Rabbit Recombinant Monoclonal NCAM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 10 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-epr2566-ab133345--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137234.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345)")
![Western blot - Anti-NCAM1 antibody [EPR2566] (AB133345), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-epr2566-ab133345--western-blot-img13381.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NCAM1 antibody [EPR2566] (AB133345)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-epr2566-ab133345--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425998.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (AB133345)")
Publications
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- Science advances 9:eadg66932023A MYCN-independent mechanism mediating secretome reprogramming and metastasis in -amplified neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Feng Zhang,Alberto Delaidelli,Sumreen Javed,Busra Turgu,Taylor Morrison,Christopher S Hughes,Xiaqiu Yang,Manideep Pachva,Michael M Lizardo,Gurdeep Singh,Jennifer Hoffmann,Yue Zhou Huang,Khushbu Patel,Rawan Shraim,Sonia H Y Kung,Gregg B Morin,Samuel Aparicio,Daniel Martinez,John M Maris,Kristopher R Bosse,Karla C Williams,Poul H SorensenPubMed 37611092
- Frontiers in oncology 12:9600052022Methylation-based reclassification and risk stratification of skull-base chordomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXulei Huo,Tengxian Guo,Ke Wang,Bohan Yao,Da Li,Huan Li,Wei Chen,Liang Wang,Zhen WuPubMed 36439461
- Scientific reports 11:46902021Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshihiro Miyazaki,Tatsuya Oda,Yuki Inagaki,Hiroko Kushige,Yutaka Saito,Nobuhito Mori,Yuzo Takayama,Yutaro Kumagai,Toutai Mitsuyama,Yasuyuki S KidaPubMed 33633222
- Journal of cellular and molecular medicine 24:5146-51512020Odontogenesis and neuronal differentiation characteristics of periodontal ligament stem cells from beagle dog.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojie Li et. al.PubMed 32202359
- The Journal of clinical endocrinology and metabolism 105:2019Scaffold-Free Endometrial Organoids Respond to Excess Androgens Associated With Polycystic Ovarian Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeerawat Wiwatpanit,Alina R Murphy,Zhenxiao Lu,Margrit Urbanek,Joanna E Burdette,Teresa K Woodruff,J Julie KimPubMed 31614364
- Annals of diagnostic pathology 38:59-612018Gene and protein expression of E-cadherin and NCAM markers in non-functioning pituitary adenomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBárbara Roberta Ongaratti,Taiana Haag,Marícia Fantinel D'Ávila,Geraldine Trott,Nelson Pires Ferreira,Carolina Garcia Soares Leães Rech,Júlia Fernanda Semmelmman Pereira-Lima,Miriam da Costa OliveiraPubMed 30419428
- Brazilian journal of medical and biological resear 51:e68082017Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesG A Mendes et. al.PubMed 29267504
- Scientific reports 7:456172017HPV positive neuroendocrine cervical cancer cells are dependent on Myc but not E6/E7 viral oncogenes.
- Oncotarget 7:34341-552016Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEloah Rabello Suarez,De Kuan Chang,Jiusong Sun,Jianhua Sui,Gordon J Freeman,Sabina Signoretti,Quan Zhu,Wayne A MarascoPubMed 27145284
- Acta orthopaedica 84:565-702013Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.
Alternative names
CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1
Recommended products
Rabbit Recombinant Monoclonal NCAM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2566
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
- Biological function summary
NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.
- Pathways
The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.
- Associated diseases and disorders
NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-epr2566-ab133345--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137234.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue staining NCAM with ab133345 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-epr2566-ab133345--western-blot-img13381.jpg "Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345)")
Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345)
All lanes: Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345) at 1/1000 dilution
Lane 1: SH-SY5Y cell lysate at 10 µg
Lane 2: Human fetal brain cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 140-180 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-epr2566-ab133345--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425998.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human cerebellum labelling NCAM1 with Anti-NCAM1 antibody [EPR2566] - BSA and Azide free ab214436 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). Anti-NCAM1 antibody [EPR2566] - BSA and Azide free ab214436 anti NCAM1 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation (Anti-NCAM1 antibody [EPR2566] - BSA and Azide free ab214436).
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