AB795

Anti-NCF1/p47-phox antibody

Name: Anti-NCF1/p47-phox antibody (ab795) | Abcam
Brand: Abcam Limited
SKU: AB795
Price: 565 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(23 Publications)

Goat Polyclonal NCF1/p47-phox antibody. Suitable for WB and reacts with Mouse, Pig, Human samples. Cited in 23 publications.

View Alternative Names

NOXO2, SH3PXD1A, NCF1, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

3 Images

Supplier Data

Western blot - Anti-NCF1/p47-phox antibody (AB795)

Lysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

Lane 1:

Western blot - Anti-NCF1/p47-phox antibody (ab795) at 0.2 µg/mL

Lane 2:

Western blot - Anti-NCF1/p47-phox antibody (ab795) at 0.01 µg/mL

Lanes 3 - 4:

Western blot - Anti-NCF1/p47-phox antibody (ab795) at 0.3 µg/mL

Lane 1:

U937 (human histiocytic lymphoma cell line) cell lysate at 35 µg

Lane 2:

Daudi (human Burkitt's lymphoma cell line) cell lysate at 35 µg

Lane 3:

U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate at 35 µg

Lane 4:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 35 µg

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-NCF1/p47-phox antibody (AB795)

Lysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

All lanes:

Western blot - Anti-NCF1/p47-phox antibody (ab795) at 1 µg/mL

Lane 1:

Mouse thymus lysate at 35 µg

Lane 2:

Pig spleen lysate at 35 µg

Predicted band size: 44 kDa

false

Unknown

Western blot - Anti-NCF1/p47-phox antibody (AB795)

All lanes:

Western blot - Anti-NCF1/p47-phox antibody (ab795) at 1.5 µg/mL

All lanes:

Western blot - Recombinant Human NCF1/p47-phox protein (<a href='/en-us/products/proteins-peptides/recombinant-human-ncf1-p47-phox-protein-ab98054'>ab98054</a>) at 0.1 µg

Secondary

All lanes:

Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

Predicted band size: 44 kDa

true

Exposure time: 8min

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Pig

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1.5 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1.5 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1.5 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCF1 also known as p47-phox p47-phox protein or NCF-1 is a cytosolic component of the NADPH oxidase complex. It has a molecular mass of about 47 kDa. NCF1/p47-phox is expressed in neutrophils monocytes and other phagocytic cells. Functionally it plays an important role in the regulation of reactive oxygen species (ROS) production by facilitating the translocation of other subunits to the cell membrane where the NADPH oxidase complex assembles and becomes active.

Biological function summary

The NCF1/p47-phox protein plays a critical role in host defense by generating ROS which are essential for microbial killing. NCF1 is an integral part of the NADPH oxidase complex along with other subunits such as p67-phox p40-phox gp91-phox and p22-phox. When stimulated NCF1 interacts with these subunits to form an active enzyme complex that transfers electrons from NADPH to oxygen producing superoxide anion and other ROS.

Pathways

The NCF1/p47-phox component participates mainly in the NADPH oxidase activation pathway and the respiratory burst pathway. NCF1 is connected to the RAC2 GTPase which upon activation stimulates the assembly and activation of the NADPH oxidase complex at the plasma membrane. This pathway is key for the innate immune response and effective clearance of pathogens by phagocytic cells.

Mutations or dysfunction in the NCF1/p47-phox gene can lead to chronic granulomatous disease (CGD) which is characterized by a failure to produce ROS and a consequent inability to kill certain bacteria and fungi. Additionally NCF1 has links to autoimmune conditions such as rheumatoid arthritis where impaired ROS production may contribute to pathogenesis. These disease implications also involve other components of the NADPH oxidase complex such as gp91-phox whose dysfunction similarly contributes to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

See full target information NCF1

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Phytotherapy research : PTR 38:5441-5457 PubMed39289784

2024

Geniposidic Acid Attenuates Chronic Tubulointerstitial Nephropathy Through Regulation of the NF-ƙB/Nrf2 Pathway Via Aryl Hydrocarbon Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ni Wang,Xiao-Jun Li,Wen-Feng Wang,Liang Zou,Hua Miao,Ying-Yong Zhao

Bioengineering (Basel, Switzerland) 11: PubMed38391611

2024

Intramyocardial Injection of Hypoxia-Conditioned Extracellular Vesicles Modulates Response to Oxidative Stress in the Chronically Ischemic Myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Dwight D Harris,Sharif A Sabe,Mark Broadwin,Cynthia Xu,Christopher Stone,Meghamsh Kanuparthy,Akshay Malhotra,M Ruhul Abid,Frank W Sellke

Frontiers in pharmacology 12:800522 PubMed35002735

2021

Shenkang Injection and Its Three Anthraquinones Ameliorates Renal Fibrosis by Simultaneous Targeting IƙB/NF-ƙB and Keap1/Nrf2 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Pu Luo,Ping Suo,Li-Li Ren,Hong-Jiao Liu,Yamei Zhang,Ying-Yong Zhao

Cell death & disease 12:1160 PubMed34907153

2021

Age-related accumulation of advanced oxidation protein products promotes osteoclastogenesis through disruption of redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingshen Zhuang,Xuebing Chen,Guixing Cai,Dizheng Wu,Chen Tu,Siyuan Zhu,Yusheng Huang,Ping Xu,Zhaoming Zhong

Acta physiologica (Oxford, England) 233:e13700 PubMed34089562

2021

High-intensity exercise in hypoxia improves endothelial function via increased nitric oxide bioavailability in C57BL/6 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Lavier,Manon Beaumann,Steeve Menétrey,Karima Bouzourène,Nathalie Rosenblatt-Velin,Vincent Pialoux,Lucia Mazzolai,Anne-Christine Peyter,Maxime Pellegrin,Grégoire P Millet

Frontiers in pharmacology 12:665111 PubMed34122084

2021

NaHS or Lovastatin Attenuates Cyclosporine A-Induced Hypertension in Rats by Inhibiting Epithelial Sodium Channels.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu-Shi Wang,Chen Liang,Shuai Jiang,Di Zhu,Yu Sun,Na Niu,Xu Yang,Yan-Chao Yang,Bi-Han Dong,Jie Yao,Chang-Jiang Yu,Jie Lou,Liang-Liang Tang,Ming-Ming Wu,Zhi-Ren Zhang,He-Ping Ma

Oxidative medicine and cellular longevity 2021:8899274 PubMed34007409

2021

Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yunen Liu,Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Hongxu Jin,Mingxiao Hou

Free radical biology & medicine 169:14-23 PubMed33838285

2021

Prolyl oligopeptidase inhibition reduces oxidative stress via reducing NADPH oxidase activity by activating protein phosphatase 2A.

Applications

Unspecified application

Species

Unspecified reactive species

T Eteläinen,V Kulmala,R Svarcbahs,M Jäntti,T T Myöhänen

Journal of diabetes research 2020:7574245 PubMed32851097

2020

Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Ping Chen,Jian Yang,Guo-Feng Qian,Wei-Wei Xu,Xiao-Qin Zhang

Aging 12:12684-12702 PubMed32629426

2020

Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Huyu Lin,Bin You,Xiandong Lin,Xiaohu Wang,Dongsheng Zhou,Zhiqun Chen,Yuanxiang Chen,Ren Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCF1/p47-phox antibody

Western blot - Anti-NCF1/p47-phox antibody (AB795)

Western blot - Anti-NCF1/p47-phox antibody (AB795)

Western blot - Anti-NCF1/p47-phox antibody (AB795)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

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