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AB6123

Anti-NCAM1 antibody [ERIC-1]

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(12 Publications)

Mouse Monoclonal NCAM1 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Tissue preparation containing NCAM1 protein.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

2 Images
Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (AB6123)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (AB6123)

Overlay histogram showing HCT116 cells stained with ab6123 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6123, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (AB6123)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (AB6123)

ab6123 staining Human normal skeletal muscle. Staining is localised the membrane.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ERIC-1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Tissue preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information.

P13591

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 99.98% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 42:133 PubMed37231509

2023

Integrated proteogenomic characterization reveals an imbalanced hepatocellular carcinoma microenvironment after incomplete radiofrequency ablation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Rong Shi,Yu-Xin Duan,Fang Cui,Zhong-Jun Wu,Mao-Ping Li,Pei-Pei Song,Qi-Ling Peng,Wen-Tao Ye,Kun-Li Yin,Mei-Qing Kang,Yan-Xi Yu,Jian Yang,Wei Tang,Rui Liao

RMD open 9: PubMed36854567

2023

Immunoproteasome subunit β5i promotes perifascicular muscle atrophy in dermatomyositis by upregulating RIG-I.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Qisheng Xia,Wenli Li,Qingyan Liu,Lining Zhang,Xiaolan Tian,Lifang Ye,Guochun Wang,Qinglin Peng

Frontiers in neurology 13:1032738 PubMed36504647

2022

The accumulation of muscle RING finger-1 in regenerating myofibers: Implications for muscle repair in immune-mediated necrotizing myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ge Yang,Qing Zhang,Hong Wang,Xue Ma,Suqiong Ji,Yue Li,Li Xu,Zhuajin Bi,Bitao Bu

Frontiers in cell and developmental biology 10:791986 PubMed35237595

2022

Endoplasmic Reticulum Stress Is Involved in Muscular Pathogenesis in Idiopathic Inflammatory Myopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Ma,Hua-Jie Gao,Qing Zhang,Meng-Ge Yang,Zhua-Jin Bi,Su-Qiong Ji,Yue Li,Li Xu,Bi-Tao Bu

Frontiers in pharmacology 12:635654 PubMed33815117

2021

The Efficacy of Tocilizumab in the Treatment of Patients with Refractory Immune-Mediated Necrotizing Myopathies: An Open-Label Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Sizhao Li,Wenli Li,Wei Jiang,Linrong He,Qinglin Peng,Guochun Wang,Xin Lu

Cell structure and function 45:143-154 PubMed32641624

2020

Prickle2 and Igsf9b Coordinately Regulate the Cytoarchitecture of the Axon Initial Segment.

Applications

Unspecified application

Species

Unspecified reactive species

Md Imrul Hasan Chowdhury,Tomoki Nishioka,Noriko Mishima,Toshihisa Ohtsuka,Kozo Kaibuchi,Daisuke Tsuboi

eLife 7: PubMed29993362

2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ren Lin,Benjamin Izar,Shu Wang,Clarence Yapp,Shaolin Mei,Parin M Shah,Sandro Santagata,Peter K Sorger

Journal of orthopaedic surgery and research 13:75 PubMed29625618

2018

Exposure to radial extracorporeal shock waves modulates viability and gene expression of human skeletal muscle cells: a controlled in vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan G Mattyasovszky,Eva K Langendorf,Ulrike Ritz,Christoph Schmitz,Irene Schmidtmann,Tobias E Nowak,Daniel Wagner,Alexander Hofmann,Pol M Rommens,Philipp Drees

Oncogene 34:3357-68 PubMed25174395

2014

A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies.

Applications

Unspecified application

Species

Unspecified reactive species

K Althoff,A Beckers,E Bell,M Nortmeyer,T Thor,A Sprüssel,S Lindner,K De Preter,A Florin,L C Heukamp,L Klein-Hitpass,K Astrahantseff,C Kumps,F Speleman,A Eggert,F Westermann,A Schramm,J H Schulte

PloS one 8:e83678 PubMed24349544

2013

Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.

Applications

IHC-Fr, ICC/IF, IHC-Fr, ICC/IF

Species

Cow, Rat, Rat, Cow

Claire Cunningham,Akshay Srivastava,Estelle Collin,Sibylle Grad,Mauro Alini,Abhay Pandit,J Gerard Wall
View all publications
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Product promise

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