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Rabbit Recombinant Monoclonal NCAM1 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (AB215981), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (AB215981), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (AB215981), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (AB215981), expandable thumbnail
  • Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (AB215981), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

15 products for Alternative Product

10 products for Alternative Version

Target data

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.(Microbial infection) Acts as a receptor for rabies virus.(Microbial infection) Acts as a receptor for Zika virus.

Alternative names

Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, NCAM1, NCAM

Recommended products

Rabbit Recombinant Monoclonal NCAM1 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP2567Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab215981 is the carrier-free version of Anti-NCAM1 antibody [EP2567Y] ab75813.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

Associated diseases and disorders

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981), expandable thumbnail

    Western blot - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

    This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NCAM1 antibody [EP2567Y] (Anti-NCAM1 antibody [EP2567Y] ab75813) at 1/10000 dilution

    All lanes: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling NCAM1 with Purified Anti-NCAM1 antibody [EP2567Y] ab75813 at 1:10000 dilution (0.0156 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.

  • Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling NCAM1 with Purified Anti-NCAM1 antibody [EP2567Y] ab75813 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

    Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling NCAM1 with Purified Anti-NCAM1 antibody [EP2567Y] ab75813 at 1:50 dilution (3.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981), expandable thumbnail

    Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

    NCAM1 was immunoprecipitated from 0.35 mg SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 μg with Anti-NCAM1 antibody [EP2567Y] ab75813 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 μg

    Lane 2: abab75813 IP in SH-SY5Y whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NCAM1 antibody [EP2567Y] ab75813 in SH-SY5Y whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.

    All lanes: Immunoprecipitation - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

    Predicted band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] - Low endotoxin, Azide free (ab215981)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling NCAM1 with Purified Anti-NCAM1 antibody [EP2567Y] ab75813 at 1:10000 dilution (0.0156 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using Anti-NCAM1 antibody [EP2567Y] ab75813, the same antibody clone in a different buffer formulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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