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AB137075

Anti-NCAPD2 antibody [EPR9220]

5

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal NCAPD2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CAPD2, CNAP1, KIAA0159, NCAPD2, Condensin complex subunit 1, Chromosome condensation-related SMC-associated protein 1, Chromosome-associated protein D2, Non-SMC condensin I complex subunit D2, XCAP-D2 homolog, hCAP-D2

1 Images
Western blot - Anti-NCAPD2 antibody [EPR9220] (AB137075)
  • WB

Unknown

Western blot - Anti-NCAPD2 antibody [EPR9220] (AB137075)

All lanes:

Western blot - Anti-NCAPD2 antibody [EPR9220] (ab137075) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 157 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9220

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAPD2 also known as condensation protein complex subunit 2 is a part of the condensin I complex and is important for chromosome assembly and segregation during mitosis. With an approximate molecular mass of 150 kDa NCAPD2 functions as a regulatory unit within this complex. Researchers find NCAPD2 expression highest in proliferating cells especially in tissues with active cell division such as the bone marrow and some epithelial tissues.
Biological function summary

NCAPD2 plays a significant role in maintaining chromosomal architecture during cell division. It is a part of the condensin I complex which is required for the proper condensation and stabilization of chromatids through the cell cycle. NCAPD2 interacts with other proteins within the complex such as SMC2 and SMC4 to ensure efficient DNA packaging and separation aiding in genomic stability.

Pathways

NCAPD2 is heavily involved in the cell cycle control specifically during mitosis. Its role in these pathways ensures the accurate distribution of genetic material to daughter cells. Within the cell cycle pathway NCAPD2 associates with other proteins like topoisomerase II and aurora B kinase which are important for chromosomal segregation and cytokinesis.

NCAPD2 dysregulation links to cancer and congenital malformation syndromes. Abnormal function or expression of NCAPD2 might contribute to faulty chromosome condensation leading to genomic instability a hallmark of many cancers. Additionally mutations affecting NCAPD2 and its interaction partners like SMC2 might lead to developmental disorders characterized by structural chromosome anomalies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain (PubMed : 11136719). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed : 27737959).
See full target information NCAPD2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 23:588-601 PubMed38743408

2024

NCAPD2 promotes the malignant progression of oral squamous cell carcinoma via the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Ma,Huajiao Yu,Mingda Zhu,Li Liu,Luyao Cheng,Zhengxue Han,Wulong Jin

Genetics in medicine : official journal of the American College of Medical Genetics 23:661-668 PubMed33420346

2021

Biallelic UBE4A loss-of-function variants cause intellectual disability and global developmental delay.

Applications

Unspecified application

Species

Unspecified reactive species

Uirá Souto Melo,Devon Bonner,Kevin C Kent Lloyd,Ala Moshiri,Brandon Willis,Louise Lanoue,Lynette Bower,Brian C Leonard,Davi Jardim Martins,Fernando Gomes,Felipe de Souza Leite,Danyllo Oliveira,João Paulo Kitajima,Fabiola P Monteiro,Mayana Zatz,Carlos Frederico Martins Menck,Matthew T Wheeler,Jonathan A Bernstein,Kevin Dumas,Elizabeth Spiteri,Nataliya Di Donato,Arne Jahn,Mais Hashem,Hessa S Alsaif,Aziza Chedrawi,Fowzan S Alkuraya,Fernando Kok,Heather M Byers
View all publications
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Product promise

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