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AB237708

Anti-NCAM1 antibody [CAL53]

5

(2 Reviews)

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(4 Publications)

Anti-NCAM1 antibody [CAL53] (ab237708) is a rabbit monoclonal antibody detecting NCAM1 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [CAL53] (AB237708)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [CAL53] (AB237708)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NCAM1 with ab237708 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the human cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunoprecipitation - Anti-NCAM1 antibody [CAL53] (AB237708)
  • IP

Lab

Immunoprecipitation - Anti-NCAM1 antibody [CAL53] (AB237708)

NCAM1 was immunoprecipitated from 0.35 mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab237708 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab237708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : SH-SY5Y whole cell lysate 10 μg (Input).
Lane 2 : ab237708 IP in SH-SY5Y whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab237708 in SH-SY5Y whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-NCAM1 antibody [CAL53] (ab237708)

Predicted band size: 94 kDa

Observed band size: 140-180 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [CAL53] (AB237708)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [CAL53] (AB237708)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling NCAM1 with ab237708 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing membranous staining in Neuro-2a cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

PCS only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control : L-929 (PMID : 9696812).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [CAL53] (AB237708)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [CAL53] (AB237708)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NCAM1 with ab237708 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on islet in the rat cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [CAL53] (AB237708)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [CAL53] (AB237708)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NCAM1 with ab237708 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on islet in the mouse cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Western blot - Anti-NCAM1 antibody [CAL53] (AB237708)
  • WB

Lab

Western blot - Anti-NCAM1 antibody [CAL53] (AB237708)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NCAM1 antibody [CAL53] (ab237708) at 1/1000 dilution

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Human cerebellum lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma cell line0 whole cell lysate at 20 µg

Lane 4:

Mouse cerebellum lysate at 20 µg

Lane 5:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 140-180 kDa

false

Exposure time: 26s

  • Carrier free

    Anti-NCAM1 antibody [CAL53] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

CAL53

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-NCAM1 antibody [CAL53] (ab237708) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of NCAM1?
Anti-NCAM1 [CAL53] (ab237708) specifically detects a band for NCAM1 (UniProt: P13591) at a molecular weight of 140kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10μl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [CAL53] also available for your convenience: ab237708, Carrier free - ab251595

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purity >99%
Storage buffer
pH: 7.5 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:13432 PubMed38862586

2024

Combined oral low-dose cyclophosphamide endocrine therapy may improve clinical response among patients with metastatic breast cancer via Tregs in TLSs.

Applications

Unspecified application

Species

Unspecified reactive species

Yuze Zhao,Shuo Wang,Shuzhen Lv,Xiaojun Liu,Weiping Li,Yuguang Song,Dongwen Rong,Peiming Zheng,Hongyan Huang,Huixia Zheng

Signal transduction and targeted therapy 8:327 PubMed37661226

2023

Generation of inactivated IL2RG and RAG1 monkeys with severe combined immunodeficiency using base editing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zheng,Chunhui Huang,Yingqi Lin,Bofeng Han,Yizhi Chen,Caijuan Li,Jiawei Li,Yongyan Ding,Xichen Song,Wei Wang,Weien Liang,Jianhao Wu,Jiaxi Wu,Jiale Gao,Chengxi Wei,Xudong Zhang,Zhuchi Tu,Sen Yan

Cancer science 114:2650-2663 PubMed36846943

2023

ELF1 suppresses autophagy to reduce cisplatin resistance via the miR-152-3p/NCAM1/ERK axis in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lifeng Zhao,Xiangsheng Wu,Zhiwen Zhang,Lini Fang,Bo Yang,Yepeng Li

Cell reports. Medicine 3:100597 PubMed35492247

2022

Autoantibodies against NCAM1 from patients with schizophrenia cause schizophrenia-related behavior and changes in synapses in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Shiwaku,Shingo Katayama,Kanoh Kondo,Yuri Nakano,Hikari Tanaka,Yuki Yoshioka,Kyota Fujita,Haruna Tamaki,Hironao Takebayashi,Omi Terasaki,Yukihiro Nagase,Teruyoshi Nagase,Tetsuo Kubota,Kinya Ishikawa,Hitoshi Okazawa,Hidehiko Takahashi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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