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Mouse Monoclonal NCAM1 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Human, Zebrafish samples. Cited in 25 publications.


Images

Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (AB9272), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (AB9272), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (AB9272), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [123C3] (AB9272), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-Fr
Human
Tested
Expected
Zebrafish
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Zebrafish
Dilution info
1/500.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.

Alternative names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

Recommended products

Mouse Monoclonal NCAM1 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Human, Zebrafish samples. Cited in 25 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Clone number
123C3
Purification technique
Affinity purification Protein G
Specificity

This antibody stains neural cell adhesion molecule (NCAM) which is present in small cell lung cancer and lung carcinoids. It also reacts with a number of non-small cell lung carcinomas and neuroendocrine and neuronal derived tissues. In addition, it is internalized after binding to its antigen on SCLC cell lines, making it an excellent reagent for tumor imaging in xenograft models. ab9272 reacts with all three isoforms of NCAM.

Epitope
This antibody recognizes an epitope in the NCAM exons 11-13 which is dependent on an intact conformation of the first fibronectin type-III homologous domain encoded by these exons.
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

Associated diseases and disorders

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)

    Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

  • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)

    Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

  • Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-NCAM1 antibody [123C3] (ab9272)

    Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [123C3] (ab9272), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [123C3] (ab9272)

    Immunostaining of the neural cell adhesion molecule (NCAM) in small cell human lung cancer cell line NCI-H82.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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