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AB251864

Anti-NCAPG antibody

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(4 Publications)

Rabbit Polyclonal NCAPG antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCAPG aa 300-450.

View Alternative Names

CAPG, NYMEL3, NCAPG, Condensin complex subunit 3, Chromosome-associated protein G, Condensin subunit CAP-G, Melanoma antigen NY-MEL-3, Non-SMC condensin I complex subunit G, XCAP-G homolog, hCAP-G

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (AB251864)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (AB251864)

Paraffin-embedded human tonsil tissue stained for NCAPG using ab251864 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (AB251864)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (AB251864)

Paraffin-embedded human placenta tissue stained for NCAPG using ab251864 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (AB251864)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (AB251864)

Paraffin-embedded human testis tissue stained for NCAPG using ab251864 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NCAPG aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q9BPX3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolar and spindle-associated protein 1 (NCAPG) is a part of the condensin complex important for chromosome condensation during mitosis and meiosis. NCAPG also known as CAP-G has a mass of approximately 110 kiloDaltons. It expresses highly in proliferating cells like those in bone marrow and the spleen. Its presence is critical during the cell cycle especially in the subphase of G2/M transition.
Biological function summary

NCAPG plays a role in maintaining chromosome architecture and stability cooperating within the condensin complex. This complex is essential for proper chromosomal segregation during cell division. It acts by introducing supercoils into the DNA promoting compaction and organizing chromatin. Incorrect functioning of NCAPG can lead to aneuploidy.

Pathways

NCAPG is a part of key regulatory circuits such as the cell cycle progression and DNA replication pathways. It interacts with other structural proteins like SMC2 and SMC4 within these pathways. These interactions are vital for replicating genetic material accurately and ensuring the proper alignment of chromosomes before cell division occurs.

NCAPG associates with tumorigenesis and cancer progression. High expression levels of NCAPG have been observed in different cancers including hepatocellular carcinoma and colorectal cancer. In these contexts the protein may interact with other significant factors such as Cyclin B1. Abnormal NCAPG activity can potentially lead to increased cell proliferation and tumor growth due to its role in chromosome stability and cell division regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
See full target information NCAPG
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of general medicine 15:6917-6930 PubMed36061963

2022

AC099850.3/NCAPG Axis Predicts Poor Prognosis and is Associated with Resistance to EGFR Tyrosine-Kinase Inhibitors in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Bao,Yanlong Wu,Kun Zhang,Huijuan Qi

Translational cancer research 11:1630-1643 PubMed35836518

2022

Identification of novel candidate genes and small molecule drugs in ovarian cancer by bioinformatics strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wei,Xuefei Bai,Qiaomei Dong

Frontiers in oncology 12:770628 PubMed35372056

2022

NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Guangrong Zheng,Tao Han,Xiaomu Hu,Zhou Yang,Jin Wang,Zhenyi Wen,Hengyu Li,Hongjin Wang

Oncology letters 22:812 PubMed34671426

2021

MicroRNA-23b-3p targets non-SMC condensing I complex subunit G to promote proliferation and inhibit apoptosis of colorectal cancer cells via regulation of the PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peidong Li,Jing Wen,Xiru Ren,Yu Zhou,Yuan Xue,Zaihua Yan,Shengjie Li,Hongpeng Tian,Xue-Gui Tang,Guang-Jun Zhang
View all publications
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Product promise

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