Rabbit Recombinant Monoclonal NCAPD2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-NCAPD2 antibody [EPR9220] - BSA and Azide free (AB248801), expandable thumbnail](https://content.abcam.com/products/images/ncapd2-antibody-epr9220-bsa-and-azide-free-ab248801--western-blot-img57567.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NCAPD2 antibody [EPR9220] - BSA and Azide free (AB248801)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain (PubMed:11136719). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed:27737959).
Alternative names
CAPD2, CNAP1, KIAA0159, NCAPD2, Condensin complex subunit 1, Chromosome condensation-related SMC-associated protein 1, Chromosome-associated protein D2, Non-SMC condensin I complex subunit D2, XCAP-D2 homolog, hCAP-D2
Recommended products
Rabbit Recombinant Monoclonal NCAPD2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9220
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248801 is the carrier-free version of Anti-NCAPD2 antibody [EPR9220] ab137075.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCAPD2 also known as condensation protein complex subunit 2 is a part of the condensin I complex and is important for chromosome assembly and segregation during mitosis. With an approximate molecular mass of 150 kDa NCAPD2 functions as a regulatory unit within this complex. Researchers find NCAPD2 expression highest in proliferating cells especially in tissues with active cell division such as the bone marrow and some epithelial tissues.
- Biological function summary
NCAPD2 plays a significant role in maintaining chromosomal architecture during cell division. It is a part of the condensin I complex which is required for the proper condensation and stabilization of chromatids through the cell cycle. NCAPD2 interacts with other proteins within the complex such as SMC2 and SMC4 to ensure efficient DNA packaging and separation aiding in genomic stability.
- Pathways
NCAPD2 is heavily involved in the cell cycle control specifically during mitosis. Its role in these pathways ensures the accurate distribution of genetic material to daughter cells. Within the cell cycle pathway NCAPD2 associates with other proteins like topoisomerase II and aurora B kinase which are important for chromosomal segregation and cytokinesis.
- Associated diseases and disorders
NCAPD2 dysregulation links to cancer and congenital malformation syndromes. Abnormal function or expression of NCAPD2 might contribute to faulty chromosome condensation leading to genomic instability a hallmark of many cancers. Additionally mutations affecting NCAPD2 and its interaction partners like SMC2 might lead to developmental disorders characterized by structural chromosome anomalies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-NCAPD2 antibody [EPR9220] - BSA and Azide free (ab248801), expandable thumbnail](https://content.abcam.com/products/images/ncapd2-antibody-epr9220-bsa-and-azide-free-ab248801--western-blot-img57567.jpg "Western blot - Anti-NCAPD2 antibody [EPR9220] - BSA and Azide free (ab248801)")
Western blot - Anti-NCAPD2 antibody [EPR9220] - BSA and Azide free (ab248801)
This data was developed using Anti-NCAPD2 antibody [EPR9220] ab137075, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NCAPD2 antibody [EPR9220] (Anti-NCAPD2 antibody [EPR9220] ab137075) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 157 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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