AB9018

Anti-NCAM1 antibody [RNL-1]

Name: Anti-NCAM1 antibody [RNL-1] (ab9018) | Abcam
Brand: Abcam Limited
SKU: AB9018
Price: 645 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(31 Publications)

Mouse Monoclonal NCAM1 antibody. Suitable for ICC, IHC-FoFr and reacts with Human, Rat samples. Cited in 31 publications. Immunogen corresponding to Cell preparation containing NCAM1 protein.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

3 Images

Supplier Data

Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (AB9018)

Immunohistochemistry of H82 cells, showing staining for NCAM1 using ab9018 (1/500).

PubMed

Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (AB9018)

Immunocytochemical analysis of Adult-Derived Human Liver Stem Cells (ADHLSC) in the left image and Hepatic Stellate Cells (HSC) in the right image. ab9018 was used to label NCAM, which is expressed in the HSC. Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. Blocking was with 3.3% hydrogen peroxide for 3 minutes. Permeabilization with D-PBS and 1% Triton X-100 for 10 minutes. Cells incubated with ab9018 at 1/100 for 1 hour. DAB staining. Counterstaining with Mayer's hematoxylin.

Image cropped from Berardis et al., Public Library of Science One, 1, e86137, Fig. 3a.; DOI: 10.1371/journal.pone.0086137 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NCAM1 antibody [RNL-1] (AB9018)

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NCAM1 antibody [RNL-1] (AB9018)

ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30μm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes : [A] 40x objective and [B] punctate cytoplasmic staining; 20x objective and [C] neuron + processes; x20 objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.

NB : We recommend indirect amplification of immunofluorescence for ab9018

Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RNL-1

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

IHC-FoFr, ICC

applications

Immunogen

Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information.

P13591

Specificity

Neural cell adhesion molecule (NCAM). ab9018 reacts with three isoforms of NCAM: NCAM-120, NCAM-140, and NCAM-180.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/200", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "Paraformaldehyde or methanol fixed cells.<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "Paraformaldehyde or methanol fixed cells.<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/100 - 1/200", "IHCFoFr-species-notes": "" } } }

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Product details

Punctuate cytoplasmic staining is observed with ab9018. Some staining of red blood cells observed.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.

See full target information NCAM1

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Archives of rheumatology 37:335-343 PubMed36589618

2022

CD14+ monocytes and soluble CD14 of synovial fluid are associated with osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ha-Reum Lee,Sunyoung Lee,In Seol Yoo,Su-Jin Yoo,Mi-Hye Kwon,Chung-Il Joung,Ji Ah Park,Seong Wook Kang,Jinhyun Kim

Neural regeneration research 17:1023-1033 PubMed34558529

2021

Nerve bundle formation during the promotion of peripheral nerve regeneration: collagen VI-neural cell adhesion molecule 1 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Hui Sun,Ming Huang,Zhou Fang,Tian-Xiao Li,Ting-Ting Wu,Yi Chen,Da-Ping Quan,Ying-Ying Xu,Yu-Ming Wang,Yi Yang,Jian-Long Zou

Nature 590:473-479 PubMed33408417

2021

Gut-licensed IFNγ NK cells drive LAMP1TRAIL anti-inflammatory astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Liliana M Sanmarco,Michael A Wheeler,Cristina Gutiérrez-Vázquez,Carolina Manganeli Polonio,Mathias Linnerbauer,Felipe A Pinho-Ribeiro,Zhaorong Li,Federico Giovannoni,Katelyn V Batterman,Giulia Scalisi,Stephanie E J Zandee,Evelyn S Heck,Moneera Alsuwailm,Douglas L Rosene,Burkhard Becher,Isaac M Chiu,Alexandre Prat,Francisco J Quintana

Annals of translational medicine 8:1513 PubMed33313258

2020

Hyperion imaging system reveals heterogeneous tumor microenvironment of oral squamous cell carcinoma patients at T1N0M0 stage.

Applications

Unspecified application

Species

Unspecified reactive species

Shang Xie,Xiao-Feng Shan,Vicky Yau,Jian-Yun Zhang,Xin-Yuan Zhang,Yong-Pan Yan,Zhi-Gang Cai

Autophagy :1-17 PubMed33030400

2020

Rapamycin prevents spontaneous abortion by triggering decidual stromal cell autophagy-mediated NK cell residence.

Applications

Unspecified application

Species

Unspecified reactive species

Han Lu,Hui-Li Yang,Wen-Jie Zhou,Zhen-Zhen Lai,Xue-Min Qiu,Qiang Fu,Jian-Yuan Zhao,Jian Wang,Da-Jin Li,Ming-Qing Li

International journal of molecular medicine 45:1909-1917 PubMed32236569

2020

Altered expression of glycoprotein non‑metastatic melanoma protein B in the distal sciatic nerve following injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yani Zheng,Chao Huang,Xiangqun Yang,Zhiying Zhang

Theranostics 10:1590-1603 PubMed32042324

2020

Aligned chitosan nanofiber hydrogel grafted with peptides mimicking bioactive brain-derived neurotrophic factor and vascular endothelial growth factor repair long-distance sciatic nerve defects in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Rao,Yanhua Wang,Dianying Zhang,Changfeng Lu,Zheng Cao,Jiajie Sui,Mengjiao Wu,Yawen Zhang,Wei Pi,Bo Wang,Yuhui Kou,Xiumei Wang,Peixun Zhang,Baoguo Jiang

Cancer science 111:219-228 PubMed31729088

2019

Low immune index correlates with favorable prognosis but with reduced benefit from chemotherapy in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wang,Xiaobo Bo,Changcheng Wang,Yanlei Xin,Lingxi Nan,Rongkui Luo,Lingli Chen,Xiao Shi,Tao Suo,Xiaoling Ni,Han Liu,Sheng Shen,Min Li,Pinxiang Lu,Yueqi Wang,Houbao Liu

Frontiers in cellular neuroscience 13:351 PubMed31456664

2019

Dl-3-n-Butylphthalide Reduces Cognitive Impairment Induced by Chronic Cerebral Hypoperfusion Through GDNF/GFRα1/Ret Signaling Preventing Hippocampal Neuron Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxian Li,Di Wei,Jiaxing Lin,Jianye Liang,Xiaomei Xie,Kangping Song,Li'an Huang

Journal of applied physiology (Bethesda, Md. : 198 126:193-201 PubMed30433863

2018

Voluntary running protects against neuromuscular dysfunction following hindlimb ischemia-reperfusion in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca J Wilson,Joshua C Drake,Di Cui,Matthew L Ritger,Yuntian Guan,Jarrod A Call,Mei Zhang,Lucia M Leitner,Axel Gödecke,Zhen Yan

View all publications

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Assay Kits

AB315307

Human NCAM1 ELISA Kit

SimpleStep

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Product promise

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