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AB86707

Anti-NCOA4 antibody

4

(1 Review)

|

(48 Publications)

Rabbit Polyclonal NCOA4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 48 publications. Immunogen corresponding to Synthetic Peptide within Human NCOA4 aa 550 to C-terminus.

View Alternative Names

ARA70, ELE1, RFG, NCOA4, Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, Ferritin cargo receptor NCOA4, Ret-activating protein ELE1, 70 kDa AR-activator, 70 kDa androgen receptor coactivator

2 Images
Immunoprecipitation - Anti-NCOA4 antibody (AB86707)
  • IP

Unknown

Immunoprecipitation - Anti-NCOA4 antibody (AB86707)

Western Blot detection of NCOA4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86707 at 10 μg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-NCOA4 antibody (ab86707)

Predicted band size: 70 kDa

false

Western blot - Anti-NCOA4 antibody (AB86707)
  • WB

Unknown

Western blot - Anti-NCOA4 antibody (AB86707)

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Western blot - Anti-NCOA4 antibody (ab86707) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 70 kDa

Observed band size: 171 kDa,60 kDa,80 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NCOA4 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13772

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nuclear receptor coactivator 4 (NCOA4) also previously known as androgen receptor-associated protein 70 (ARA70) plays a critical role in cellular activities. It engages with nuclear receptors to enhance transcriptional activities. The molecular weight of NCOA4 is approximately 70 kDa. Scientists have observed its expression in various tissues including prostate and thyroid indicating its diverse functional capacity.
Biological function summary

NCOA4 modulates cellular iron homeostasis which is essential for cell survival and proliferation. It accomplishes this by mediating the autophagic degradation of ferritin a protein that stores iron through ferritinophagy. As part of the ferritinophagy complex NCOA4 works alongside other autophagic machinery to regulate iron bioavailability and mitigate oxidative stress maintaining cellular health and function.

Pathways

NCOA4 significantly influences the iron metabolism pathway an important component in maintaining iron balance within cells. The interaction with proteins such as ferritin highlights its role in facilitating iron release and utilization. Furthermore NCOA4 intersects with the autophagy pathway where it coordinates with autophagic proteins like LC3 to ensure proper iron recycling emphasizing its integration in cellular nutrient management.

Dysregulation of NCOA4 has links to neurodegenerative diseases like Parkinson’s disease where iron dysmetabolism plays a part in neuronal damage. Aberrant NCOA4 activity also relates to certain cancers as iron levels influence tumor growth. In these contexts NCOA4's interaction with proteins like p62 an important autophagy adaptor becomes critical highlighting its influence in disease progression and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cargo receptor for the autophagic turnover of the iron-binding ferritin complex, playing a central role in iron homeostasis (PubMed : 25327288, PubMed : 26436293). Acts as an adapter for delivery of ferritin to lysosomes and autophagic degradation of ferritin, a process named ferritinophagy (PubMed : 25327288, PubMed : 26436293). Targets the iron-binding ferritin complex to autolysosomes following starvation or iron depletion (PubMed : 25327288). Ensures efficient erythropoiesis, possibly by regulating hemin-induced erythroid differentiation (PubMed : 26436293). In some studies, has been shown to enhance the androgen receptor AR transcriptional activity as well as acting as ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma (PubMed : 10347167, PubMed : 8643607). Another study shows only weak behavior as a coactivator for the androgen receptor and no alteration of the ligand responsiveness of the AR (PubMed : 10517667). Binds to DNA replication origins, binding is not restricted to sites of active transcription and may likely be independent from the nuclear receptor transcriptional coactivator function (PubMed : 24910095). May inhibit activation of DNA replication origins, possibly by obstructing DNA unwinding via interaction with the MCM2-7 complex (PubMed : 24910095).
See full target information NCOA4
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Publications (48)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:311 PubMed40957885

2025

Targeted ferroptosis induction enhances chemotherapy efficacy in chemoresistant neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Mañas,Alexandra Seger,Aleksandra Adamska,Kyriaki Smyrilli,Joachim T Siaw,Katarzyna Radke,Erick A Muciño-Olmos,Oscar C Bedoya-Reina,Javanshir Esfandyari,Kristina Aaltonen,Daniel Bexell

Genes & genomics 47:961-972 PubMed40794264

2025

NCOA4 inhibits glioma progression by suppressing the Sonic Hedgehog pathway and its overexpression indicates a better glioma prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaining Liu,Hu Wang,Tian Qiu,Guangxiu Wang,Anling Zhang,Zhifan Jia,Xiaoguang Tong

Hereditas 162:135 PubMed40682199

2025

RBM15 promotes hypoxia/reoxygenation-induced ferroptosis in human cardiomyocytes by mediating m6A modification of ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Cheng,Jiamin Wan,Yingyue Xu,Shasha Liu,Linfeng Li,Jing Zhou,Fuyan Xie

Advanced biology 9:e70000 PubMed40464217

2025

Oncogenic miR-182-5p Targets NCOA4 to Disrupt the NCOA4-FTH1 Axis-Mediated Ferroptosis in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wulin Wen,Jia Zhao,Wuxia Zhao,Simin Zhu,Yongchun Li,Le Wang,Lina Xie,Tian Liu,Mengyu Zhang,Ruixia Ma

Cell death & disease 16:385 PubMed40374601

2025

Mechanistic insights into the role of FAT10 in modulating NCOA4-mediated ferroptosis in pancreatic acinar cells during acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingpeng Liu,Ben Che,Wenming Zhang,Dongnian Du,Dandan Zhang,Jiajuan Li,Zehao Chen,Xuzhe Yu,Miao Ye,Wei Wang,Zijing Li,Fei Xie,Qing Wang,Leifeng Chen,Jianghua Shao

Nature 642:492-500 PubMed40335696

2025

Activation of lysosomal iron triggers ferroptosis in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Cañeque,Leeroy Baron,Sebastian Müller,Alanis Carmona,Ludovic Colombeau,Antoine Versini,Stéphanie Solier,Christine Gaillet,Fabien Sindikubwabo,Julio L Sampaio,Marie Sabatier,Eikan Mishima,Armel Picard-Bernes,Laurène Syx,Nicolas Servant,Bérangère Lombard,Damarys Loew,Jiashuo Zheng,Bettina Proneth,Leishemba K Thoidingjam,Laurence Grimaud,Cameron S Fraser,Krystina J Szylo,Emma Der Kazarian,Caroline Bonnet,Emmanuelle Charafe-Jauffret,Christophe Ginestier,Patricia Santofimia-Castaño,Matias Estaras,Nelson Dusetti,Juan Lucio Iovanna,Antonio Sa Cunha,Gabriella Pittau,Pascal Hammel,Dimitri Tzanis,Sylvie Bonvalot,Sarah Watson,Vincent Gandon,Aditya Upadhyay,Derek A Pratt,Florêncio Porto Freitas,José Pedro Friedmann Angeli,Brent R Stockwell,Marcus Conrad,Jessalyn M Ubellacker,Raphaël Rodriguez

Antioxidants (Basel, Switzerland) 14: PubMed40002401

2025

Polydatin and Nicotinamide Prevent Iron Accumulation and Lipid Peroxidation in Cellular Models of Mitochondrial Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cilleros-Holgado,David Gómez-Fernández,Rocío Piñero-Pérez,José Manuel Romero-Domínguez,Diana Reche-López,Mónica Álvarez-Córdoba,Ana Romero-González,Alejandra López-Cabrera,Marta Castro De Oliveira,Andrés Rodríguez-Sacristán,Susana González-Granero,José Manuel García-Verdugo,José Antonio Sánchez-Alcázar

International journal of biological sciences 21:1275-1293 PubMed39897035

2025

Exosomal mediated lipid metabolism, ferritinophagy and CoQ-dependent pathway contributes to the ferroptosis of keratinocyte in SJS/TEN.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Pei Qiao,ChunYing Xiao,ZiPeng Cao,JiaoLing Chen,Hui Fang,JianKang Yang,ZeHua Kang,ErLe Dang,Shuai Shao,BingYu Pang,QingYang Li,ZhenLai Zhu,ShengXian Shen,Akito Hasegawa,Riichiro Abe,HongJiang Qiao,Gang Wang,Meng Fu

Frontiers in pharmacology 15:1508312 PubMed39834811

2025

Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjiao Xue,Yiwei Tian,Hua Zhang,Shijie Dai,Yangsheng Wu,Juan Jin,Jian Chen

Journal of experimental & clinical cancer research : CR 43:245 PubMed39210442

2024

CCT3/ACTN4/TFRC axis protects hepatocellular carcinoma cells from ferroptosis by inhibiting iron endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Zhu,Qiuhong Liu,Qinna Meng,Lingjian Zhang,Siwei Ju,Jiaheng Lang,Danhua Zhu,Yongxia Chen,Nadire Aishan,Xiaoxi Ouyang,Sainan Zhang,Lidan Jin,Lanlan Xiao,Linbo Wang,Lanjuan Li,Feiyang Ji
View all publications
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