Skip to main content

Rabbit Recombinant Monoclonal NCOA4 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-NCOA4 antibody [EPR28511-12] (AB314553), expandable thumbnail
  • Western blot - Anti-NCOA4 antibody [EPR28511-12] (AB314553), expandable thumbnail
  • Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (AB314553), expandable thumbnail
  • Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (AB314553), expandable thumbnail
  • Western blot - Anti-NCOA4 antibody [EPR28511-12] (AB314553), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PFlow Cyt (Intra)ICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

This product requires fresh lysates for Western blot analysis. To preserve protein degradation, harvested cells were immediately lysed, followed by rapid gel electrophoresis and membrane transfer procedures.

Species
Mouse
Dilution info
1/1000
Notes

This product requires fresh lysates for Western blot analysis. To preserve protein degradation, harvested cells were immediately lysed, followed by rapid gel electrophoresis and membrane transfer procedures.

Species
Rat
Dilution info
1/1000
Notes

This product requires fresh lysates for Western blot analysis. To preserve protein degradation, harvested cells were immediately lysed, followed by rapid gel electrophoresis and membrane transfer procedures.

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Cargo receptor for the autophagic turnover of the iron-binding ferritin complex, playing a central role in iron homeostasis (PubMed:25327288, PubMed:26436293). Acts as an adapter for delivery of ferritin to lysosomes and autophagic degradation of ferritin, a process named ferritinophagy (PubMed:25327288, PubMed:26436293). Targets the iron-binding ferritin complex to autolysosomes following starvation or iron depletion (PubMed:25327288). Ensures efficient erythropoiesis, possibly by regulating hemin-induced erythroid differentiation (PubMed:26436293). In some studies, has been shown to enhance the androgen receptor AR transcriptional activity as well as acting as ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma (PubMed:10347167, PubMed:8643607). Another study shows only weak behavior as a coactivator for the androgen receptor and no alteration of the ligand responsiveness of the AR (PubMed:10517667). Binds to DNA replication origins, binding is not restricted to sites of active transcription and may likely be independent from the nuclear receptor transcriptional coactivator function (PubMed:24910095). May inhibit activation of DNA replication origins, possibly by obstructing DNA unwinding via interaction with the MCM2-7 complex (PubMed:24910095).

Alternative names

ARA70, ELE1, RFG, NCOA4, Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, Ferritin cargo receptor NCOA4, Ret-activating protein ELE1, 70 kDa AR-activator, 70 kDa androgen receptor coactivator

Recommended products

Rabbit Recombinant Monoclonal NCOA4 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28511-12
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The nuclear receptor coactivator 4 (NCOA4) also previously known as androgen receptor-associated protein 70 (ARA70) plays a critical role in cellular activities. It engages with nuclear receptors to enhance transcriptional activities. The molecular weight of NCOA4 is approximately 70 kDa. Scientists have observed its expression in various tissues including prostate and thyroid indicating its diverse functional capacity.

Biological function summary

NCOA4 modulates cellular iron homeostasis which is essential for cell survival and proliferation. It accomplishes this by mediating the autophagic degradation of ferritin a protein that stores iron through ferritinophagy. As part of the ferritinophagy complex NCOA4 works alongside other autophagic machinery to regulate iron bioavailability and mitigate oxidative stress maintaining cellular health and function.

Pathways

NCOA4 significantly influences the iron metabolism pathway an important component in maintaining iron balance within cells. The interaction with proteins such as ferritin highlights its role in facilitating iron release and utilization. Furthermore NCOA4 intersects with the autophagy pathway where it coordinates with autophagic proteins like LC3 to ensure proper iron recycling emphasizing its integration in cellular nutrient management.

Associated diseases and disorders

Dysregulation of NCOA4 has links to neurodegenerative diseases like Parkinson’s disease where iron dysmetabolism plays a part in neuronal damage. Aberrant NCOA4 activity also relates to certain cancers as iron levels influence tumor growth. In these contexts NCOA4's interaction with proteins like p62 an important autophagy adaptor becomes critical highlighting its influence in disease progression and potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553), expandable thumbnail

    Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24695223, 31061094).
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553) at 1/1000 dilution

    Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75, 76 kDa, 76 kDa, 75 kDa

    Exposure time: 180s

  • Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553), expandable thumbnail

    Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24695223, 31061094).
    The lysates of lanes 2-3 were freshly made and used for Western blotting immediately to minimize protein degradation.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: Rat testis tissue lysate at 20 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 1 - 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 75 kDa, 76 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (ab314553), expandable thumbnail

    Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (ab314553)

    NCOA4 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab314553 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314553 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2: ab314553 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314553 in HepG2 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (ab314553) at 1/30 dilution

    All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 32s

  • Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (ab314553), expandable thumbnail

    Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (ab314553)

    NCOA4 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab314553 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314553 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2: ab314553 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314553 in NIH/3T3 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (ab314553) at 1/30 dilution

    All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 32s

  • Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553), expandable thumbnail

    Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: HT-1080.
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24695223, 31061094).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553) at 1/1000 dilution

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg

    Lane 2: A-172 (human brain glioblastoma) whole cell lysate at 20 µg

    Lane 3: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75 kDa, 76 kDa

    Exposure time: 180s

  • Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553), expandable thumbnail

    Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24695223, 31061094).
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NCOA4 antibody [EPR28511-12] (ab314553) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HepG2 transfected with siRNA specifically targeti NCOA4 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75 kDa, 76 kDa

    Exposure time: 147s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com