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Mouse Monoclonal NCAM1 antibody. Suitable for ICC, IHC-FoFr and reacts with Human, Rat samples. Cited in 30 publications. Immunogen corresponding to Cell preparation containing NCAM1 protein.


Images

Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (AB9018), expandable thumbnail
  • Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (AB9018), expandable thumbnail
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NCAM1 antibody [RNL-1] (AB9018), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13591

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytIHC-FoFr
Human
Tested
Not recommended
Expected
Rat
Expected
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/200

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Paraformaldehyde or methanol fixed cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

Paraformaldehyde or methanol fixed cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Rat

Dilution info

1/100 - 1/200

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.(Microbial infection) Acts as a receptor for rabies virus.(Microbial infection) Acts as a receptor for Zika virus.

Alternative names

Recommended products

Mouse Monoclonal NCAM1 antibody. Suitable for ICC, IHC-FoFr and reacts with Human, Rat samples. Cited in 30 publications. Immunogen corresponding to Cell preparation containing NCAM1 protein.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13591
Clone number

RNL-1

Purification technique

Affinity purification Protein G

Specificity

Neural cell adhesion molecule (NCAM). ab9018 reacts with three isoforms of NCAM: NCAM-120, NCAM-140, and NCAM-180.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Punctuate cytoplasmic staining is observed with ab9018. Some staining of red blood cells observed.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

Associated diseases and disorders

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

Product promise

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3 product images

  • Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018), expandable thumbnail
    Image cropped from Berardis et al., Public Library of Science One, 1, e86137, Fig. 3a.; DOI: 10.1371/journal.pone.0086137 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018)

    Immunocytochemical analysis of Adult-Derived Human Liver Stem Cells (ADHLSC) in the left image and Hepatic Stellate Cells (HSC) in the right image. ab9018 was used to label NCAM, which is expressed in the HSC. Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. Blocking was with 3.3% hydrogen peroxide for 3 minutes. Permeabilization with D-PBS and 1% Triton X-100 for 10 minutes. Cells incubated with ab9018 at 1/100 for 1 hour. DAB staining. Counterstaining with Mayer's hematoxylin.

  • Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018), expandable thumbnail

    Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018)

    Immunohistochemistry of H82 cells, showing staining for NCAM1 using ab9018 (1/500).

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NCAM1 antibody [RNL-1] (ab9018), expandable thumbnail

    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NCAM1 antibody [RNL-1] (ab9018)

    ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30μm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 40x objective and [B] punctate cytoplasmic staining; 20x objective and [C] neuron + processes; x20 objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.

    NB: We recommend indirect amplification of immunofluorescence for ab9018

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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