Skip to main content

Mouse Monoclonal NCAM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, African green monkey, Dog, Human, Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCAM1 aa 1-750.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [1G4] (AB230724), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [1G4] (AB230724), expandable thumbnail
  • Western blot - Anti-NCAM1 antibody [1G4] (AB230724), expandable thumbnail
  • Western blot - Anti-NCAM1 antibody [1G4] (AB230724), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human NCAM1 aa 1-750. The exact immunogen used to generate this antibody is proprietary information. Database link P13591

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
African green monkey
Tested
Expected
Cow
Predicted
Predicted
Dog
Tested
Expected
Transfected cell lysate
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
Rat
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
African green monkey
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
Dog
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
Human
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Species
Transfected cell lysate
Dilution info
1/200.00000 - 1/4000.00000
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Species
Dog
Dilution info
-
Notes

-

Species
African green monkey
Dilution info
10 µg/mL
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.

Alternative names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

Recommended products

Mouse Monoclonal NCAM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, African green monkey, Dog, Human, Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCAM1 aa 1-750.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human NCAM1 aa 1-750. The exact immunogen used to generate this antibody is proprietary information. Database link P13591
Clone number
1G4
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

Associated diseases and disorders

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [1G4] (ab230724), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [1G4] (ab230724)

    Formalin-fixed, paraffin-embedded human cerebellum tissue stained for NCAM1 using ab230724 at 10 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [1G4] (ab230724), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [1G4] (ab230724)

    Formalin-fixed, paraffin-embedded human cortex tissue stained for NCAM1 using ab230724 at 10 μg/ml in immunohistochemical analysis.

  • Western blot - Anti-NCAM1 antibody [1G4] (ab230724), expandable thumbnail

    Western blot - Anti-NCAM1 antibody [1G4] (ab230724)

    All lanes: Western blot - Anti-NCAM1 antibody [1G4] (ab230724) at 1/200 dilution

    Lane 1: pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

    Lane 2: pCMV6-ENTRY NCAM1-transfected HEK-293T whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 94 kDa

  • Western blot - Anti-NCAM1 antibody [1G4] (ab230724), expandable thumbnail

    Western blot - Anti-NCAM1 antibody [1G4] (ab230724)

    All lanes: Western blot - Anti-NCAM1 antibody [1G4] (ab230724) at 1/200 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

    Lane 3: SVT2 whole cell lysate at 35 µg

    Lane 4: A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

    Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

    Lane 7: MDCK (canine kidney cell line) whole cell lysate at 35 µg

    Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

    Lane 9: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 94 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com