AB63554

Anti-NCF1/p47-phox (phospho S304) antibody

Name: Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) | Abcam
Brand: Abcam Limited
SKU: AB63554
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal NCF1/p47-phox phospho S304 antibody. Suitable for WB and reacts with African green monkey, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NCF1 phospho S304.

View Alternative Names

NOXO2, SH3PXD1A, NCF1, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

2 Images

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Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (AB63554)

All lanes:

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) at 1/500 dilution

Lane 1:

extracts from COS7 cells treatedwith UV (15mins) at 30 µg

Lane 2:

A549 cells extracts at 30 µg

Lane 3:

A549 cells extracts at 30 µg with immunizing peptide

Predicted band size: 44 kDa

Observed band size: 45 kDa

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CiteAb

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (AB63554)

NCF1/p47-phox (phospho S304) western blot using anti-NCF1/p47-phox (phospho S304) antibody ab63554. Publication image and figure legend from Sowrirajan, B., Saito, Y., et al., 2017, Sci Rep, PubMed 28240310.

ab63554 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab63554 please see the product overview.

The expression of p47phox is increased in IL-27-induced macrophages (I-Mac).Monocytes were differentiated into macrophages in the absence (M-Mac) or presence of IL-27 (I-Mac). After differentiation, cells were incubated with or without IL-27. (a) M-mac, I-Mac, untreated M-Mac and IL-27-treated M-Mac were stimulated with or without 100 ng/ml PMA for 30 min at 37 °C and then ROS activity was measured by detection of secreted H2O2. Data shown represent means ± SD of triplicate samples. (b) The expression levels of p47phox were determined by western blot for M-Mac, I-Mac, untreated M-Mac and IL-27-treated M-Mac. (c) M-Mac and I-Mac were stimulated with or without PMA for 30 min, and then cell lysates were subjected to 2D gel electrophoresis. Expression of p47phox was detected by anti-p47phox and anti-phosphorylated S304 p47phox antibodies. (d) Whole cell lysates of M-Mac and I-Mac were prepared using RIPA buffer. Western blot analyses were performed using anti-SOD1, SOD2, SOD3 or anti-β-actin antibodies. The intensity of the band was analyzed by NIH Image J and normalized each SOD intensity with β-Actin.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, African green monkey

Applications

applications

Immunogen

Synthetic Peptide within Human NCF1 phospho S304. The exact immunogen used to generate this antibody is proprietary information.

P14598

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified from rabbit antiserum using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCF1 also known as p47-phox p47-phox protein or NCF-1 is a cytosolic component of the NADPH oxidase complex. It has a molecular mass of about 47 kDa. NCF1/p47-phox is expressed in neutrophils monocytes and other phagocytic cells. Functionally it plays an important role in the regulation of reactive oxygen species (ROS) production by facilitating the translocation of other subunits to the cell membrane where the NADPH oxidase complex assembles and becomes active.

Biological function summary

The NCF1/p47-phox protein plays a critical role in host defense by generating ROS which are essential for microbial killing. NCF1 is an integral part of the NADPH oxidase complex along with other subunits such as p67-phox p40-phox gp91-phox and p22-phox. When stimulated NCF1 interacts with these subunits to form an active enzyme complex that transfers electrons from NADPH to oxygen producing superoxide anion and other ROS.

Pathways

The NCF1/p47-phox component participates mainly in the NADPH oxidase activation pathway and the respiratory burst pathway. NCF1 is connected to the RAC2 GTPase which upon activation stimulates the assembly and activation of the NADPH oxidase complex at the plasma membrane. This pathway is key for the innate immune response and effective clearance of pathogens by phagocytic cells.

Mutations or dysfunction in the NCF1/p47-phox gene can lead to chronic granulomatous disease (CGD) which is characterized by a failure to produce ROS and a consequent inability to kill certain bacteria and fungi. Additionally NCF1 has links to autoimmune conditions such as rheumatoid arthritis where impaired ROS production may contribute to pathogenesis. These disease implications also involve other components of the NADPH oxidase complex such as gp91-phox whose dysfunction similarly contributes to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 2547247, PubMed : 2550933, PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed : 12732142, PubMed : 19801500).

See full target information NCF1 phospho S304

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 74:103231 PubMed38861835

2024

Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Yu Fu,Jiameng Gao,Qingqing Zhang,Nan Zhang,Zhiyuan Zhang,Xuemei Jiang,Chang Chen,Zongmei Wen

Inflammation and regeneration 42:24 PubMed35915511

2022

Diet-induced obesity impairs spermatogenesis: the critical role of NLRP3 in Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Mu,Tai-Lang Yin,Yan Zhang,Jing Yang,Yan-Ting Wu

Frontiers in pharmacology 11:556074 PubMed33658920

2021

D-4F Ameliorates Contrast Media-Induced Oxidative Injuries in Endothelial Cells via the AMPK/PKC Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yansong Guo,Wei Li,Mingming Qian,Ting Jiang,Ping Guo,Qian Du,Na Lin,Xianwei Xie,Zhiyong Wu,Donghai Lin,Donghui Liu

Free radical biology & medicine 152:186-196 PubMed32081748

2020

Geniposide protects against sepsis-induced myocardial dysfunction through AMPKα-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Song,Di-Fei Shen,Yan-Yan Meng,Chun-Yan Kong,Xin Zhang,Yu-Pei Yuan,Ling Yan,Qi-Zhu Tang,Zhen-Guo Ma

Scientific reports 7:43441 PubMed28240310

2017

Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47.

Applications

Unspecified application

Species

Unspecified reactive species

Bharatwaj Sowrirajan,Yoshiro Saito,Deepak Poudyal,Qian Chen,Hongyan Sui,Suk See DeRavin,Hiromi Imamichi,Toyotaka Sato,Douglas B Kuhns,Noriko Noguchi,Harry L Malech,H Clifford Lane,Tomozumi Imamichi

Chemico-biological interactions 189:72-81 PubMed20920494

2010

Lipopolysaccharide-induced cytokine expression in alveolar epithelial cells: role of PKCζ-mediated p47phox phosphorylation.

Applications

Species

Human

Jeremy T Leverence,Meetha Medhora,Girija G Konduri,Venkatesh Sampath

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Product promise

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Anti-NCF1/p47-phox (phospho S304) antibody

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (AB63554)

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (AB63554)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise