AB24551

Anti-NCOR2/SMRT antibody

Name: Anti-NCOR2/SMRT antibody (ab24551) | Abcam
Brand: Abcam Limited
SKU: AB24551
Price: 565 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(29 Publications)

Rabbit Polyclonal NCOR2/SMRT antibody. Suitable for WB and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCOR2 aa 1150-1400.

View Alternative Names

CTG26, NCOR2, Nuclear receptor corepressor 2, N-CoR2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, T3 receptor-associating factor, SMRT, TRAC

2 Images

Lab

Western blot - Anti-NCOR2/SMRT antibody (AB24551)

Western blot : Anti-NCOR2/SMRT antibody ab24551 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 240 kDa in Wild-type A549 cell lysates with no signal observed at this size in NCOR2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^{^®} 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-NCOR2/SMRT antibody (ab24551) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

NCOR2 knockout A549 at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

HeLa at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 274 kDa

Observed band size: 240 kDa

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Unknown

Western blot - Anti-NCOR2/SMRT antibody (AB24551)

All lanes:

Western blot - Anti-NCOR2/SMRT antibody (ab24551) at 1/1000 dilution

All lanes:

HeLa nuclear extract

Predicted band size: 275 kDa

Observed band size: 270 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human NCOR2 aa 1150-1400. The exact immunogen used to generate this antibody is proprietary information.

Q9Y618

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCOR2 also known as SMRT (Silencing Mediator for Retinoid and Thyroid receptors) is a nuclear receptor co-repressor important in gene regulation. It weighs around 270 kDa and expresses in various tissues including the brain liver and lungs. Mechanically NCOR2 interacts with nuclear receptors and other transcription factors to inhibit gene expression by facilitating the formation of a complex that deacetylates histones causing the chromatin structure to tighten and less transcription to happen.

Biological function summary

This protein functions as part of a large multi-protein complex called the SMRT/N-CoR complex. It acts by binding to un-liganded nuclear receptors such as thyroid hormone receptor and retinoic acid receptor which leads to repression of target genes essential for diverse cellular processes. This regulatory action affects cell growth differentiation and metabolism implicating NCOR2's role in maintaining normal cellular functions.

Pathways

NCOR2 participates in key pathways such as the thyroid hormone signaling pathway and the retinoic acid pathway. These pathways are critical in managing metabolism and development. NCOR2 relates to proteins like histone deacetylases (HDACs) which are part of its repression complex and key to regulating transcription. In these pathways NCOR2's interaction with nuclear receptors directs the outcome of transcriptional activity necessary for proper physiological responses.

Research links NCOR2 to various conditions including acute promyelocytic leukemia (APL) and metabolic disorders. In APL NCOR2 can form part of a fusion protein with retinoic acid receptor-alpha (RARα) disrupting normal gene regulation and promoting cancer. Furthermore its role in transcription repression connects NCOR2 to metabolic dysfunctions suggesting its potential involvement in obesity-related insulin resistance. This relationship is broadened through association with other co-repressor proteins like N-CoR emphasizing the importance of its regulatory function in health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Transcriptional corepressor that mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PubMed : 10077563, PubMed : 10097068, PubMed : 18212045, PubMed : 20812024, PubMed : 22230954, PubMed : 23911289). Acts by recruiting chromatin modifiers, such as histone deacetylases HDAC1, HDAC2 and HDAC3 (PubMed : 22230954). Required to activate the histone deacetylase activity of HDAC3 (PubMed : 22230954). Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival (PubMed : 18212045, PubMed : 23911289). Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed : 20812024).. Isoform 1. Isoform 1 and isoform 4 have different affinities for different nuclear receptors.. Isoform 4. Isoform 1 and isoform 4 have different affinities for different nuclear receptors.

See full target information NCOR2

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:6130-6142 PubMed36168631

2022

cell fate regulation by coordination of metabolic nuclear receptors during liver repair.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Qin,Shenghui Liu,Yuanyuan Lu,Yi Yan,Jing Zhang,Shiyao Cao,Mi Chen,Ning Chen,Wendong Huang,Liqiang Wang,Xiangmei Chen,Lisheng Zhang

Cell reports 37:110109 PubMed34910907

2021

Reduced NCOR2 expression accelerates androgen deprivation therapy failure in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mark D Long,Justine J Jacobi,Prashant K Singh,Gerard Llimos,Sajad A Wani,Aryn M Rowsam,Spencer R Rosario,Marlous Hoogstraat,Simon Linder,Jason Kirk,Hayley C Affronti,Andries Bergman,Wilbert Zwart,Moray J Campbell,Dominic J Smiraglia

Blood cancer journal 11:194 PubMed34864816

2021

Low NCOR2 levels in multiple myeloma patients drive multidrug resistance via MYC upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoaki Mori,Rakesh Verma,Rie Nakamoto-Matsubara,Ka Tat Siu,Cristina Panaroni,Keertik S Fulzele,Kenta Mukaihara,Chukwuamaka Onyewadume,Allison Maebius,Hiroki Kato,Lai Ping Wong,Ruslan I Sadreyev,David T Scadden,Noopur S Raje

Cancers 13: PubMed33806019

2021

Targeting the IL-6/STAT3 Signalling Cascade to Reverse Tamoxifen Resistance in Estrogen Receptor Positive Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ho Tsoi,Ellen P S Man,Ka Man Chau,Ui-Soon Khoo

Cancer cell international 20:194 PubMed32508530

2020

Ubiquitin ligase CHAF1B induces cisplatin resistance in lung adenocarcinoma by promoting NCOR2 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Lian Gong,Yi Hu,Dong He,Yuxing Zhu,Liang Xiang,Mengqing Xiao,Ying Bao,Xiaoming Liu,Qinghai Zeng,Jianye Liu,Ming Zhou,Yanhong Zhou,Yaxin Cheng,Yeyu Zhang,Liping Deng,Rongrong Zhu,Hua Lan,Ke Cao

Molecular therapy. Nucleic acids 19:1470-1481 PubMed32160715

2020

lncRNA CEBPA-AS1 Overexpression Inhibits Proliferation and Migration and Stimulates Apoptosis of OS Cells via Notch Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xia,Rui Gu,Wei Zhang,Yi-Fu Sun

Cancers 12: PubMed32110852

2020

A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2.

Applications

Unspecified application

Species

Unspecified reactive species

Man-Hong Leung,Ho Tsoi,Chun Gong,Ellen Ps Man,Stefania Zona,Shang Yao,Eric W-F Lam,Ui-Soon Khoo

Nucleic acids research 47:9573-9591 PubMed31428774

2019

PPARβ/δ recruits NCOR and regulates transcription reinitiation of ANGPTL4.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Legrand,Clemens L Bretscher,Svenja Zielke,Bernhard Wilke,Michael Daude,Barbara Fritz,Wibke E Diederich,Till Adhikary

Cancer cell 35:401-413.e6 PubMed30773341

2019

ARv7 Represses Tumor-Suppressor Genes in Castration-Resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Cato,Jonas de Tribolet-Hardy,Irene Lee,Jaice T Rottenberg,Ilsa Coleman,Diana Melchers,René Houtman,Tengfei Xiao,Wei Li,Takuma Uo,Shihua Sun,Nane C Kuznik,Bettina Göppert,Fatma Ozgun,Martin E van Royen,Adriaan B Houtsmuller,Raga Vadhi,Prakash K Rao,Lewyn Li,Steven P Balk,Robert B Den,Bruce J Trock,R Jeffrey Karnes,Robert B Jenkins,Eric A Klein,Elai Davicioni,Friederike J Gruhl,Henry W Long,X Shirley Liu,Andrew C B Cato,Nathan A Lack,Peter S Nelson,Stephen R Plymate,Anna C Groner,Myles Brown

Cell regeneration (London, England) 8:21-29 PubMed31205685

2019

Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Luo,Xiaobing Qing,Christina Benda,Zhijian Huang,Meng Zhang,Yinghua Huang,Hui Zhang,Lulu Wang,Yiwei Lai,Carl Ward,Giacomo Volpe,Xiaofen Zhong,Baoming Qin,Qiang Zhuang,Miguel A Esteban,Wenjuan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NCOR2/SMRT antibody

Western blot - Anti-NCOR2/SMRT antibody (AB24551)

Western blot - Anti-NCOR2/SMRT antibody (AB24551)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (29)

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