Rabbit Polyclonal NCAPG2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NCAPG2.
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- Histology and histopathology 39:497-5092023miR-638 suppresses cervical cancer progression by inhibiting under the treatment of Tetrandrine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Wang,Kai Liu,Zhongming Zhou,Huizhuan GengPubMed 37702425
- Nucleic acids research 50:10680-106942022Condensin I and condensin II proteins form a LINE-1 dependent super condensin complex and cooperate to repress LINE-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacqueline R Ward,Afshin Khan,Sabrina Torres,Bert Crawford,Sarah Nock,Trenton Frisbie,John V Moran,Michelle S LongworthPubMed 36169232
- Cancers 14:2022Maintains Cancer Stemness and Promotes Erlotinib Resistance in Lung Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiyao Jiang,Jingjing Huang,Hua He,Yueying Liu,Lu Liang,Xiaoyan Sun,Yi Li,Li Cong,Bei Qing,Yiqun JiangPubMed 36139554
- Neoplasma 69:16-272021microRNA-375 inhibits the malignant behaviors of hepatic carcinoma cells by targeting NCAPG2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Tao Dai,Shu-Tong Wang,Bin Chen,Ke-Yu Tang,Nan Li,Chun-Yong Wen,Yuan Wan,Gui-Yuan Zhang,Yong-Hui Huang,Zhi-Jun GengPubMed 34818025
- Journal of cell science 132:2019Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily Deutschman et. al.PubMed 31653782
- Nature cell biology 21:1393-14022019A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristin Abramo et. al.PubMed 31685986
- Cancers 11:2019Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAllison K Baergen et. al.PubMed 31357676
- Molecular cell 40:22-332010CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase.Applications:WBReactive species:HumanRichard C Centore,Courtney G Havens,Amity L Manning,Ju-Mei Li,Rachel Litman Flynn,Alice Tse,Jianping Jin,Nicholas J Dyson,Johannes C Walter,Lee ZouPubMed 20932472
- Nature 466:508-122010PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NCAPG2. The exact immunogen used to generate this antibody is proprietary information. Database link Q86XI2
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.
Alternative names
LUZP5, NCAPG2, Condensin-2 complex subunit G2, Chromosome-associated protein G2, Leucine zipper protein 5, Non-SMC condensin II complex subunit G2, CAP-G2, hCAP-G2
Recommended products
Rabbit Polyclonal NCAPG2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NCAPG2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NCAPG2. The exact immunogen used to generate this antibody is proprietary information. Database link Q86XI2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein NCAPG2 also known as Condensin-2 complex subunit G2 is important to the mechanical processes within cells. It plays an important role in mitosis and is part of the condensin II complex responsible for chromosome condensation and stabilization during cell division. NCAPG2 is a large protein with a mass of around 120 kDa. Expression is predominantly observed in proliferating tissues such as those in the bone marrow and thymus. This wide expression reveals its direct participation in fundamental cellular functions.
- Biological function summary
The organism relies on NCAPG2 to ensure accurate chromosome segregation. As a component of the condensin II complex it collaborates with other subunits including NCAPD3 and SMC2. The protein's main task is to maintain the structural integrity of chromosomes allowing proper chromosome alignment during mitosis. This integrity is essential for genomic stability reducing the risks of aneuploidy.
- Pathways
NCAPG2 is deeply involved in the regulation of the cell cycle. It takes part in both the mitotic prophase and anaphase pathways. Its function relates closely to other proteins like Cdc2 and Cyclin B which direct the progression of cell division. By integrating with structural pathways of chromosomes NCAPG2 becomes an important element in preventing mitotic errors and preserving cellular fidelity.
- Associated diseases and disorders
NCAPG2 has been linked to carcinogenesis and some developmental disorders. Aberrant expression or dysfunction of NCAPG2 is often observed in certain cancers such as breast and prostate cancer where genomic instability is a common characteristic. In cancerous conditions its interaction with proteins like p53—known for DNA damage response—becomes significant highlighting its potential role in tumorigenesis. Furthermore studies investigate its connection with developmental anomalies probing possible implications in aberrant cellular proliferation pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunoprecipitation - Anti-NCAPG2 antibody (ab70350)
ab70350 immunoprecipitating NCAPG2 at 6 µg per reaction. Lane 1: ab70350 in HeLa whole cell extract (0.5 or 1 mg per IP reaction; 20% of IP loaded). Lane 2: IgG control. For blotting immunoprecipitated NCAPG2, ab70350 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-NCAPG2 antibody (ab70350)
Predicted band size: 131 kDa
Western blot - Anti-NCAPG2 antibody (ab70350)
All lanes: Western blot - Anti-NCAPG2 antibody (ab70350) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Predicted band size: 131 kDa
Observed band size: 131 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-NCAPG2 antibody (ab70350)
NCAPG2 western blot using anti-NCAPG2 antibody ab70350. Publication image and figure legend from Deutschman, E., Ward, J. R., et al., 2019, J Cell Sci, PubMed 31653782.
ab70350 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70350 please see the product overview.
NCAPH2 and SMC2 localize to mitochondria in human cells, while NCAPG2 does not. (A) Equal amounts of mitochondrial and cytoplasmic lysates were isolated from equal numbers of NT and NCAPH2 shRNA-expressing cells. HT-29 cells were immunoblotted with antibodies targeting NCAPH2. Immunoblot with antibodies against complex V and β-tubulin are shown to confirm the identity of mitochondrial and cytoplasmic fractions, respectively. NCAPH2 band intensities were normalized to the respective loading controls. NCAPH2 levels in isolated fractions from NCAPH2 shRNA-expressing cells were compared to levels in NT shRNA fractions, which were set to 100%. A representative of two independent experiments is shown. (B) Equal amounts of mitochondrial and cytoplasmic lysates were isolated from equal numbers of NT and NCAPG2 shRNA-expressing cells. HT-29 cells were immunoblotted with antibodies targeting NCAPG2. Immunoblot with antibodies against complex V and β-tubulin are shown to confirm the identity of mitochondrial and cytoplasmic fractions, respectively. NCAPG2 band intensities were normalized to the respective loading controls. NCAPG2 levels in isolated fractions from NCAPG2 shRNA-expressing cells were compared to levels in NT shRNA fractions, which were set to 100%. A representative of two independent experiments is shown. (C) Equal amounts of mitochondrial and cytoplasmic lysates were isolated from equal numbers of NT and SMC2 shRNA-expressing HT-29 cells immunoblotted with antibodies targeting SMC2. Immunoblot with antibodies against complex V and β-tubulin are shown to confirm the identity of mitochondrial and cytoplasmic fractions, respectively. SMC2 band intensities were normalized to the respective loading controls. SMC2 levels in isolated fractions from SMC2 shRNA-expressing cells were compared to levels in NT shRNA fractions, which were set to 100%. A representative of two independent experiments is shown.
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