Rabbit Polyclonal NCAPG antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCAPG aa 300-450.
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- Translational cancer research 11:1630-16432022Identification of novel candidate genes and small molecule drugs in ovarian cancer by bioinformatics strategy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Wei,Xuefei Bai,Qiaomei DongPubMed 35836518
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NCAPG aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BPX3
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Alternative names
CAPG, NYMEL3, NCAPG, Condensin complex subunit 3, Chromosome-associated protein G, Condensin subunit CAP-G, Melanoma antigen NY-MEL-3, Non-SMC condensin I complex subunit G, XCAP-G homolog, hCAP-G
Recommended products
Rabbit Polyclonal NCAPG antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCAPG aa 300-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NCAPG aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BPX3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nucleolar and spindle-associated protein 1 (NCAPG) is a part of the condensin complex important for chromosome condensation during mitosis and meiosis. NCAPG also known as CAP-G has a mass of approximately 110 kiloDaltons. It expresses highly in proliferating cells like those in bone marrow and the spleen. Its presence is critical during the cell cycle especially in the subphase of G2/M transition.
- Biological function summary
NCAPG plays a role in maintaining chromosome architecture and stability cooperating within the condensin complex. This complex is essential for proper chromosomal segregation during cell division. It acts by introducing supercoils into the DNA promoting compaction and organizing chromatin. Incorrect functioning of NCAPG can lead to aneuploidy.
- Pathways
NCAPG is a part of key regulatory circuits such as the cell cycle progression and DNA replication pathways. It interacts with other structural proteins like SMC2 and SMC4 within these pathways. These interactions are vital for replicating genetic material accurately and ensuring the proper alignment of chromosomes before cell division occurs.
- Associated diseases and disorders
NCAPG associates with tumorigenesis and cancer progression. High expression levels of NCAPG have been observed in different cancers including hepatocellular carcinoma and colorectal cancer. In these contexts the protein may interact with other significant factors such as Cyclin B1. Abnormal NCAPG activity can potentially lead to increased cell proliferation and tumor growth due to its role in chromosome stability and cell division regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (ab251864)
Paraffin-embedded human placenta tissue stained for NCAPG using ab251864 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (ab251864)
Paraffin-embedded human tonsil tissue stained for NCAPG using ab251864 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPG antibody (ab251864)
Paraffin-embedded human testis tissue stained for NCAPG using ab251864 at 1/50 dilution in immunohistochemical analysis.
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