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AB264188

Anti-NCOR2/SMRT antibody

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Rabbit Polyclonal NCOR2/SMRT antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NCOR2 aa 1900 to C-terminus.

View Alternative Names

CTG26, NCOR2, Nuclear receptor corepressor 2, N-CoR2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, T3 receptor-associating factor, SMRT, TRAC

1 Images
Immunoprecipitation - Anti-NCOR2/SMRT antibody (AB264188)
  • IP

Supplier Data

Immunoprecipitation - Anti-NCOR2/SMRT antibody (AB264188)

NCOR2/SMRT was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264188 at 3 μg/mg lysate. An alternative NCOR2/SMRT antibody was used for western blotting at 1 μg/ml.

Lane 1 : Alternative NCOR2/SMRT antibody IP in HeLa whole cell lysate.
Lane 2 : ab264188 IP in HeLa whole cell lysate.
Lane 3 : Control IgG.

Detection : Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-NCOR2/SMRT antibody (ab264188)

Predicted band size: 275 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human NCOR2 aa 1900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y618

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCOR2 also known as SMRT (Silencing Mediator for Retinoid and Thyroid receptors) is a nuclear receptor co-repressor important in gene regulation. It weighs around 270 kDa and expresses in various tissues including the brain liver and lungs. Mechanically NCOR2 interacts with nuclear receptors and other transcription factors to inhibit gene expression by facilitating the formation of a complex that deacetylates histones causing the chromatin structure to tighten and less transcription to happen.
Biological function summary

This protein functions as part of a large multi-protein complex called the SMRT/N-CoR complex. It acts by binding to un-liganded nuclear receptors such as thyroid hormone receptor and retinoic acid receptor which leads to repression of target genes essential for diverse cellular processes. This regulatory action affects cell growth differentiation and metabolism implicating NCOR2's role in maintaining normal cellular functions.

Pathways

NCOR2 participates in key pathways such as the thyroid hormone signaling pathway and the retinoic acid pathway. These pathways are critical in managing metabolism and development. NCOR2 relates to proteins like histone deacetylases (HDACs) which are part of its repression complex and key to regulating transcription. In these pathways NCOR2's interaction with nuclear receptors directs the outcome of transcriptional activity necessary for proper physiological responses.

Research links NCOR2 to various conditions including acute promyelocytic leukemia (APL) and metabolic disorders. In APL NCOR2 can form part of a fusion protein with retinoic acid receptor-alpha (RARα) disrupting normal gene regulation and promoting cancer. Furthermore its role in transcription repression connects NCOR2 to metabolic dysfunctions suggesting its potential involvement in obesity-related insulin resistance. This relationship is broadened through association with other co-repressor proteins like N-CoR emphasizing the importance of its regulatory function in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional corepressor that mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PubMed : 10077563, PubMed : 10097068, PubMed : 18212045, PubMed : 20812024, PubMed : 22230954, PubMed : 23911289). Acts by recruiting chromatin modifiers, such as histone deacetylases HDAC1, HDAC2 and HDAC3 (PubMed : 22230954). Required to activate the histone deacetylase activity of HDAC3 (PubMed : 22230954). Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival (PubMed : 18212045, PubMed : 23911289). Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed : 20812024).. Isoform 1. Isoform 1 and isoform 4 have different affinities for different nuclear receptors.. Isoform 4. Isoform 1 and isoform 4 have different affinities for different nuclear receptors.
See full target information NCOR2
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Product promise

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