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AB126061

Anti-NCKAP1 antibody

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(4 Publications)

Rabbit Polyclonal NCKAP1 antibody. Suitable for WB and reacts with Rat, Mouse, Recombinant fragment, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCKAP1 aa 1-300.

View Alternative Names

HEM2, KIAA0587, NAP1, NCKAP1, Nck-associated protein 1, NAP 1, Membrane-associated protein HEM-2, p125Nap1

2 Images
Western blot - Anti-NCKAP1 antibody (AB126061)
  • WB

Supplier Data

Western blot - Anti-NCKAP1 antibody (AB126061)

Samples were separated by 5% SDS PAGE.

All lanes:

Western blot - Anti-NCKAP1 antibody (ab126061) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 129 kDa

false

Western blot - Anti-NCKAP1 antibody (AB126061)
  • WB

Supplier Data

Western blot - Anti-NCKAP1 antibody (AB126061)

Samples were separated by 5% SDS PAGE.

All lanes:

Western blot - Anti-NCKAP1 antibody (ab126061) at 1/5000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

NCKAP1 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 129 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human NCKAP1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2A7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCKAP1 also known as NAP1 serves an important role mechanically as part of the WAVE regulatory complex. It has a molecular weight of approximately 140 kDa. NCKAP1 is expressed in various tissues with higher levels noted in brain and muscle tissues. It functions in actin cytoskeleton organization by regulating the activation of the Arp2/3 complex which initiates actin polymerization.
Biological function summary

NCKAP1 contributes to cell motility and morphology by participating in lamellipodia and filopodia formation. It forms part of the WAVE complex which includes proteins such as ABI CYFIP and WASP-family proteins. This complex plays a role in rearranging the actin cytoskeleton necessary for various cell movements and shape changes important in processes like cell migration and wound healing.

Pathways

NCKAP1 interacts within the Rac1 signaling pathway. Its actions in the Rac1 pathway are important for membrane ruffling and cell spreading. NCKAP1 is also linked to the mTOR pathway through its role in regulating cytoskeletal dynamics. NCKAP1 activities are associated with other proteins like the p21-activated kinase (PAK) and Filamin A which collaborate in these signaling cascades to execute cellular responses.

NCKAP1 has been implicated in neurological disorders like autism spectrum disorder and schizophrenia. Aberrant actin dynamics regulated by NCKAP1 may contribute to the synaptic dysfunctions observed in these conditions. Furthermore its association with disease-related proteins like CYFIP1 highlights its relevance in these pathologies. Researchers continue to explore how NCKAP1's regulation of the cytoskeleton might influence disease pathways seeking potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
See full target information NCKAP1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1012564 PubMed40067817

2025

The CLIC/GEEC pathway regulates particle uptake and formation of the virus-containing compartment (VCC) in HIV-1-infected macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Kathleen Candor,Lingmei Ding,Sai Balchand,Jason E Hammonds,Paul Spearman

Journal of Cancer 14:3351-3367 PubMed37928421

2023

Pan-cancer Analysis of the Disulfidptosis-related Gene NCKAP1 and Its Prognostic Value for Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ankang Zhu,Yan Zong,Shuai Wei,Yinuo Li,Yan Fan,Shaodong Liu,Xingcai Gao

Folia histochemica et cytobiologica 59:22-29 PubMed33560516

2021

miR-140-5p inhibits the proliferation, migration and invasion of vascular smooth muscle cells by suppressing the expression of NCKAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Ma,Jiancheng Liu,Chunbo Li,Dong Wang

Cells 9: PubMed32646006

2020

The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie A Whitelaw,Karthic Swaminathan,Frieda Kage,Laura M Machesky
View all publications
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