AB173297

Anti-NCAM2 antibody [EPR12815(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal NCAM2 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NCAM21, NCAM2, Neural cell adhesion molecule 2, N-CAM-2, NCAM-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling NCAM2 with ab173297 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized LNCaP (human prostate carcinoma epithelial cell) cells labelling NCAM2 with ab173297 at 1/200 dilution, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution.

Confocal image showing membrane staining in LNCaP cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Negative control : DU 145 (PMID : 26611261).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Magenta).

Supplier Data

Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab173297.

All lanes:

Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

Predicted band size: 93 kDa

false

Supplier Data

Western blot - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

All lanes:

Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297) at 1/1000 dilution

Lane 1:

MCF7 lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Predicted band size: 93 kDa

false

Carrier free

Anti-NCAM2 antibody [EPR12815(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12815(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM2 also called Neural Cell Adhesion Molecule 2 is a protein that facilitates cell–cell adhesion mainly in the nervous system. It belongs to the immunoglobulin superfamily and has a mass of approximately 100 kDa. This protein is present in several tissues with high expression in the brain particularly within the hippocampus and olfactory bulb. It plays an important role in the organization of synapses and neural circuit formation.

Biological function summary

NCAM2 functions as a cell adhesion molecule important for neurite outgrowth and synaptic plasticity. It does not operate alone; instead it participates in complexes with other neural cell adhesion molecules contributing to the assembly and stabilization of synaptic junctions. The protein assists neurons in maintaining their connections and supports brain development and neuroplastic changes.

Pathways

NCAM2 gets involved in both the MAPK and the PI3K-Akt signaling pathways which are essential for cell survival and growth. In these pathways NCAM2 interacts with proteins like FGF receptors and integrins facilitating the activation of downstream signaling cascades. The regulation of these pathways directly influences neuronal growth and differentiation highlighting its importance in the nervous system.

Disruptions in NCAM2 function affect conditions such as autism spectrum disorder and Alzheimer's disease. Its role in synaptic connectivity links it to cognitive and developmental disorders. In Alzheimer's disease NCAM2 connections to amyloid precursor protein processing suggest its involvement in plaque formation. Changes in NCAM2 expression and its interaction with beta-amyloid pathogenesis could contribute to synaptic dysfunction in neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.

See full target information NCAM2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC molecular and cell biology 26:6 PubMed39838328

2025

BRAF regulates circPSD3/miR-526b/RAP2A axis to hinder papillary thyroid carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Xiaojuan Zhao,Jingge Zhao,Jing Zhao,Lemei An,Gang Wu

Cell reports 40:111312 PubMed36070702

2022

Molecular convergence between Down syndrome and fragile X syndrome identified using human pluripotent stem cell models.

Applications

Unspecified application

Species

Unspecified reactive species

Sara G Susco,Sulagna Ghosh,Patrizia Mazzucato,Gabriella Angelini,Amanda Beccard,Victor Barrera,Martin H Berryer,Angelica Messana,Daisy Lam,Dane Z Hazelbaker,Lindy E Barrett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCAM2 antibody [EPR12815(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

Immunocytochemistry/ Immunofluorescence - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

Western blot - Anti-NCAM2 antibody [EPR12815(B)] (AB173297)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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