn, activates the KEAP1-NRF2/NFE2L2 antioxidant pathway (By similarity). Plays also non-autophagy role by mediating the shuttle of IL-12 to late endosome for subsequent secretion (By similarity).

See full target information NBR1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:1172 PubMed33608523

2021

20S proteasomes secreted by the malaria parasite promote its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elya Dekel,Dana Yaffe,Irit Rosenhek-Goldian,Gili Ben-Nissan,Yifat Ofir-Birin,Mattia I Morandi,Tamar Ziv,Xavier Sisquella,Matthew A Pimentel,Thomas Nebl,Eugene Kapp,Yael Ohana Daniel,Paula Abou Karam,Daniel Alfandari,Ron Rotkopf,Shimrit Malihi,Tal Block Temin,Debakshi Mullick,Or-Yam Revach,Ariel Rudik,Nir S Gov,Ido Azuri,Ziv Porat,Giulia Bergamaschi,Raya Sorkin,Gijs J L Wuite,Ori Avinoam,Teresa G Carvalho,Sidney R Cohen,Michal Sharon,Neta Regev-Rudzki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NBR1 antibody - C-terminal

Immunoprecipitation - Anti-NBR1 antibody - C-terminal (AB226360)

Western blot - Anti-NBR1 antibody - C-terminal (AB226360)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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