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AB234709

Anti-NCM antibody

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(1 Publication)

Rabbit Polyclonal NCM antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CWC22 aa 350-650.

View Alternative Names

KIAA1604, NCM, CWC22, Pre-mRNA-splicing factor CWC22 homolog, Nucampholin homolog, fSAPb

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NCM antibody (AB234709)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCM antibody (AB234709)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NCM (green) using ab234709 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCM antibody (AB234709)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCM antibody (AB234709)

Paraffin-embedded human pancreatic cancer tissue stained for NCM using ab234709 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CWC22 aa 350-650. The exact immunogen used to generate this antibody is proprietary information.

Q9HCG8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCM also known as Neuronal Calcium Sensor-1 (NCS-1) is a protein involved in calcium-binding. It has a molecular mass of approximately 22 kDa and features three EF-hand motifs essential for its function. NCM mainly expresses in the central nervous system including areas like the cerebellum and hippocampus. It also finds expression in peripheral tissues such as the heart and adrenal gland indicating its relevance in different physiological processes.
Biological function summary

NCM modulates intracellular calcium signaling influencing processes like neurotransmitter release and synaptic plasticity. It may also participate in a complex with other proteins that affect calcium-dependent processes. NCM's role includes the regulation of ion channels and membrane trafficking events important for sustained neuronal activity. It impacts the communication between neurons and the overall nervous system function.

Pathways

NCM acts within the calcium signaling pathway and is linked to the phosphoinositide signaling pathway. NCM interacts with proteins such as the Dopamine Receptor D2 and PI4KIIIβ which are important in these pathways. This interaction allows NCM to influence various cellular responses initiated by changes in intracellular calcium levels governing diverse signaling outcomes.

NCM has associations with neurological conditions such as schizophrenia and bipolar disorder. Altered NCM function may affect calcium homeostasis in neurons contributing to these disorders. In the context of schizophrenia NCM interacts with the Dopamine Receptor D2 altering dopamine signaling implicated in the disease. Understanding NCM's relationship to these conditions can reveal potential therapeutic targets for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 12226669, PubMed : 22961380, PubMed : 28076346, PubMed : 28502770, PubMed : 29301961, PubMed : 29360106). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Promotes exon-junction complex (EJC) assembly (PubMed : 22959432, PubMed : 22961380). Hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. Through its role in EJC assembly, required for nonsense-mediated mRNA decay.
See full target information CWC22
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111086 PubMed35858576

2022

Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Gao,Jinge Gu,Hong Zhang,Kai Jiang,Linlin Tang,Ren Liu,Li Zhang,Pengfei Zhang,Cong Liu,Bin Dai,Jie Song
View all publications
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