JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB221722

Anti-NCAPH2 antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal NCAPH2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCAPH2 aa 350-450.

View Alternative Names

CAPH2, NCAPH2, Condensin-2 complex subunit H2, Chromosome-associated protein H2, Kleisin-beta, Non-SMC condensin II complex subunit H2, hCAP-H2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NCAPH2 antibody (AB221722)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCAPH2 antibody (AB221722)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NCAPH2 (green) using ab221722 at 4 μg/ml in ICC/IF.

Western blot - Anti-NCAPH2 antibody (AB221722)
  • WB

Supplier Data

Western blot - Anti-NCAPH2 antibody (AB221722)

All lanes:

Western blot - Anti-NCAPH2 antibody (ab221722) at 1/500 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NCAPH2 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

Q6IBW4

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAPH2 also known as non-SMC condensin II complex subunit H2 plays an important role in chromosome condensation. It is a part of the condensin II complex and contributes to the structuring and stabilization of chromatin. NCAPH2 has a mass of approximately 99 kDa and is expressed broadly in various tissues including embryonic tissues and adult organs. Its functional role is important in processes that require chromatin reorganization.
Biological function summary

NCAPH2 functions as an important component of the condensin II complex which facilitates proper chromosome segregation during cell division. The protein through its interaction with other condensin subunits C and A oversees the maintenance of chromosome architecture. It also influences mitotic progression by ensuring the accurate separation of sister chromatids. Research shows NCAPH2's involvement in maintaining genome stability highlighting its biological significance.

Pathways

NCAPH2 participates in important cellular pathways such as mitotic chromosome condensation and DNA repair mechanisms. Within these pathways NCAPH2 interacts closely with proteins like SMC2 and SMC4 which form the core of the condensin complexes. Correct functioning of these pathways ensures the integrity of genetic material during cell division preventing aneuploidy and other chromosomal abnormalities.

NCAPH2 has been linked to various conditions including cancer and developmental disorders. Its dysregulation has associations with certain cancers due to its role in maintaining genomic stability where its aberrant expression may lead to improper cell division and tumorigenesis. Additionally connections to Kleefstra syndrome where a disruption impacts chromatin organization suggest NCAPH2's interactions with other chromatin-modifying proteins including histone deacetylases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture (PubMed : 14532007). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of chromatin bridges at anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (By similarity). Seems to have lineage-specific role in T-cell development (PubMed : 14532007).
See full target information NCAPH2
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:9918 PubMed40121293

2025

Cohesin and condensin regulate chromosome topology and play an essential role in maintaining pluripotency in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eui-Hwan Choi,Keun P Kim

Cell 187:3006-3023.e26 PubMed38744280

2024

Vertebrate centromeres in mitosis are functionally bipartite structures stabilized by cohesin.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Sacristan,Kumiko Samejima,Lorena Andrade Ruiz,Moonmoon Deb,Maaike L A Lambers,Adam Buckle,Chris A Brackley,Daniel Robertson,Tetsuya Hori,Shaun Webb,Robert Kiewisz,Tristan Bepler,Eloïse van Kwawegen,Patrik Risteski,Kruno Vukušić,Iva M Tolić,Thomas Müller-Reichert,Tatsuo Fukagawa,Nick Gilbert,Davide Marenduzzo,William C Earnshaw,Geert J P L Kops

Environmental toxicology 39:2830-2841 PubMed38293837

2024

METTL3-mediated m6A modification of EPPK1 to promote the development of esophageal cancer through regulating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Jia,Longhai Yu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB200659

Anti-NCAPH2 antibody [EPR17170]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NCAPH2 antibody [EPR17170] (AB200659)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB226805

Anti-NCAPG antibody

Western blot - Anti-NCAPG antibody (AB226805)

  • 0
  • 0
Primary Antibodies

AB4448

Anti-Pericentrin antibody - Centrosome Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (AB4448)

  • 5
  • 35
Primary Antibodies

AB70350

Anti-NCAPG2 antibody

Western blot - Anti-NCAPG2 antibody (AB70350)

  • 1
  • 1
Primary Antibodies

AB154105

Anti-BRRN1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-BRRN1 antibody (AB154105)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com