AB204446

Anti-NCAM1 antibody

Name: Anti-NCAM1 antibody (ab204446) | Abcam
Brand: Abcam Limited
SKU: AB204446
Price: 510 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal NCAM1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCAM1 aa 450-600.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody (AB204446)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody (AB204446)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling NCAM with ab204446 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody (AB204446)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody (AB204446)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling NCAM with ab204446 at 4μg/ml (green).

Supplier Data

Western blot - Anti-NCAM1 antibody (AB204446)

All lanes:

Western blot - Anti-NCAM1 antibody (ab204446)

All lanes:

Human cerebral cortex

Secondary

All lanes:

NCAM

Predicted band size: 20 kDa,50 kDa,61 kDa,94 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NCAM1 aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

P13591

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.

See full target information NCAM1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 19:1781-1802 PubMed36541703

2022

PEX13 prevents pexophagy by regulating ubiquitinated PEX5 and peroxisomal ROS.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas D Demers,Victoria Riccio,Doo Sin Jo,Sushil Bhandari,Kelsey B Law,Weifang Liao,Choy Kim,G Angus McQuibban,Seong-Kyu Choe,Dong-Hyung Cho,Peter K Kim

Experimental and therapeutic medicine 22:828 PubMed34149874

2021

MEF/KSF-conditioned culture medium: An effective method for culture of mouse dermal papilla cells with osteogenic differentiation potential.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Xu,Wenlan Gao,Shanshan Bai,Huichuan Duan,Xiaogang Pan,Wei Wu

Nature cell biology 23:652-663 PubMed34083785

2021

Non-invasive and high-throughput interrogation of exon-specific isoform expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Jiunn Jeffery Truong,Teeradon Phlairaharn,Bianca Eßwein,Christoph Gruber,Deniz Tümen,Enikő Baligács,Niklas Armbrust,Francesco Leandro Vaccaro,Eva-Maria Lederer,Eva Magdalena Beck,Julian Geilenkeuser,Simone Göppert,Luisa Krumwiede,Christian Grätz,Gerald Raffl,Dominic Schwarz,Martin Zirngibl,Milica Živanić,Maren Beyer,Johann Dietmar Körner,Tobias Santl,Valentin Evsyukov,Tabea Strauß,Sigrid C Schwarz,Günter U Höglinger,Peter Heutink,Sebastian Doll,Marcus Conrad,Florian Giesert,Wolfgang Wurst,Gil Gregor Westmeyer

International journal of molecular sciences 22: PubMed33669272

2021

Guide Cells Support Muscle Regeneration and Affect Neuro-Muscular Junction Organization.

Applications

Unspecified application

Species

Unspecified reactive species

Flavio L Ronzoni,Nefele Giarratana,Stefania Crippa,Mattia Quattrocelli,Marco Cassano,Gabriele Ceccarelli,Laura Benedetti,Jens Van Herck,Maria G Cusella De Angelis,Marco Vitale,Daniela Galli,Maurilio Sampaolesi

Journal of molecular neuroscience : MN 71:1174-1184 PubMed33175349

2020

LncRNA Neat1 Promotes Regeneration after Spinal Cord Injury by Targeting miR-29b.

Applications

Unspecified application

Species

Unspecified reactive species

Guangtao Bai,Liang Jiang,Pingping Meng,Jiang Li,Chao Han,Yuyang Wang,Qiang Wang

Neural regeneration research 16:550-560 PubMed32985487

2020

Combination of epidural electrical stimulation with triple gene therapy for spinal cord injury: a proof of principle study.

Applications

Unspecified application

Species

Unspecified reactive species

Filip Olegovich Fadeev,Farid Vagizovich Bashirov,Vahe Arshaluysovich Markosyan,Andrey Alexandrovich Izmailov,Tatyana Vyacheslavovna Povysheva,Mikhail Evgenyevich Sokolov,Maxim Sergeevich Kuznetsov,Anton Alexandrovich Eremeev,Ilnur Ildusovich Salafutdinov,Albert Anatolyevich Rizvanov,Hyun Joon Lee,Rustem Robertovich Islamov

View all publications

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