AB8233

Anti-NCAM1 antibody [MEM-188]

Name: Anti-NCAM1 antibody [MEM-188] (ab8233) | Abcam
Brand: Abcam Limited
SKU: AB8233
Rating: 5 (5 reviews)

(5 Reviews)

(2 Publications)

Mouse Monoclonal NCAM1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing NCAM1 protein.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

4 Images

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

Flow cytometry analysis (surface staining) of human peripheral blood lymphocytes with ab8233 (GAM-APC).

Flow Cyt

Unknown

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

Overlay histogram showing SH-SY5Y cells stained with ab8233 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8233, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab8233 purified antibody (concentration in sample 2 µg/ml.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab8233 purified antibody (concentration in sample 2 µg/ml).

660 APC

APC Anti-NCAM1 antibody [MEM-188]
519 FITC

FITC Anti-NCAM1 antibody [MEM-188]
778 PE/Cy7®

PE/Cy7® Anti-NCAM1 antibody [MEM-188]
675 PerCP

PerCP Anti-NCAM1 antibody [MEM-188]
Biotin

Biotin Anti-NCAM1 antibody [MEM-188]
578 PE

PE Anti-NCAM1 antibody [MEM-188]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-188

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information.

P13591

Specificity

CD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested. Positive membrane-specific staining was observed in the paraprostatic autonomic ganglion.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.

See full target information NCAM1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cutaneous pathology 43:290-2 PubMed26469404

2015

A case of acantholysis related to the internal fixation of an orthopedic steel plate acantholysis related to the bone plate.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Lu,Xue Li,Meihua Zhou,Weilin Sun,Zhonglan Su,Guoxin Song,Wenyuan Zhu

Oncotarget 6:8539-51 PubMed25890501

2015

Pharmacological inhibition of p38 MAPK reduces tumor growth in patient-derived xenografts from colon tumors.

Applications

IHC

Species

Unspecified reactive species

Jalaj Gupta,Ana Igea,Marilena Papaioannou,Pedro Pablo Lopez-Casas,Elisabet Llonch,Manuel Hidalgo,Vassilis G Gorgoulis,Angel R Nebreda

View all publications

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Assay Kits

AB315307

Human NCAM1 ELISA Kit

SimpleStep

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCAM1 antibody [MEM-188]

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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