AB133345

Anti-NCAM1 antibody [EPR2566]

20ul selling size
RabMAb
Recombinant
What is this?

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(16 Publications)

Rabbit Recombinant Monoclonal NCAM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 16 publications.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345)

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue staining NCAM with ab133345 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human cerebellum labelling NCAM1 with ab214436 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab214436 anti NCAM1 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation (ab214436).

Unknown

Western blot - Anti-NCAM1 antibody [EPR2566] (AB133345)

All lanes:

Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

Human fetal brain cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 94 kDa

Observed band size: 140-180 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NCAM1 antibody [EPR2566]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-NCAM1 antibody [EPR2566]
Carrier free

Anti-NCAM1 antibody [EPR2566] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2566

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.

See full target information NCAM1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40868255

2025

Comparative Analysis of Davidson and Glyoxal Fixatives on Autofluorescence and Immunolabeling in Medaka () Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xiao,Eriko Sato,Ryoji Ide,Naohiro Shimamura,Chikako Saiki,Nobuhiko Miwa

Cancer medicine 12:22333-22353 PubMed38063246

2023

Hypoxia is correlated with the tumor immune microenvironment: Potential application of immunotherapy in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haotian Chen,Yao Zhang,Xingyu Chen,Runshi Xu,Yuxing Zhu,Dong He,YaXin Cheng,Zhanwang Wang,Xiang Qing,Ke Cao

Cancer cell 41:2066-2082.e9 PubMed37995683

2023

Temporal evolution reveals bifurcated lineages in aggressive neuroendocrine small cell prostate cancer trans-differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Chun Chen,Wendy Tran,Kai Song,Tyler Sugimoto,Matthew B Obusan,Liang Wang,Katherine M Sheu,Donghui Cheng,Lisa Ta,Grigor Varuzhanyan,Arthur Huang,Runzhe Xu,Yuanhong Zeng,Amirreza Borujerdpur,Nicholas A Bayley,Miyako Noguchi,Zhiyuan Mao,Colm Morrissey,Eva Corey,Peter S Nelson,Yue Zhao,Jiaoti Huang,Jung Wook Park,Owen N Witte,Thomas G Graeber

Science advances 9:eadg6693 PubMed37611092

2023

A MYCN-independent mechanism mediating secretome reprogramming and metastasis in -amplified neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Feng Zhang,Alberto Delaidelli,Sumreen Javed,Busra Turgu,Taylor Morrison,Christopher S Hughes,Xiaqiu Yang,Manideep Pachva,Michael M Lizardo,Gurdeep Singh,Jennifer Hoffmann,Yue Zhou Huang,Khushbu Patel,Rawan Shraim,Sonia H Y Kung,Gregg B Morin,Samuel Aparicio,Daniel Martinez,John M Maris,Kristopher R Bosse,Karla C Williams,Poul H Sorensen

Oncology letters 26:428 PubMed37664660

2023

Multiple synchronous primary malignant neoplasms: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Hao,Lihui Zhang,Chuanjie Xu,Min Jiang,Geyi Zhu,Jie Guo

Cell reports 42:112525 PubMed37243592

2023

Systemic alterations in neutrophils and their precursors in early-stage chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Theodore S Kapellos,Kevin Baßler,Wataru Fujii,Christina Nalkurthi,Anna C Schaar,Lorenzo Bonaguro,Tal Pecht,Izabela Galvao,Shobhit Agrawal,Adem Saglam,Erica Dudkin,Amit Frishberg,Elena de Domenico,Arik Horne,Chantal Donovan,Richard Y Kim,David Gallego-Ortega,Tessa E Gillett,Meshal Ansari,Jonas Schulte-Schrepping,Nina Offermann,Ignazio Antignano,Burcu Sivri,Wenying Lu,Mathew S Eapen,Martina van Uelft,Collins Osei-Sarpong,Maarten van den Berge,Hylke C Donker,Harry J M Groen,Sukhwinder S Sohal,Johanna Klein,Tina Schreiber,Andreas Feißt,Ali Önder Yildirim,Herbert B Schiller,Martijn C Nawijn,Matthias Becker,Kristian Händler,Marc Beyer,Melania Capasso,Thomas Ulas,Jan Hasenauer,Carmen Pizarro,Fabian J Theis,Philip M Hansbro,Dirk Skowasch,Joachim L Schultze

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:918-928 PubMed37038371

2023

A Critical Role of the Bone Marrow Envelope in Human Bone Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Levin Andersen,Pia Rosgaard Jensen,Tanja Tvistholm Sikjaer,Lars Rejnmark,Charlotte Ejersted,Jean-Marie Delaisse

Frontiers in oncology 12:960005 PubMed36439461

2022

Methylation-based reclassification and risk stratification of skull-base chordomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xulei Huo,Tengxian Guo,Ke Wang,Bohan Yao,Da Li,Huan Li,Wei Chen,Liang Wang,Zhen Wu

Scientific reports 11:4690 PubMed33633222

2021

Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro Miyazaki,Tatsuya Oda,Yuki Inagaki,Hiroko Kushige,Yutaka Saito,Nobuhito Mori,Yuzo Takayama,Yutaro Kumagai,Toutai Mitsuyama,Yasuyuki S Kida

Journal of cellular and molecular medicine 24:5146-5151 PubMed32202359

2020

Odontogenesis and neuronal differentiation characteristics of periodontal ligament stem cells from beagle dog.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Li,Dapeng Liao,Gang Sun,HanWen Chu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCAM1 antibody [EPR2566]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345)

Western blot - Anti-NCAM1 antibody [EPR2566] (AB133345)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise