Rabbit Polyclonal NCF1/p47-phox phospho S304 antibody. Suitable for WB and reacts with African green monkey, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NCF1 phospho S304.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
African green monkey | Tested |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 1/500 - 1/1000 | Notes - |
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
Select an associated product type
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
NOXO2, SH3PXD1A, SH3PXD1A, NOXO2, NCF1, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox
Rabbit Polyclonal NCF1/p47-phox phospho S304 antibody. Suitable for WB and reacts with African green monkey, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NCF1 phospho S304.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Liquid
Polyclonal
Affinity purification Immunogen
Affinity purified from rabbit antiserum using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Blue Ice
+4°C
-20°C
Stable for 12 months at -20°C
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This supplementary information is collated from multiple sources and compiled automatically.
NCF1 also known as p47-phox p47-phox protein or NCF-1 is a cytosolic component of the NADPH oxidase complex. It has a molecular mass of about 47 kDa. NCF1/p47-phox is expressed in neutrophils monocytes and other phagocytic cells. Functionally it plays an important role in the regulation of reactive oxygen species (ROS) production by facilitating the translocation of other subunits to the cell membrane where the NADPH oxidase complex assembles and becomes active.
The NCF1/p47-phox protein plays a critical role in host defense by generating ROS which are essential for microbial killing. NCF1 is an integral part of the NADPH oxidase complex along with other subunits such as p67-phox p40-phox gp91-phox and p22-phox. When stimulated NCF1 interacts with these subunits to form an active enzyme complex that transfers electrons from NADPH to oxygen producing superoxide anion and other ROS.
The NCF1/p47-phox component participates mainly in the NADPH oxidase activation pathway and the respiratory burst pathway. NCF1 is connected to the RAC2 GTPase which upon activation stimulates the assembly and activation of the NADPH oxidase complex at the plasma membrane. This pathway is key for the innate immune response and effective clearance of pathogens by phagocytic cells.
Mutations or dysfunction in the NCF1/p47-phox gene can lead to chronic granulomatous disease (CGD) which is characterized by a failure to produce ROS and a consequent inability to kill certain bacteria and fungi. Additionally NCF1 has links to autoimmune conditions such as rheumatoid arthritis where impaired ROS production may contribute to pathogenesis. These disease implications also involve other components of the NADPH oxidase complex such as gp91-phox whose dysfunction similarly contributes to these disorders.
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All lanes: Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) at 1/500 dilution
Lane 1: extracts from COS7 cells treatedwith UV (15mins) at 30 µg
Lanes 2 - 3: A549 cells extracts at 30 µg
Predicted band size: 44 kDa
Observed band size: 45 kDa
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