AB226805

Anti-NCAPG antibody

Name: Anti-NCAPG antibody (ab226805) | Abcam
Brand: Abcam Limited
SKU: AB226805

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(7 Publications)

Rabbit Polyclonal NCAPG antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCAPG.

View Alternative Names

CAPG, NYMEL3, NCAPG, Condensin complex subunit 3, Chromosome-associated protein G, Condensin subunit CAP-G, Melanoma antigen NY-MEL-3, Non-SMC condensin I complex subunit G, XCAP-G homolog, hCAP-G

1 Images

Supplier Data

Western blot - Anti-NCAPG antibody (AB226805)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-NCAPG antibody (ab226805) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 114 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human NCAPG. The exact immunogen used to generate this antibody is proprietary information.

Q9BPX3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolar and spindle-associated protein 1 (NCAPG) is a part of the condensin complex important for chromosome condensation during mitosis and meiosis. NCAPG also known as CAP-G has a mass of approximately 110 kiloDaltons. It expresses highly in proliferating cells like those in bone marrow and the spleen. Its presence is critical during the cell cycle especially in the subphase of G2/M transition.

Biological function summary

NCAPG plays a role in maintaining chromosome architecture and stability cooperating within the condensin complex. This complex is essential for proper chromosomal segregation during cell division. It acts by introducing supercoils into the DNA promoting compaction and organizing chromatin. Incorrect functioning of NCAPG can lead to aneuploidy.

Pathways

NCAPG is a part of key regulatory circuits such as the cell cycle progression and DNA replication pathways. It interacts with other structural proteins like SMC2 and SMC4 within these pathways. These interactions are vital for replicating genetic material accurately and ensuring the proper alignment of chromosomes before cell division occurs.

NCAPG associates with tumorigenesis and cancer progression. High expression levels of NCAPG have been observed in different cancers including hepatocellular carcinoma and colorectal cancer. In these contexts the protein may interact with other significant factors such as Cyclin B1. Abnormal NCAPG activity can potentially lead to increased cell proliferation and tumor growth due to its role in chromosome stability and cell division regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

See full target information NCAPG

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 16:445-459 PubMed39744480

2025

NCAPG promotes the malignant progression of endometrioid cancer through LEF1/SEMA7A/PI3K-AKT.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Ren,Xiaohan Li,Chun Fu

Translational cancer research 13:2437-2450 PubMed38881929

2024

Up-regulation of NCAPG mediated by E2F1 facilitates the progression of osteosarcoma through the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gan Luo,Haiyang Cheng,Jigeng Fan,Tianwei Sun

Journal of translational medicine 20:325 PubMed35864529

2022

NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Rongguiyi Zhang,Jiyuan Ai,Jiakun Wang,Chi Sun,Hongcheng Lu,Aoxiao He,Min Li,Yuting Liao,Jun Lei,Fan Zhou,Linquan Wu,Wenjun Liao

Cancer cell international 22:119 PubMed35292013

2022

NCAPG facilitates colorectal cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition by activating the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlong Shi,Chang Ge,Debao Fang,Wei Wei,Li Li,Qian Wei,Hongzhu Yu

Bioengineered 13:6113-6125 PubMed35254214

2022

Increased expression of NCAPG (Non-SMC condensing I complex subunit G) is associated with progression and poor prognosis of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Wang,Xia Tian,Xufang Sui,Xiangfeng Li,Xiaoyang Zhao,Kai Han,Lingyan Sun,Yujin Dong

EBioMedicine 44:403-418 PubMed31103629

2019

HNF1A inhibition induces the resistance of pancreatic cancer cells to gemcitabine by targeting ABCB1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Lu,Dongni Xu,Jintao Peng,Zhaofan Luo,Chujie Chen,Yuqing Chen,Huimou Chen,Minghui Zheng,Peihong Yin,Zhi Wang

Cancer management and research 11:3455-3467 PubMed31114379

2019

MicroRNA‑181c suppresses growth and metastasis of hepatocellular carcinoma by modulating NCAPG.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyuan Ai,Chengwu Gong,Junjun Wu,Jun Gao,Weiwei Liu,Wenjun Liao,Linquan Wu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NCAPG antibody

Western blot - Anti-NCAPG antibody (AB226805)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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