JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327202

Anti-NCL Antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal Nucleolin antibody. Suitable for IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCL.

View Alternative Names

Nucleolin, Protein C23, NCL

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCL Antibody (AB327202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCL Antibody (AB327202)

Immunohistochemical staining of human pancreas using ab327202 at 1 : 50–1 : 200 dilution, showing strong nuclear and nucleoli positivity in exocrine glandular cells and endocrine glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCL Antibody (AB327202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCL Antibody (AB327202)

Immunohistochemical staining of human small intestine using ab327202 at 1 : 50–1 : 200 dilution, showing strong nuclear and nucleoli positivity in glandular cells and lymphoid cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCL Antibody (AB327202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCL Antibody (AB327202)

Immunohistochemical staining of human skeletal muscle using ab327202 at 1 : 50–1 : 200 dilution, showing strong nuclear and nucleoli positivity in myocytes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCL Antibody (AB327202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCL Antibody (AB327202)

Immunohistochemical staining of human lymph node using ab327202 at 1 : 50–1 : 200 dilution, showing strong nuclear and nucleoli positivity in non-germinal center cells and germinal center cells.

Immunocytochemistry/ Immunofluorescence - Anti-NCL Antibody (AB327202)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCL Antibody (AB327202)

Immunofluorescent staining of human cell line A-431 using ab327202 at 0.25–2 µg/ml, showing localization to nucleus & nucleoli.

ChIP - Anti-NCL Antibody (AB327202)
  • ChIP

Supplier Data

ChIP - Anti-NCL Antibody (AB327202)

ChIP-Exo-Seq composite graph for ab327202 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ChIP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NCL.

P19338

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
See full target information NCL
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com