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Anti-NCAM1 antibody [EP2567Y] (ab75813) is a rabbit monoclonal antibody detecting NCAM1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

- Over 50 publications



Images

Western blot - Anti-NCAM1 antibody [EP2567Y] (AB75813), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] (AB75813), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] (AB75813), expandable thumbnail
  • Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] (AB75813), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] (AB75813), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/10000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/20
Notes

For unpurified use at 1:100 dilution

Tested
Tested

Species
Human
Dilution info
1/50000 - 1/100000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified use at 1:100 - 1:250 dilution range

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1:1000 dilution.

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.

Alternative names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

Recommended products

Anti-NCAM1 antibody [EP2567Y] (ab75813) is a rabbit monoclonal antibody detecting NCAM1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

- Over 50 publications


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP2567Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-NCAM1 antibody [EP2567Y] (ab75813) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of NCAM1?


Anti-NCAM1 [EP2567Y] (ab75813) specifically detects a band for NCAM1 (UniProt: P13591) at a molecular weight of 93kDa.

Trusted by the scientific community


Anti-NCAM1 [EP2567Y] (ab75813) was first used in a scientific publication in 2012 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists


Anti-NCAM1 [EP2567Y] (ab75813) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EP2567Y] also available for your convenience:
ab75813, Carrier free - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free ab214435, Carrier free - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free ab215981, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-NCAM1 antibody [EP2567Y] ab237455, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y] ab237456, PE - PE Anti-NCAM1 antibody [EP2567Y] ab237457, APC - APC Anti-NCAM1 antibody [EP2567Y] ab303037, HRP - HRP Anti-NCAM1 antibody [EP2567Y] ab303038, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-NCAM1 antibody [EP2567Y] ab303718, Alkaline Phosphatase - Alkaline Phosphatase Anti-NCAM1 antibody [EP2567Y] ab308689, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-NCAM1 antibody [EP2567Y] ab311923, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-NCAM1 antibody [EP2567Y] ab312394, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-NCAM1 antibody [EP2567Y] ab321697



Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

Associated diseases and disorders

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-NCAM1 antibody [EP2567Y] (ab75813), expandable thumbnail

    Western blot - Anti-NCAM1 antibody [EP2567Y] (ab75813)

    All lanes: Western blot - Anti-NCAM1 antibody [EP2567Y] (ab75813) at 1/10000 dilution

    All lanes: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] (ab75813), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] (ab75813)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling NCAM1 with Purified ab75813 at 1/10000 dilution (0.02 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. PBS instead of the primary antibody was used as the negative control.

  • Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] (ab75813), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] (ab75813)

    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling NCAM1 with Purified ab75813 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] (ab75813), expandable thumbnail

    Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] (ab75813)

    NCAM1 was immunoprecipitated from 0.35 mg SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 μg with ab75813 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 μg

    Lane 2: ab75813 IP in SH-SY5Y whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab75813 in SH-SY5Y whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] (ab75813)

    Predicted band size: 94 kDa

    Observed band size: 180 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] (ab75813), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] (ab75813)

    Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling NCAM1 with Purified ab75813 at 1:50 dilution (3.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] (ab75813), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] (ab75813)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling NCAM1 with Purified ab75813 at 1:10000 dilution (0.0156 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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