Goat Polyclonal NCF1/p47-phox antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NCF1 aa 200-250.
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- Science advances 7:eabl49882021Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChrysovalantou E Xirouchaki,Yaoyao Jia,Meagan J McGrath,Spencer Greatorex,Melanie Tran,Troy L Merry,Dawn Hong,Matthew J Eramo,Sophie C Broome,Jonathan S T Woodhead,Randall F D'souza,Jenny Gallagher,Ekaterina Salimova,Cheng Huang,Ralf B Schittenhelm,Junichi Sadoshima,Matthew J Watt,Christina A Mitchell,Tony TiganisPubMed 34910515
- Cell death & disease 12:11602021Age-related accumulation of advanced oxidation protein products promotes osteoclastogenesis through disruption of redox homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingshen Zhuang,Xuebing Chen,Guixing Cai,Dizheng Wu,Chen Tu,Siyuan Zhu,Yusheng Huang,Ping Xu,Zhaoming ZhongPubMed 34907153
- Aging 13:22588-226102021GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiwei Zhang,Wusan Wang,Maodi Xu,Haitang Xie,Zhichen PuPubMed 34584017
- Journal of immunology research 2020:66871432020A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaokang Zeng et. al.PubMed 33426093
- Frontiers in physiology 11:5752602020HIF-1α-Mediated, NADPH Oxidase-Derived ROS Contributes to Laryngeal Airway Hyperreactivity Induced by Intermittent Hypoxia in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou Shuei Lin et. al.PubMed 33117193
- Experimental and therapeutic medicine 20:1682-16922020Lipoxin A4 attenuates uric acid-activated, NADPH oxidase-dependent oxidative stress by interfering with translocation of p47phox in human umbilical vein endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou Zhou et. al.PubMed 32765680
- American journal of physiology. Cell physiology 318:C719-C7312020Carotid body type I cells engage flavoprotein and Pin1 for oxygen sensing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndré Bernardini et. al.PubMed 31967857
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NCF1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link P14598
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.03000-0.10000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Guinea pig, Cow, Pig, Chinese hamster, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Guinea pig, Cow, Pig, Chinese hamster, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Alternative names
NOXO2, SH3PXD1A, NCF1, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox
Recommended products
Goat Polyclonal NCF1/p47-phox antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NCF1 aa 200-250.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NCF1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link P14598
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab166930 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCF1 also known as p47-phox p47-phox protein or NCF-1 is a cytosolic component of the NADPH oxidase complex. It has a molecular mass of about 47 kDa. NCF1/p47-phox is expressed in neutrophils monocytes and other phagocytic cells. Functionally it plays an important role in the regulation of reactive oxygen species (ROS) production by facilitating the translocation of other subunits to the cell membrane where the NADPH oxidase complex assembles and becomes active.
- Biological function summary
The NCF1/p47-phox protein plays a critical role in host defense by generating ROS which are essential for microbial killing. NCF1 is an integral part of the NADPH oxidase complex along with other subunits such as p67-phox p40-phox gp91-phox and p22-phox. When stimulated NCF1 interacts with these subunits to form an active enzyme complex that transfers electrons from NADPH to oxygen producing superoxide anion and other ROS.
- Pathways
The NCF1/p47-phox component participates mainly in the NADPH oxidase activation pathway and the respiratory burst pathway. NCF1 is connected to the RAC2 GTPase which upon activation stimulates the assembly and activation of the NADPH oxidase complex at the plasma membrane. This pathway is key for the innate immune response and effective clearance of pathogens by phagocytic cells.
- Associated diseases and disorders
Mutations or dysfunction in the NCF1/p47-phox gene can lead to chronic granulomatous disease (CGD) which is characterized by a failure to produce ROS and a consequent inability to kill certain bacteria and fungi. Additionally NCF1 has links to autoimmune conditions such as rheumatoid arthritis where impaired ROS production may contribute to pathogenesis. These disease implications also involve other components of the NADPH oxidase complex such as gp91-phox whose dysfunction similarly contributes to these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-NCF1/p47-phox antibody (ab166930)
Detected by chemiluminescence.
All lanes: Western blot - Anti-NCF1/p47-phox antibody (ab166930) at 1 µg/mL
All lanes: Daudi cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 44 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (ab166930)
Immunocytochemistry/ Immunofluorescence analysis of NIH3T3 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL).
Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (ab166930)
Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL).
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