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AB192419

Anti-NCKX2 antibody

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(2 Publications)

Rabbit Polyclonal NCKX2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLC24A2.

View Alternative Names

NCKX2, SLC24A2, Sodium/potassium/calcium exchanger 2, Na(+)/K(+)/Ca(2+)-exchange protein 2, Retinal cone Na-Ca+K exchanger, Solute carrier family 24 member 2

1 Images
Western blot - Anti-NCKX2 antibody (AB192419)
  • WB

Supplier Data

Western blot - Anti-NCKX2 antibody (AB192419)

All lanes:

Western blot - Anti-NCKX2 antibody (ab192419)

Lane 1:

MCF7 whole cell lysate

Lane 2:

Raw264.7 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 74 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SLC24A2. The exact immunogen used to generate this antibody is proprietary information.

Q9UI40

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purity is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCKX2 also known as SLC24A2 is a sodium/potassium/calcium exchanger with a molecular mass of approximately 73 kDa. This protein plays a mechanical role in transporting sodium and potassium ions in exchange for calcium ions across cellular membranes. NCKX2 is expressed in the brain especially in the neuronal tissues. It facilitates the extrusion of calcium ions which is critical in maintaining calcium homeostasis within cells.
Biological function summary

The function of NCKX2 involves regulation of intracellular calcium levels by participating in potassium and sodium exchange which is essential for proper neurological activity. NCKX2 does not operate in isolation; it often forms part of larger protein complexes that govern calcium signaling pathways. These complexes regulate synaptic transmission and plasticity influencing memory and learning.

Pathways

Functioning of NCKX2 contributes to the calcium signaling and synaptic transmission pathways. Its activity plays a pivotal role in proper neuronal signaling. NCKX2 interacts with other ion transporters and signaling molecules such as PMCA (plasma membrane calcium ATPase) in the modulation of intracellular calcium concentration. PMCA and NCKX2 together ensure efficient calcium clearance from neurons post signaling activities.

Malfunctioning of NCKX2 has associations with neurological conditions like epilepsy and bipolar disorder. Abnormal NCKX2 activity impacts calcium homeostasis contributing to the pathogenesis of these diseases. The protein is connected with other neurological proteins such as calcium/calmodulin-dependent protein kinase II (CaMKII) which also plays a role in synaptic plasticity and neuronal signaling highlighting the importance of NCKX2 in maintaining healthy neuronal functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium, potassium : sodium antiporter that transports 1 Ca(2+) and 1 K(+) in exchange for 4 Na(+) (PubMed : 10662833, PubMed : 26631410). Required for learming and memory by regulating neuronal Ca(2+), which is essential for the development of synaptic plasticity (By similarity).
See full target information SLC24A2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35806133

2022

K-Dependent Na/Ca Exchanger Isoform 2, Nckx2, Takes Part in the Neuroprotection Elicited by Ischemic Preconditioning in Brain Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Ornella Cuomo,Rossana Sirabella,Francesca Boscia,Antonella Casamassa,Jonathan Lytton,Lucio Annunziato,Giuseppe Pignataro

Molecular medicine reports 22:2115-2122 PubMed32582988

2020

A critical role for miR‑135a‑5p‑mediated regulation of SLC24A2 in neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Gang Zhou,Hui He,Pei-Ji Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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