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AB118291

Anti-NCAM1 antibody

3

(2 Reviews)

|

(2 Publications)

Goat Polyclonal NCAM1 antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NCAM1 aa 350-400.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

2 Images
Flow Cytometry - Anti-NCAM1 antibody (AB118291)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NCAM1 antibody (AB118291)

Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor® 488 secondary antibody (1 μg/ml).

IgG control : Non-immunized goat IgG (black line) followed by Alexa Fluor 488® secondary antibody.

Western blot - Anti-NCAM1 antibody (AB118291)
  • WB

Unknown

Western blot - Anti-NCAM1 antibody (AB118291)

The observed molecular weight corresponds to the glycosylated form of the protein.

All lanes:

Western blot - Anti-NCAM1 antibody (ab118291) at 0.01 µg/mL

All lanes:

Human frontal cortex lysate (in RIPA buffer) at 35 µg

Predicted band size: 94 kDa

Observed band size: 170 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human NCAM1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P13591

Specificity

ab118291 is expected to recognize reported isoform 2 (NP_851996.2) only.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to the glycosylated form.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab118291 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer communications (London, England) 45:172-177 PubMed39654335

2024

The interplay between natural killer cells and pancreatic stellate cells in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Elizabeth Ann Fincham,Parthiban Periasamy,Craig Ryan Joseph,Jia Meng,Jeffrey Chun Tatt Lim,Felicia Wee,Konstantinos Stasinos,Michelle Rodrigues Goulart,Jiangfeng Ye,Li Yen Chong,Bijin Veonice Au,Denise Goh,Joe Poh Sheng Yeong,Hemant Mahendrakumar Kocher

Molecular cancer research : MCR 21:411-427 PubMed36669126

2023

BAP1 Loss Is Associated with Higher ASS1 Expression in Epithelioid Mesothelioma: Implications for Therapeutic Stratification.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Barnett,Jenna Kenyani,Martina Tripari,Zohra Butt,Rudi Grosman,Francesca Querques,Liam Shaw,Luisa C Silva,Zoe Goate,Stefan J Marciniak,Doris M Rassl,Richard Jackson,Lu-Yun Lian,Peter W Szlosarek,Joseph J Sacco,Judy M Coulson
View all publications
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Product promise

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