Mouse Monoclonal NCAM1 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Tissue preparation containing NCAM1 protein.
Images
![Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (AB6123), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-eric-1-ab6123--flow-cytometry-img90433.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (AB6123)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (AB6123), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-eric-1-ab6123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15388.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (AB6123)")
Publications
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- RMD open 9:2023Immunoproteasome subunit β5i promotes perifascicular muscle atrophy in dermatomyositis by upregulating RIG-I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Zhang,Qisheng Xia,Wenli Li,Qingyan Liu,Lining Zhang,Xiaolan Tian,Lifang Ye,Guochun Wang,Qinglin PengPubMed 36854567
- Frontiers in neurology 13:10327382022The accumulation of muscle RING finger-1 in regenerating myofibers: Implications for muscle repair in immune-mediated necrotizing myopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng-Ge Yang,Qing Zhang,Hong Wang,Xue Ma,Suqiong Ji,Yue Li,Li Xu,Zhuajin Bi,Bitao BuPubMed 36504647
- Frontiers in cell and developmental biology 10:7919862022Endoplasmic Reticulum Stress Is Involved in Muscular Pathogenesis in Idiopathic Inflammatory Myopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Ma,Hua-Jie Gao,Qing Zhang,Meng-Ge Yang,Zhua-Jin Bi,Su-Qiong Ji,Yue Li,Li Xu,Bi-Tao BuPubMed 35237595
- Frontiers in pharmacology 12:6356542021The Efficacy of Tocilizumab in the Treatment of Patients with Refractory Immune-Mediated Necrotizing Myopathies: An Open-Label Pilot Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSizhao Li,Wenli Li,Wei Jiang,Linrong He,Qinglin Peng,Guochun Wang,Xin LuPubMed 33815117
- eLife 7:2018Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Ren Lin et. al.PubMed 29993362
- Journal of orthopaedic surgery and research 13:752018Exposure to radial extracorporeal shock waves modulates viability and gene expression of human skeletal muscle cells: a controlled in vitro study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan G Mattyasovszky et. al.PubMed 29625618
- Oncogene 34:3357-682014A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Althoff et. al.PubMed 25174395
- PloS one 8:e836782013Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.
- Journal of clinical pathology 44:580-51991Expression of neural cell adhesion molecule (NCAM) isoforms in neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE Phimister,F Kiely,J T Kemshead,K PatelPubMed 1856291
- Journal of neuro-oncology 10:111-91991A monoclonal antibody (ERIC-1), raised against retinoblastoma, that recognizes the neural cell adhesion molecule (NCAM) expressed on brain and tumours arising from the neuroectoderm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS P Bourne,K Patel,F Walsh,C J Popham,H B Coakham,J T KemsheadPubMed 1895159
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Tissue preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13591
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Reactivity data
Select an application| Flow Cyt | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
Associated Products
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Target data
Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.
Alternative names
CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1
Recommended products
Mouse Monoclonal NCAM1 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Tissue preparation containing NCAM1 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Tissue preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13591
- Clone number
- ERIC-1
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
- Biological function summary
NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.
- Pathways
The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.
- Associated diseases and disorders
NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (ab6123), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-eric-1-ab6123--flow-cytometry-img90433.jpg "Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (ab6123)")
Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (ab6123)
Overlay histogram showing HCT116 cells stained with ab6123 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6123, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (ab6123), expandable thumbnail](https://content.abcam.com/products/images/ncam1-antibody-eric-1-ab6123--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15388.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (ab6123)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (ab6123)
ab6123 staining Human normal skeletal muscle. Staining is localised the membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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