AB166930

Anti-NCF1/p47-phox antibody

Name: Anti-NCF1/p47-phox antibody (ab166930) | Abcam
Brand: Abcam Limited
SKU: AB166930
Price: 535 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(13 Publications)

Goat Polyclonal NCF1/p47-phox antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human NCF1 aa 200-250.

View Alternative Names

NOXO2, SH3PXD1A, NCF1, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (AB166930)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (AB166930)

Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (AB166930)

Immunocytochemistry/ Immunofluorescence analysis of NIH3T3 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL).

Supplier Data

Western blot - Anti-NCF1/p47-phox antibody (AB166930)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-NCF1/p47-phox antibody (ab166930) at 1 µg/mL

All lanes:

Daudi cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 44 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NCF1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P14598

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab166930 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCF1 also known as p47-phox p47-phox protein or NCF-1 is a cytosolic component of the NADPH oxidase complex. It has a molecular mass of about 47 kDa. NCF1/p47-phox is expressed in neutrophils monocytes and other phagocytic cells. Functionally it plays an important role in the regulation of reactive oxygen species (ROS) production by facilitating the translocation of other subunits to the cell membrane where the NADPH oxidase complex assembles and becomes active.

Biological function summary

The NCF1/p47-phox protein plays a critical role in host defense by generating ROS which are essential for microbial killing. NCF1 is an integral part of the NADPH oxidase complex along with other subunits such as p67-phox p40-phox gp91-phox and p22-phox. When stimulated NCF1 interacts with these subunits to form an active enzyme complex that transfers electrons from NADPH to oxygen producing superoxide anion and other ROS.

Pathways

The NCF1/p47-phox component participates mainly in the NADPH oxidase activation pathway and the respiratory burst pathway. NCF1 is connected to the RAC2 GTPase which upon activation stimulates the assembly and activation of the NADPH oxidase complex at the plasma membrane. This pathway is key for the innate immune response and effective clearance of pathogens by phagocytic cells.

Mutations or dysfunction in the NCF1/p47-phox gene can lead to chronic granulomatous disease (CGD) which is characterized by a failure to produce ROS and a consequent inability to kill certain bacteria and fungi. Additionally NCF1 has links to autoimmune conditions such as rheumatoid arthritis where impaired ROS production may contribute to pathogenesis. These disease implications also involve other components of the NADPH oxidase complex such as gp91-phox whose dysfunction similarly contributes to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 2547247, PubMed : 2550933, PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed : 12732142, PubMed : 19801500).

See full target information NCF1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

mBio 16:e0327424 PubMed39679679

2024

Inhibition and evasion of neutrophil microbicidal responses by .

Applications

Unspecified application

Species

Unspecified reactive species

Hannah E Hanford,Christopher T D Price,Silvia Uriarte,Yousef Abu Kwaik

Histology and histopathology 40:757-772 PubMed39397450

2024

Ameliorative effects of HGF-overexpressed exosomes derived from ADMSCs on oxidative stress in hepatic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyu Zhou,Yanyan Wu,Junchao Xue,Liushenyan Yu

Nature communications 15:5170 PubMed38886341

2024

NCF4 attenuates colorectal cancer progression by modulating inflammasome activation and immune surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Longjun Li,Rudi Mao,Shenli Yuan,Qingqing Xie,Jinyu Meng,Yu Gu,Siyu Tan,Xiaoqing Xu,Chengjiang Gao,Hongbin Liu,Chunhong Ma,Si Ming Man,Xiangbo Meng,Tao Xu,Xiaopeng Qi

Phytotherapy research : PTR 37:3982-4001 PubMed37209001

2023

Isoamericanin A improves lipopolysaccharide-induced memory impairment in mice through suppression of the nicotinamide adenine dinucleotide phosphateoxidase-dependent nuclear factor kappa B signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqiu Yang,Ru Chen,Yue Che,Xiaohu Yao,Mingxia Fang,Yingjie Wang,Di Zhou,Ning Li,Yue Hou

American journal of physiology. Lung cellular and molecular physiology 323:L400-L409 PubMed35943724

2022

Endothelin-1 depletion of cartilage oligomeric matrix protein modulates pulmonary artery superoxide and iron metabolism-associated mitochondrial heme biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Yu,Norah Alruwaili,Melissa R Kelly,Bin Zhang,Aijing Liu,Yingqi Wang,Dong Sun,Michael S Wolin

Inflammation and regeneration 42:24 PubMed35915511

2022

Diet-induced obesity impairs spermatogenesis: the critical role of NLRP3 in Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Mu,Tai-Lang Yin,Yan Zhang,Jing Yang,Yan-Ting Wu

Science advances 7:eabl4988 PubMed34910515

2021

Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Chrysovalantou E Xirouchaki,Yaoyao Jia,Meagan J McGrath,Spencer Greatorex,Melanie Tran,Troy L Merry,Dawn Hong,Matthew J Eramo,Sophie C Broome,Jonathan S T Woodhead,Randall F D'souza,Jenny Gallagher,Ekaterina Salimova,Cheng Huang,Ralf B Schittenhelm,Junichi Sadoshima,Matthew J Watt,Christina A Mitchell,Tony Tiganis

Cell death & disease 12:1160 PubMed34907153

2021

Age-related accumulation of advanced oxidation protein products promotes osteoclastogenesis through disruption of redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingshen Zhuang,Xuebing Chen,Guixing Cai,Dizheng Wu,Chen Tu,Siyuan Zhu,Yusheng Huang,Ping Xu,Zhaoming Zhong

Aging 13:22588-22610 PubMed34584017

2021

GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Zhang,Wusan Wang,Maodi Xu,Haitang Xie,Zhichen Pu

Journal of immunology research 2020:6687143 PubMed33426093

2020

A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokang Zeng,Yan Li,Weibiao Lv,Xinhuai Dong,Chong Zeng,Liming Zeng,Zibo Wei,Xu Lin,Yanning Ma,Qiang Xiao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCF1/p47-phox antibody

Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (AB166930)

Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (AB166930)

Western blot - Anti-NCF1/p47-phox antibody (AB166930)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise