AB9272

Anti-NCAM1 antibody [123C3]

Name: Anti-NCAM1 antibody [123C3] (ab9272) | Abcam
Brand: Abcam Limited
SKU: AB9272
Price: 520 USD
Availability: InStock
Rating: 2 (4 reviews)

(4 Reviews)

(28 Publications)

Mouse Monoclonal NCAM1 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Human, Zebrafish samples. Cited in 28 publications.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [123C3] (AB9272)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [123C3] (AB9272)

Immunostaining of the neural cell adhesion molecule (NCAM) in small cell human lung cancer cell line NCI-H82.

Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (AB9272)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (AB9272)

Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone : Methanol 1 : 1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (AB9272)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (AB9272)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

123C3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-Fr, ICC/IF

applications

Epitope

This antibody recognizes an epitope in the NCAM exons 11-13 which is dependent on an intact conformation of the first fibronectin type-III homologous domain encoded by these exons.

Specificity

This antibody stains neural cell adhesion molecule (NCAM) which is present in small cell lung cancer and lung carcinoids. It also reacts with a number of non-small cell lung carcinomas and neuroendocrine and neuronal derived tissues. In addition, it is internalized after binding to its antigen on SCLC cell lines, making it an excellent reagent for tumor imaging in xenograft models. ab9272 reacts with all three isoforms of NCAM.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500 - 1/1000", "IHCFr-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.

See full target information NCAM1

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:88 PubMed39677412

2024

The role of IL‑17, IFN‑γ, 4‑1BBL and tumour‑infiltrating lymphocytes in the occurrence, development and prognosis of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Liu,Ke Zhang,Xiaodi Cai,Jikai Zhou,Yixuan Cai,Yujie Gu,Tingting Xia,Jianxin Ye

JCI insight 9: PubMed39405112

2024

Type 2 innate immunity promotes the development of pulmonary fibrosis in Hermansky-Pudlak syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Parand Sorkhdini,Kiran Klubock-Shukla,Selena Sheth,Dongqin Yang,Alina Xiaoyu Yang,Carmelissa Norbrun,Wendy J Introne,Bernadette R Gochuico,Yang Zhou

Regenerative biomaterials 11:rbad116 PubMed38333727

2024

Harnessing cytokine-induced killer cells to accelerate diabetic wound healing: an approach to regulating post-traumatic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yixi Yang,Cheng Zhang,Yuan Jiang,Yijun He,Jiawei Cai,Lin Liang,Zhaohuan Chen,Sicheng Pan,Chu Hua,Keke Wu,Le Wang,Zhiyong Zhang

Cell biology international 46:2185-2197 PubMed36116109

2022

Bone marrow mesenchymal stem cells-derived exosomes mediate nuclear receptor coactivator-3 expression in osteoblasts by delivering miR-532-5p to influence osteonecrosis of the femoral head development.

Applications

Unspecified application

Species

Unspecified reactive species

XiaoYong Lan,HaiPing Ma,YiPin Xiong,LingFeng Zou,Zhen Yuan,YuHong Xiao

Cells 11: PubMed35011706

2022

New Therapy for Spinal Cord Injury: Autologous Genetically-Enriched Leucoconcentrate Integrated with Epidural Electrical Stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Rustem Islamov,Farid Bashirov,Andrei Izmailov,Filip Fadeev,Vage Markosyan,Mikhail Sokolov,Maksim Shmarov,Denis Logunov,Boris Naroditsky,Igor Lavrov

Aging 13:19750-19759 PubMed34351305

2021

Dual inhibition of FGFR4 and BCL-xL inhibits multi-resistant ovarian cancer with BCL2L1 gain.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Guo,Chao Gu,Bin Li,Congjian Xu

Oncoimmunology 10:1907059 PubMed33889438

2021

VISTA and PD-L1 synergistically predict poor prognosis in patients with extranodal natural killer/T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Xia He,Yan Gao,Jian-Chang Fu,Qiang-Hua Zhou,Xiao-Xiao Wang,Bing Bai,Peng-Fei Li,Cheng Huang,Qi-Xiang Rong,Li-Qin Ping,Yan-Xia He,Jia-Ying Mao,Xu Chen,Hui-Qiang Huang

Seminars in thoracic and cardiovascular surgery 34:349-358 PubMed33711463

2021

Autologous Multilayered Fibroblast Sheets Can Reinforce Bronchial Stump in a Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sota Yoshimine,Koji Ueno,Junichi Murakami,Toshiro Saito,Ryo Suzuki,Yoshiyuki Asai,Eiji Ikeda,Toshiki Tanaka,Kimikazu Hamano

International journal of molecular medicine 47: PubMed33576467

2021

Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Tong,Jia Li,Xinzhe Feng,Chen Wang,Yihong Xu,Chongru He,Weidong Xu

Oxidative medicine and cellular longevity 2020:6372935 PubMed32089774

2020

AVE0991, a Nonpeptide Angiotensin 1-7 Receptor Agonist, Improves Glucose Metabolism in the Skeletal Muscle of Obese Zucker Rats: Possible Involvement of Prooxidant/Antioxidant Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Viktoria Dobrocsyova,Miroslava Slamkova,Katarina Krskova,Lucia Balazova,Maciej Suski,Rafal Olszanecki,Sona Cacanyiova,Stefan Zorad

View all publications

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Assay Kits

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