Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)

This data was developed using ab75813, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling NCAM1 with Purified ab75813 at 1 : 10000 dilution (0.0156 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)

This data was developed using ab75813, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling NCAM1 with Purified ab75813 at 1 : 10000 dilution (0.0156 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)

This data was developed using ab214435, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling NCAM1 with Purified ab214435 at 1 : 20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)

This data was developed using ab214435, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling NCAM1 with Purified ab214435 at 1 : 50 dilution (3.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IP

Lab

Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)

This data was developed using ab75813, the same antibody clone in a different buffer formulation.

NCAM1 was immunoprecipitated from 0.35 mg SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 μg with ab75813 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab75813 IP in SH-SY5Y whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab75813 in SH-SY5Y whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] (<a href='/en-us/products/primary-antibodies/ncam1-antibody-ep2567y-ab75813'>ab75813</a>)

Predicted band size: 94 kDa

Observed band size: 180 kDa

false

• WB

Supplier Data

Western blot - Anti-NCAM1 antibody [EP2567Y] - BSA and Azide free (AB214435)

All lanes:

Western blot - Anti-NCAM1 antibody [EP2567Y] (<a href='/en-us/products/primary-antibodies/ncam1-antibody-ep2567y-ab75813'>ab75813</a>) at 1/10000 dilution

All lanes:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 94 kDa

false