Skip to main content

Mouse Monoclonal NCAM1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing NCAM1 protein.


Images

Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233), expandable thumbnail
  • Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233), expandable thumbnail
  • Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233), expandable thumbnail
  • Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (AB8233), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13591

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Primates
Predicted

Tested
Tested

Species
Human
Dilution info
3 µg/mL
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.

Alternative names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

Recommended products

Mouse Monoclonal NCAM1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing NCAM1 protein.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13591
Clone number
MEM-188
Purification technique
Affinity purification Protein A
Specificity

CD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested. Positive membrane-specific staining was observed in the paraprostatic autonomic ganglion.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

Associated diseases and disorders

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233), expandable thumbnail

    Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233)

    Flow cytometry analysis (surface staining) of human peripheral blood lymphocytes with ab8233 (GAM-APC).

  • Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233), expandable thumbnail

    Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233)

    Overlay histogram showing SH-SY5Y cells stained with ab8233 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8233, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233), expandable thumbnail

    Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233)

    NCAM1 Flow Cytometry staining using mouse Anti-NCAM1 antibody

    Flow cytometry surface staining pattern of human peripheral whole blood stained using ab8233 purified antibody (concentration in sample 2 µg/ml).

  • Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233), expandable thumbnail

    Flow Cytometry - Anti-NCAM1 antibody [MEM-188] (ab8233)

    NCAM1 Flow Cytometry staining using mouse Anti-NCAM1 antibody

    Flow cytometry surface staining pattern of human peripheral whole blood stained using ab8233 purified antibody (concentration in sample 2 µg/ml.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com