Anti-NCAM1 [EP2567Y] antibody (ab214435) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect NCAM1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Images
![Western blot - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--western-blot-img173563.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166760.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)")
![Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--flow-cytometry-intracellular-img179063.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)")
![Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--immunoprecipitation-img176915.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)")
![Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--immunocytochemistry-immunofluorescence-img172854.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)")
Publications
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- Nature cancer 2:545-5622021Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheri A C McDowell,Robin B E Luo,Azadeh Arabzadeh,Samuel Doré,Nicolas C Bennett,Valérie Breton,Elham Karimi,Morteza Rezanejad,Ryan R Yang,Katherine D Lach,Marianne S M Issac,Bozena Samborska,Lucas J M Perus,Dan Moldoveanu,Yuhong Wei,Benoit Fiset,Roni F Rayes,Ian R Watson,Lawrence Kazak,Marie-Christine Guiot,Pierre O Fiset,Jonathan D Spicer,Andrew J Dannenberg,Logan A Walsh,Daniela F QuailPubMed 35122017
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Version
4 products for Alternative Product
Target data
Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.
Alternative names
CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1
Recommended products
Anti-NCAM1 [EP2567Y] antibody (ab214435) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect NCAM1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP2567Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of NCAM1?
Anti-NCAM1 [EP2567Y] - BSA and Azide free (ab214435) specifically detects a band for NCAM1 (UniProt: P13591) at a molecular weight of 94kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EP2567Y] also available for your convenience:
Anti-NCAM1 antibody [EP2567Y] ab75813, Carrier free - ab214435, Carrier free - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free ab215981, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-NCAM1 antibody [EP2567Y] ab237455, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y] ab237456, PE - PE Anti-NCAM1 antibody [EP2567Y] ab237457, APC - APC Anti-NCAM1 antibody [EP2567Y] ab303037, HRP - HRP Anti-NCAM1 antibody [EP2567Y] ab303038, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-NCAM1 antibody [EP2567Y] ab303718, Alkaline Phosphatase - Alkaline Phosphatase Anti-NCAM1 antibody [EP2567Y] ab308689, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-NCAM1 antibody [EP2567Y] ab311923, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-NCAM1 antibody [EP2567Y] ab312394, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-NCAM1 antibody [EP2567Y] ab321697
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
- Biological function summary
NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.
- Pathways
The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.
- Associated diseases and disorders
NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--western-blot-img173563.jpg "Western blot - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)")
Western blot - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)
All lanes: Western blot - Anti-NCAM1 antibody [EP2567Y] (Anti-NCAM1 antibody [EP2567Y] ab75813) at 1/10000 dilution
All lanes: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 94 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166760.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)
This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling NCAM1 with Purified Anti-NCAM1 antibody [EP2567Y] ab75813 at 1:10000 dilution (0.0156 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.![Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--flow-cytometry-intracellular-img179063.jpg "Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)")
Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)
This data was developed using ab214435, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling NCAM1 with Purified ab214435 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).![Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--immunoprecipitation-img176915.jpg "Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)")
Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)
This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.NCAM1 was immunoprecipitated from 0.35 mg SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg with Anti-NCAM1 antibody [EP2567Y] ab75813 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg
Lane 2: abab75813 IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NCAM1 antibody [EP2567Y] ab75813 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] (Anti-NCAM1 antibody [EP2567Y] ab75813)
Predicted band size: 94 kDa
Observed band size: 180 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--immunocytochemistry-immunofluorescence-img172854.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)")
Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)
This data was developed using ab214435, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling NCAM1 with Purified ab214435 at 1:50 dilution (3.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167212.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)
This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling NCAM1 with Purified Anti-NCAM1 antibody [EP2567Y] ab75813 at 1:10000 dilution (0.0156 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-ep2567y-bsa-and-azide-free-ab214435--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167212.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (ab214435)")
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