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AB154566

Anti-NCAM1 antibody

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(1 Publication)

Rabbit Polyclonal NCAM1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCAM1 aa 1-400.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody (AB154566)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody (AB154566)

Immunofluorescence analysis of NK-92 cells fixed in 4% paraformaldehyde at RT for 15 min labelling NCAM protein using ab154566 at a 1/100 dilution (Green). DAPI was used for nuclear staining (Blue).

Western blot - Anti-NCAM1 antibody (AB154566)
  • WB

Supplier Data

Western blot - Anti-NCAM1 antibody (AB154566)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-NCAM1 antibody (ab154566) at 1/10000 dilution

All lanes:

Human brain tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 95 kDa

false

Western blot - Anti-NCAM1 antibody (AB154566)
  • WB

Supplier Data

Western blot - Anti-NCAM1 antibody (AB154566)

Samples were separated by 5% SDS PAGE.

All lanes:

Western blot - Anti-NCAM1 antibody (ab154566) at 1/1000 dilution

All lanes:

NK-92 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 95 kDa

false

Western blot - Anti-NCAM1 antibody (AB154566)
  • WB

Supplier Data

Western blot - Anti-NCAM1 antibody (AB154566)

Samples were separated by 5% SDS PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-NCAM1 antibody (ab154566) at 1/10000 dilution

Lane 1:

Mouse brain tissue lysate at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 95 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NCAM1 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P13591

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:9950 PubMed31289315

2019

Acute stress-induced change in polysialic acid levels mediated by sialidase in mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Chikara Abe,Yang Yi,Masaya Hane,Ken Kitajima,Chihiro Sato
View all publications
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Product promise

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